CAZyme Information: MGYG000000057_03080

Basic Information

• Species: Bacteroides sp002491635
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides sp002491635
• CAZyme ID: MGYG000000057_03080
• CAZy Family: GH9
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
836		94179.43	6.1011

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000057	4370351	Isolate	United Kingdom	Europe

• Gene Location: Start: 26807; End: 29317 Strand: -

Full Sequence      

MKSNYWILTVIFALVALPGKAGEWIRINQLGYLPQSVKVAVFMSEEGTNVGNYSLIDAFT
GKVVRTFNTAKATGKLGGMKSTYRLDFSNFTEPGTYYLKAGKTVSPRFPINAHVYNGTAD
FLLNYMRQQRCGYNPFLKDSCHIHDGYIVYHPTKTGQHIDVRGGWHDATDYLQYTTTSAN
AIYQMMFAYQENPESFGDAYDAAGLPGANGIPDIVDEIKWGLDWLNRMNPAPGELYNQIA
DDRDHAGMRLPNKDLVDYGYGPGKGRPVYFCSGEPQVRGKFRNATTGVASTAGKFASCFA
LGAKILKGYYPEFAAEIEAKADAAYQEGVKKPGACQTASVLSPYIYEEDNWVDDMELGAM
ELYKATGDNKYLAQALEYGRREPVTPWMGADSARHYQWYPFMNMGHYHLAKVDNSRISKE
FIRNMRTGIERTYEKAVENPFLHGIPYIWCSNNLTTAMLTQCRLYRETTGDDTYAEMEAS
LRDWLFGCNPWGTSMIVELPLYGDYPSQPHSSLLNAGVGNTTGGLVDGPVYRTIFENLRG
VNMTGIPGTPGQDYERFQPDLMVYHDALHDYSTNEPTMDGTACLTYYLSAMQKDGMKQAG
IPNDKNVYVDGGIIRTDPSKKQITLVFTAADKADGADAIISTLKKHGIKGGFFFTGEFYE
LYPDVVKRLLDEGHFVGSHSYGHLLYMPWEDRDSLLVTREEFENDIMKSYETLRKAGIEY
KDAPVYIPPYEYYNKEISAWAKNMGIQVINYTPGTMSNADYTTPDMGQKYRSSKFIYDKI
MEVEKKEGLNGHLMLIHFGTDDRRTDKFYNGYLDKMIKTLKQKGYTFVPVCEAVGI

Enzyme Prediction     

No EC number prediction in MGYG000000057_03080.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH9	115	588	8.8e-77	0.992822966507177
CE4	617	749	4.5e-18	0.9230769230769231

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam00759	Glyco_hydro_9	8.40e-64	123	588	7	374	Glycosyl hydrolase family 9.
pfam02927	CelD_N	5.92e-30	24	105	2	83	Cellulase N-terminal ig-like domain.
cd02850	E_set_Cellulase_N	4.48e-28	24	110	1	86	N-terminal Early set domain associated with the catalytic domain of cellulase. E or "early" set domains are associated with the catalytic domain of cellulases at the N-terminal end. Cellulases are O-glycosyl hydrolases (GHs) that hydrolyze beta 1-4 glucosidic bonds in cellulose. They are usually categorized into either exoglucanases, which sequentially release terminal sugar units from the cellulose chain, or endoglucanases, which also attack the chain internally. The N-terminal domain of cellulase may be related to the immunoglobulin and/or fibronectin type III superfamilies. These domains are associated with different types of catalytic domains at either the N-terminal or C-terminal end and may be involved in homodimeric/tetrameric/dodecameric interactions. Members of this family include members of the alpha amylase family, sialidase, galactose oxidase, cellulase, cellulose, hyaluronate lyase, chitobiase, and chitinase, among others.
cd10917	CE4_NodB_like_6s_7s	1.17e-26	639	821	19	171	Catalytic NodB homology domain of rhizobial NodB-like proteins. This family belongs to the large and functionally diverse carbohydrate esterase 4 (CE4) superfamily, whose members show strong sequence similarity with some variability due to their distinct carbohydrate substrates. It includes many rhizobial NodB chitooligosaccharide N-deacetylase (EC 3.5.1.-)-like proteins, mainly from bacteria and eukaryotes, such as chitin deacetylases (EC 3.5.1.41), bacterial peptidoglycan N-acetylglucosamine deacetylases (EC 3.5.1.-), and acetylxylan esterases (EC 3.1.1.72), which catalyze the N- or O-deacetylation of substrates such as acetylated chitin, peptidoglycan, and acetylated xylan. All members of this family contain a catalytic NodB homology domain with the same overall topology and a deformed (beta/alpha)8 barrel fold with 6- or 7 strands. Their catalytic activity is dependent on the presence of a divalent cation, preferably cobalt or zinc, and they employ a conserved His-His-Asp zinc-binding triad closely associated with the conserved catalytic base (aspartic acid) and acid (histidine) to carry out acid/base catalysis. Several family members show diversity both in metal ion specificities and in the residues that coordinate the metal.
cd10950	CE4_BsYlxY_like	5.57e-23	616	832	1	186	Putative catalytic NodB homology domain of uncharacterized protein YlxY from Bacillus subtilis and its bacterial homologs. The Bacillus subtilis genome contains six polysaccharide deacetylase gene homologs: pdaA, pdaB (previously known as ybaN), yheN, yjeA, yxkH and ylxY. This family is represented by Bacillus subtilis putative polysaccharide deacetylase BsYlxY, encoded by the ylxY gene, which is a member of the carbohydrate esterase 4 (CE4) superfamily. Although its biological function still remains unknown, BsYlxY shows high sequence homology to the catalytic domain of Bacillus subtilis pdaB gene encoding a putative polysaccharide deacetylase (BsPdaB), which is essential for the maintenance of spores after the late stage of sporulation and is highly conserved in spore-forming bacteria. However, disruption of the ylxY gene in B. subtilis did not cause any sporulation defect. Moreover, the Asp residue in the classical His-His-Asp zinc-binding motif of CE4 esterases is mutated to a Val residue in this family. Other catalytically relevant residues of CE4 esterases are also not conserved, which suggest that members of this family may be inactive.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBJ19309.1	0.0	1	836	1	836
QMI80662.1	0.0	1	836	1	836
QUT35459.1	0.0	1	836	1	836
QUT98069.1	0.0	1	836	1	836
QPH59586.1	0.0	1	836	1	836

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3X17_A	1.48e-35	22	586	15	551	Crystalstructure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium],3X17_B Crystal structure of metagenome-derived glycoside hydrolase family 9 endoglucanase [uncultured bacterium]
5U2O_A	1.49e-28	33	594	2	542	Crystalstructure of Zn-binding triple mutant of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
5U0H_A	3.38e-25	33	594	2	542	Crystalstructure of GH family 9 endoglucanase J30 [Thermobacillus composti KWC4]
4CJ0_A	2.78e-22	25	610	30	561	ChainA, ENDOGLUCANASE D [Acetivibrio thermocellus],4CJ1_A Chain A, ENDOGLUCANASE D [Acetivibrio thermocellus]
1CLC_A	2.92e-22	25	610	44	575	ChainA, ENDOGLUCANASE CELD; EC: 3.2.1.4 [Acetivibrio thermocellus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0C2S4	1.52e-21	25	610	30	561	Endoglucanase D (Fragment) OS=Acetivibrio thermocellus OX=1515 GN=celD PE=1 SV=1
A3DDN1	2.18e-21	25	589	54	569	Endoglucanase D OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celD PE=1 SV=1
A3DCH1	1.76e-20	25	510	214	737	Cellulose 1,4-beta-cellobiosidase OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celK PE=3 SV=1
P23658	1.83e-20	25	591	4	545	Cellodextrinase OS=Butyrivibrio fibrisolvens OX=831 GN=ced1 PE=1 SV=1
P0C2S1	5.30e-20	25	510	214	737	Cellulose 1,4-beta-cellobiosidase OS=Acetivibrio thermocellus OX=1515 GN=celK PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000308	0.999023	0.000195	0.000142	0.000149	0.000143

TMHMM  Annotations     

start	end
5	27