CAZyme Information: MGYG000000054_03169

Basic Information

• Species: Bacteroides acidifaciens
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides acidifaciens
• CAZyme ID: MGYG000000054_03169
• CAZy Family: GH18
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
554	MGYG000000054_48|CGC1	60205.63	4.7641

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000054	5213730	Isolate	United Kingdom	Europe

• Gene Location: Start: 7394; End: 9058 Strand: +

Full Sequence      

MRSKFLFLCLFLFGMLTACKNTKWVDVPTGTPPEGSVTGKPGETEEPDDPDPTDKTFINC
SYIRGDFFETNRISGASMGACNDLIYLTARPYADGDLTFDLPVNDATLTGASTYAASYNG
RNGVLKLDGTGKMNAGDGLLHSPDGAFKKFTFGTYIYVSEWVDGACLFKKVDGGSVVIAF
QLGATEGNLKLTVGSATATVTNSALKSGAWHYVAVTYDGGAAKLYIDTNNTATDFTGSLP
ASVPNTRADFVMGENLKGYLDETFVNSLLMGTLGRNPISFDNWNNTKTLAYWKYDDAAKP
GKDSHTWAIRLEQIRTALNGQAGDRKIRLGIAGGEWLKMVGNATARTNFANNVKKVIEQY
NLDGADLDFEWAYSGTDLANYSKAIVQLREVLGKDVFLTVSLHPVSYKISAEAIAAVDFI
SLQCYGPSVKLFSMERFKSDGKAAVDYGIPQDKLVMGVPFYGTTGTAGEQAAYFDLVGKG
NLTNTSADTWSYEGKNYTLNSQNTIRQKTQYVCENGFGGIMSWDLATDVDVTHEMSLLKA
VKEELDYYANPTVE

Enzyme Prediction     

No EC number prediction in MGYG000000054_03169.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	282	533	4.7e-37	0.8547297297297297

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00704	Glyco_hydro_18	7.15e-28	324	528	63	307	Glycosyl hydrolases family 18.
smart00636	Glyco_18	1.03e-25	333	528	79	334	Glyco_18 domain.
cd06548	GH18_chitinase	4.08e-22	338	528	102	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
cd02872	GH18_chitolectin_chitotriosidase	6.15e-16	338	545	89	362	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.
cd00598	GH18_chitinase-like	1.21e-15	330	525	73	207	The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT71950.1	0.0	1	554	1	554
QQA10039.1	0.0	1	554	1	554
QMW88601.1	0.0	1	554	1	554
AAO76739.1	0.0	1	554	1	554
ALJ40996.1	0.0	1	554	1	554

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6XYZ_A	7.32e-16	327	544	77	304	Crystalstructure of the GH18 chitinase ChiB from the chitin utilization locus of Flavobacterium johnsoniae [Flavobacterium johnsoniae UW101]
6TSB_AAA	6.55e-12	344	528	113	337	ChainAAA, Peroxiredoxin [Clostridioides difficile 630]
6T9M_AAA	7.28e-12	344	528	132	356	ChainAAA, Peroxiredoxin [Clostridioides difficile 630]
5XWQ_A	1.62e-10	282	543	10	367	Crystalstructure of chitinase (RmChi1) from Rhizomucor miehei (sp p32 2 1, MR) [Rhizomucor miehei],5YUQ_A Chain A, Chintase [Rhizomucor miehei],5YUQ_B Chain B, Chintase [Rhizomucor miehei],7FBT_A Chain A, Chitinase [Rhizomucor miehei]
5XWF_A	2.16e-10	282	462	10	236	ChainA, Fungal chitinase from Rhizomucor miehei (SeMet-substituted proteins) [Rhizomucor miehei]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O81862	2.10e-09	326	528	91	353	Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1
Q5AM60	9.04e-09	339	545	120	372	Chitinase 4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=CHT4 PE=3 SV=1
P20533	1.15e-08	339	537	173	447	Chitinase A1 OS=Niallia circulans OX=1397 GN=chiA1 PE=1 SV=1
Q9W092	3.36e-07	316	464	109	286	Probable chitinase 2 OS=Drosophila melanogaster OX=7227 GN=Cht2 PE=1 SV=1
A6N6J0	1.63e-06	281	528	97	390	Endochitinase 46 OS=Trichoderma harzianum OX=5544 GN=chit46 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000005	0.002307	0.997723	0.000001	0.000001	0.000001

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000054_03169.