CAZyme Information: MGYG000000067_02206

Basic Information

• Species: Lachnoclostridium_B sp900066765
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium_B; Lachnoclostridium_B sp900066765
• CAZyme ID: MGYG000000067_02206
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1755	MGYG000000067_13|CGC1	193503.49	4.195

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000067	3710135	Isolate	United Kingdom	Europe

• Gene Location: Start: 38611; End: 43878 Strand: -

Full Sequence      

MKQQWKRAAAFFLSICLLFSVMPVTASAAVSDDVRISISDMTTEYMANPIGIETDSVHFG
WKLESNGIGTKQTAYQIQVTESGTSKAVWDSGKVENDKSVGISYGGEALAEGTAYDWTVK
VWTETGSEVQSGTASFETGVTNQQAWKNAAFIRMNKSSAAPVFRTEQKLDGKVESARLYI
TAIGAYQAYVNGSRAGVQEDGETVYHHMNPGYGNRNISMGYQAYDVTSMLEEQDTAVVSV
IGGTGWNDGNGGTNIGATSAQPAVKAMLRIRYADDTVKDIVTNTTDWKGTLDSGITANGV
YYGEDYDARIAEELGDFTKAGYDDSAWVNSLTEGNDTQTPVIHQEFAAQENVKYAQLVVE
EIGPGTKGDNENRLQMMELELKDSQGNNVVSGLAPKVSNTFTWGTQWTESNLTDGDYGID
SDRGYSSEQVASGQTSFTLETPITIEFAFDSAVSVESLDIYPRIKGEPISGNECVNYPKK
YSLKVSENGTDWTTVDTYQVDSLRNTVSYPETDSEGVYSEADFGEVTAQDVRVAVYAVGP
AVMEDNENRLQIMELELLDSAEKNVAAGVTPTISRNFESAPQWSAANLTDGDYGLKNNAG
FTTEILDYGNEVLDMEDNPITIDFHFNQAVTFSGMKLYARTDKESIYYGVCPNYAKIYSI
QTSSDGGKTWKDIDGASKLDAGTLKNTVLSGKTAMSTTTYSGEIRAQAATPGKIIDKFSK
EPVSCVLYTGQAANSSLAGGEVNVDKYYAYEEPEDALYAGKYEAVSEGENILGDGITLKK
GQTMIVNLGQNMTAIPEVEFAAERGTRLTMRFAEMLNDGSASGNGATQADGPKGSIYQKS
LRNARSAVKYTFAGDGKEHYQTTMSFFGYQYIEFTATDDVTIYSVKSKALSSITEQTGNI
ETNNANVNKLFSNTLYGQMSNYFTTSTDCPQRDERQAWSGDTQAFAQTAVYNFNSVAFLN
EIQDILSENTLIKGYTPSVFDDMNGYFSTWAAGWSDVEIIDPWVLYSQTGDTSVLEENWD
AMVHYMDYMKAHERGANQAPIDLGADRNYGDWLSFQGTSVEVISDYYYGYMAQLMAKIAG
VLGNTEKQAAYEQQFEDIKETFLKTHVTFNDGNLVIKSGTGDMQRYQFMWWAGKGGTWED
NSQTALLWMLKLGFYDSDEMRDAAKELLIENIKNENPAAGSIRANYGKNTLAVGFLGSNV
ITPVLSDIGSSDVSYDLLLQDEQPSWLFEVKAGATTIWERWNSYTPGVGFGDSEMNSFNH
YAYGSVVEWMYRYMAGIAADEENPGFKNIILQPTPDTGEKYNDEERIHSVDASYESYYGK
IESQWKSTDGELTSYHTVIPANTTAVLYLPVKENALDGFEALTGMSYEGMEEHNGLETAK
FVLESGGYDFSVKDGKLTAVYAEGYVGQEDGDDPVVVESITVTAPTKTEYTAGEELNLDG
MKVTAKYSDDTTKDIAVTDCKVSGYDKNKTGEQTVTVTYEGKIATFKVTVKEAAKPNDTD
KKKLEAAIKNAVPDTKKDHYSEASWAAYEEALKKAEEVFADTAATQKEIDDAAAALDEAA
KALQWKKLPYEDVVENDWFYDEVAYNYYEGTMTGMDDTHFAPYTTLVRAQFATILYRLNG
EPKVEYEAKFPDVPDGQFYSKAVTWAAETEVVTGYTDSGYFGTNDPITREQMVTMMYRYA
NYKKYKSEKPTDISAFTDADKVTEFAEDAMKWAVGNGIIAGKENEDGSYRLDPQGGTSRA
ECAIIIQRFMEKFEK

Enzyme Prediction     

EC	3.2.1.40	3.1.1.73

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	779	1349	3.8e-131	0.9900793650793651
CBM67	156	330	2.5e-19	0.8693181818181818

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	5.81e-92	895	1274	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	3.38e-30	171	327	1	147	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	9.81e-17	776	881	1	97	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	1.65e-16	1276	1358	1	72	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam07523	Big_3	3.93e-12	1425	1490	2	67	Bacterial Ig-like domain (group 3). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QXE01526.1	1.94e-175	12	1406	15	1190
AYA74416.1	3.64e-156	700	1393	350	1003
CBG75612.1	3.22e-142	700	1391	346	1017
QYX83505.1	8.65e-142	706	1350	354	976
ACX65306.1	1.81e-141	713	1391	343	1005

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	1.67e-74	776	1350	491	995	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6I60_A	1.41e-56	751	1350	331	899	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
6GSZ_A	3.90e-56	769	1352	314	849	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]
2L7Y_A	3.01e-08	1414	1498	3	89	Solutionstructure of a putative surface protein [Streptococcus pneumoniae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KNB2	1.90e-75	780	1393	367	923	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
Q82PP4	7.88e-74	776	1350	491	995	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
P9WF03	1.48e-61	783	1381	367	906	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	1.91e-31	47	327	41	344	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
P38535	5.15e-14	1566	1752	904	1083	Exoglucanase XynX OS=Acetivibrio thermocellus OX=1515 GN=xynX PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000258	0.998976	0.000188	0.000202	0.000180	0.000162

TMHMM  Annotations     

start	end
9	31