CAZyme Information: MGYG000000059_00299

Basic Information

• Species: Lawsonibacter sp900066645
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; Lawsonibacter; Lawsonibacter sp900066645
• CAZyme ID: MGYG000000059_00299
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
886		97807.12	4.4957

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000059	2886884	Isolate	United Kingdom	Europe

• Gene Location: Start: 318830; End: 321490 Strand: -

Full Sequence      

MSDQNNNNSLYENSEYPSRQRRTADQHPRRRPRKKRGSTGNTILKVLGTLFLVGLCTGAV
LCCFGAYYVQTVILPEVEVSMDDFQLGENSVMYYQDKETGRYVEMTTLLSSTSSVWVDLE
EMPKYLPQAAVAIEDQRFYTHPGVDWKRTAKAVLDMFLGNDISGGSTITQQLIKNMTDYN
ETTVKRKVTEIFRAIRFTQNNSKDDTLEMYLNIIPLGAGCEGVGSASLKYFGKPVSELTL
AECASLVGITNNPSKYGPYSFAKSKTSTGEVWDARQWNKYRQEVILGEMLKQGMISQEEH
DEAVAQELVFVRAEGEEAETEIYNWYEETVISDVRADLKEKYGFSDSRLDQIMARGGLRI
YTCMDPEIQAIAEAVYEDRANLNYTSKSGQQMQSSITIIDNETGDVAAIVGRFGEKTENR
GMNYANAAKRQPGSSIKPLTVYAPALEKGLLSPISVLDDYPYDTRTGSAWPLNSGGNRYR
GLTTLKDGLMRSANTIAVRVVADYVTPQESFSFGKDKFKLDLVDAVQSGDQIMSDIDISP
MAMGGLTAGVSSRTMAQAYATFPSAGVYTYSRTYTRVTQLVDGEEVVLLENEPEKEQVLK
ESTAYYMNTMLQRVISGSGVSGDGHTGYEAALSGMRAAGKTGTTSDNFDRWFVGYTPYYT
AAVWTGYEQNEKMKTGSANPAAVLWKKVMSQVHEGLENKKFPEVSGLKQVTYCLDSGLLA
GEYCALDPRGSRVATDYIFPEDYPDGRTCTVHTAESVVKVCVDSPILNEDGTETGLYHIA
GPYCPEESLREVCYPNYEREKVGSAVAQDEMYRFDVVEAQGTCTVHTEAPVVQPDPSDPN
FPWDPSQPVDPNDPGSGSQTDPSGSGSGSGSDSPPPSQDIPQDQWP

Enzyme Prediction     

No EC number prediction in MGYG000000059_00299.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	110	289	8.8e-56	0.943502824858757

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	1.16e-160	43	703	14	607	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	9.30e-160	115	695	3	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	1.49e-112	43	722	8	748	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	5.32e-67	109	290	8	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
TIGR02073	PBP_1c	7.32e-67	116	730	35	578	penicillin-binding protein 1C. This subfamily of the penicillin binding proteins includes the member from E. coli designated penicillin-binding protein 1C. Members have both transglycosylase and transpeptidase domains and are involved in forming cross-links in the late stages of peptidoglycan biosynthesis. All members of this subfamily are presumed to have the same basic function. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIA31344.1	4.99e-253	27	840	10	821
ANU41033.1	2.92e-251	42	840	5	797
ALP94484.1	2.51e-239	28	839	16	826
QBB65500.1	5.03e-239	45	839	3	794
QUO37805.1	4.17e-203	28	837	14	822

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7U4H_A	2.73e-68	117	722	43	791	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3ZG8_B	1.47e-56	202	705	8	470	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3ZG7_B	2.72e-49	202	672	8	437	CrystalStructure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the apo form [Listeria monocytogenes]
3DWK_A	2.59e-42	117	706	30	604	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
3UDF_A	9.69e-42	114	695	39	718	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0SRL7	1.12e-96	30	757	11	729	Penicillin-binding protein 1A OS=Clostridium perfringens (strain SM101 / Type A) OX=289380 GN=pbpA PE=3 SV=1
Q0TNZ8	9.98e-96	26	770	7	737	Penicillin-binding protein 1A OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbpA PE=3 SV=1
A7GHV1	1.77e-92	30	745	3	700	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
A7FY32	1.88e-92	30	745	3	700	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
A5I6G4	1.88e-92	30	745	3	700	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999954	0.000068	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
46	68