CAZyme Information: MGYG000000054_03055

Basic Information

• Species: Bacteroides acidifaciens
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides acidifaciens
• CAZyme ID: MGYG000000054_03055
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
956	MGYG000000054_44|CGC1	106838.03	4.6906

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000054	5213730	Isolate	United Kingdom	Europe

• Gene Location: Start: 10544; End: 13414 Strand: +

Full Sequence      

MVNSHIKKCLFVFFILVHWAVAFSCTSDDEALEVVELSVNKEVVDFKCEAGTQNITVSTN
APIWEATADKSWCTLSVAGKILKIFVDESEERLVREAVITITAEGLKKTVKVRQLGYEPA
ILIDTNVFEVEVIGKEISFNVTTNVEVNAGFPDWISEKVKSRAPEMVTSTHAYVVKISTL
NEKREGTIVFTEVLPEGASEESTPASASVLIVQHGLNEYNGGAGEDIEGDIKVKVVSGTD
TSHQGEDAIGKSFDGDYTTLYHSNWSNGGANYFPITLTYNFAEASDVDYLIYYPRPSGYN
GNFKEVEIQYSEDGNVFTKLLDKDFEGAATAARVTFDNTVRAKSFRFIVKSGAGDGQGFA
SCAEMEFYAKNLNAFDYSTLFEDETCSNLKVGITEADIENCKYPFFKNLAYYMLKDKYDR
NFRVADYKAFPNPDIQSETHKTNPYSLLDNPTGISVKEGETLVVMVGDTYGQNVGMKVQN
LDVPGGDGFGGVTYPLYQGINKLTMTGKGLVYVMYHTKTLVEAETARPVKIHFASGTVNG
YFDSQNVEHRGRWNELLGKATDKYFDVVGRYAHLTFETNDFRKYAATNGNELIDLYDKIA
HSEMQLLGLEKYGKMFGNRIYLNVMYHSFMYATAYHTAYNQTTMGDVCDPDVLKTTGCWG
PAHEIGHCNQTRPGIKWIGLTEVTNNIMSEYVQTTVFGQPSRIQTEDMGAVYRNRYSKAW
NGIIVPKASHANFTNIDDSNDVFCKLVPFWQLELYFGKVLGLTPLQQSDCGGFYPEVFEY
ARAKDYGGMSEGEIQLDFVYNCCVAARLNLLDFFEKWGFLTPVDKLIDDYGTKTLKITED
MAEALKKRVESLGYDKLQDTALEYISDNTWELYKTKPQVISGANATRSGNTITIKNWQNV
VVYEVRDQADNLIFISSGETTPSDTDVFTLSGNWNDGYTLYAVSAAGKRTKIPFAD

Enzyme Prediction     

No EC number prediction in MGYG000000054_03055.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CBM32	239	365	3.2e-16	0.9193548387096774

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam13402	Peptidase_M60	6.10e-58	536	818	1	268	Peptidase M60, enhancin and enhancin-like. This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonisation of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.
cd14948	BACON	1.72e-17	36	114	1	82	Bacteroidetes-Associated Carbohydrate-binding (putative) Often N-terminal (BACON) domain. The BACON domain is found in diverse domain architectures and accociated with a wide variety of domains, including carbohydrate-active enzymes and proteases. It was named for its suggested function of carbohydrate binding; the latter was inferred from domain architectures, sequence conservation, and phyletic distribution. However, recent experimental data suggest that its primary function in Bacteroides ovatus endo-xyloglucanase BoGH5A is to distance the catalytic module from the cell surface and confer additional mobility to the catalytic domain for attack of the polysaccharide. No evidence for a direct role in carbohydrate binding could be found in that case. The large majority of BACON domains are found in Bacteroidetes.
pfam00754	F5_F8_type_C	8.03e-16	241	365	6	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam18630	Peptidase_M60_C	7.18e-07	885	949	4	63	Peptidase M60 C-terminal domain. This is C-terminal domain (CTD) of M60-peptidases pfam13402. It Can also be found at the C-terminal region of gingipain B (RgpB) from P. gingivalis. It was found to possess a typical Ig-like fold encompassing seven antiparallel beta-strands organized in two beta-sheets, packed into a beta-sandwich structure that can spontaneously dimerize through C-terminal strand swapping. Translocation of gingipains from the periplasm across the OM is dependent on the conserved CTD, which appears to be important for secretion of the proteins and in particular, truncation of the last few C-terminal residues of this domain leads to accumulation of gingipains in the periplasm. Subsequently, the T9SS targeting signal was demonstrated to reside within the last 22 residues at the C-terminus of the CTD. During gingipain translocation across the OM, the CTD is cleaved off by PorU.
pfam19190	BACON_2	5.77e-06	36	117	1	91	Viral BACON domain. This family represents a distinct class of BACON domains found in crAss-like phages, the most common viral family in the human gut, in which they are found in tail fiber genes. This suggests they may play a role in phage-host interactions.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUU07877.1	0.0	1	953	1	953
QQT79916.1	0.0	7	953	2	948
QRP59789.1	0.0	7	953	2	948
QUU07870.1	0.0	24	952	19	955
QRP57133.1	0.0	24	952	19	955

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SCI_A	9.62e-76	380	856	14	477	ChainA, Peptidase M60 domain-containing protein [Akkermansia muciniphila ATCC BAA-835]
5KD2_A	5.46e-71	379	949	33	604	BT_4244metallopeptidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
5KD5_A	2.22e-69	412	949	20	556	BT_4244metallopeptidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5KD8_A BT_4244 metallopeptidase in complex with Tn antigen. [Bacteroides thetaiotaomicron VPI-5482]
7BLG_A	1.26e-67	228	370	20	162	ChainA, family 32 carbohydrate-binding module from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],7BLH_AAAA Chain AAAA, family 32 carbohydrate-binding module from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],7BLJ_A Chain A, family 32 carbohydrate-binding module from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],7BLK_A Chain A, family 32 carbohydrate-binding module from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
4A41_A	3.16e-09	233	369	30	161	CpGH89CBM32-5,from Clostridium perfringens, in complex with galactose [Clostridium perfringens],4A44_A CpGH89CBM32-5, from Clostridium perfringens, in complex with the Tn Antigen [Clostridium perfringens],4A45_A CpGH89CBM32-5, from Clostridium perfringens, in complex with GalNAc- beta-1,3-galactose [Clostridium perfringens],4AAX_A CpGH89CBM32-5, from Clostridium perfringens, in complex with N- acetylgalactosamine [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A6QLU7	5.93e-07	553	825	649	893	TRPM8 channel-associated factor 2 OS=Bos taurus OX=9913 GN=TCAF2 PE=2 SV=1
A6NFQ2	7.80e-07	545	829	653	898	TRPM8 channel-associated factor 2 OS=Homo sapiens OX=9606 GN=TCAF2 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000436	0.998749	0.000340	0.000153	0.000141	0.000144

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000054_03055.