CAZyme Information: MGYG000000067_02212

Basic Information

• Species: Lachnoclostridium_B sp900066765
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium_B; Lachnoclostridium_B sp900066765
• CAZyme ID: MGYG000000067_02212
• CAZy Family: GH146
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1937		214610.13	4.2747

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000067	3710135	Isolate	United Kingdom	Europe

• Gene Location: Start: 47772; End: 53585 Strand: -

Full Sequence      

MKRRILAVLLAAATVLTSVPAYAAEPVEAMGENCVMQLEFEGNVADSIASERQTVVKKWS
GDESINAQEGTDYQFVEGIIGEKALQLNGGSYLSLGAEADLNPSNLTFSCWINPQEKMTG
EQILVWNKQLWNQDGWYLSSLNDEEPLVLSIGTRVYEVHMSGTRDEFFPAGVWTHIAVTF
DNETKKVTMYRNGESQFVSTGTTADQIVDNGAEKAIGYNGSEYKGSYLKARLDQVILLDT
AANADQIAKLYEEGKKEMTDAEIVVADKSILNPFSGLNTSAITADISLPEKGANGSTITW
ESTDKDVISETGKVVRQAEDTEVTLTAVIKSGDVEDTKSFTVTVKKKPQLTEEEQLWVSY
DMTVKDGKLVDSQGRGDAALVGLSAADVTTAENGEKQLNFTDNANHSKYVKLPQEIIEQG
DESFTIDMKFNTSQKAFAWIFNLGTKGTADYVFLNPIRAWGPTVLSVKSSVVSGDEHNVD
VGDVIEAGADTVATMVFNDNQTAQLYINGKLIGSVNHGCNITDILENGVTDPADAIGYLG
LSLYGADPGYEGTISRFDVYNYAMTEKEVALLYVDKYLQMADEERVAADVAELKKNLKEG
ELTASELELPDMGMNGSKIVWESSKKDVIAEDGAVTRPAIGKPDEAVTLTATVSRGEVSQ
KVTFDFNVLAVTEMQGMEDFELGTVDVTNSYYAQISQKDIDFLKTFDADRLLSRFRETAG
LDTKGVGPYTGWENSYIGGHTLGHYMTACAQAYLTADNAGDKAWMKTQLDTIISGLKACQ
DAVGTGFIFGAQIEDKNNIEKQFDIMEGTASGGNWVPWYTMHKILAGLVDVYKYTDNDEA
LAVASNLGDWIYNRVSKWDDSMKRRVLGIEYGGMNDCLYELYKYSGKAEHAKAAAQFDET
WLFESVLSGNANVLNGKHANTQIPKFVGALNRYRTLNGQMLDGEEVDAEIYLEYVEAFWD
MVVNKHTYITGGNSEWEHFGADNILDAERTQCNCETCNTYNMLKMTRELYKITGDKKYAD
YYENTFINAIISSINPETGMTTYFQPMATGYFKVYGNTDVNKNQFWCCTGSGMENFTKLG
DSIYYKKGKNLYVTQYLSSEAEWKEQNIKLTQTTSIPDKDTSKFTVHVLGGKSADMNLYL
RIPDWIAGEPTVTVNGKKEEVVVSGGYVCLDREWADGDTVEIQLPMEVRAYGLPDDETVF
GFKYGPVVLSAELGTEEMDKFSGCGASVMLPGSKIVNGSQAMPKDGKRAVLGTETLSIEE
TTAEDFIENINEYLVRVDDGDELRFELNGTDQKLVFSAHYRQHTQRYGIYWYFTGNDLDP
AEAEARILAQKEEGRTNQVKIDVTKAGYGQYEFDDLHQLWENGSQGTSSDNELNGMTSRY
AVADKDFSYRMAVNKEKDNYIVAKLAKIDNGKTLKITIEGQIIYEEVLNYSGDDAIYEVK
IPIPDEIVQKAQTVSVTEGDGNQKDYDVLRIYFSGKAGENSARLVEEVYIATGYSNHAEI
ESMQSDVGTTDFDAATKTYTVTVPEETEKAAITTKIADTYGLLYIDGKLVNDSIAQKFDV
KDEASVMTFRVYGEDHETYADYTVKVVRQKKTEEPEAEIVSLAVTAPEKTEYKKGENLDF
TGMKVTANYSDGSVKDIAVTDCEVSGYDKTKTGEQTVTVTYEGKTATFKVTVKEAEKPDT
TDKTALEAAVKAAIADTEKAKYTEESWAAYEEALNQAKEVLADADATQKEIDDAAAALDK
AAEALESKGLPYVDVVKDDWFYNAAYYNYFAGTMTGTDPTHFSPYATLVRAQFATILYRL
NGEPKVEYETKFPDVPDGQFYSKAVIWAAEAEVVTGYTDSGYFGTNDPITREQMVTMMYR
YAKHMEYESEEPTDISAFTDADKVTKFAEEAMKWAVANGIIAGKENEDGSYRLDPQGDTS
RAECAIIIQRFMEKFEK

Enzyme Prediction     

No EC number prediction in MGYG000000067_02212.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH146	685	1210	6.2e-177	0.9960238568588469

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	3.55e-133	685	1210	2	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	1.17e-98	673	1217	2	508	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam13385	Laminin_G_3	3.46e-16	87	248	1	151	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.
pfam07523	Big_3	3.82e-13	1607	1672	2	67	Bacterial Ig-like domain (group 3). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.
NF033190	inl_like_NEAT_1	4.26e-08	1811	1933	581	692	NEAT domain-containing leucine-rich repeat protein. Members of this family have an N-terminal NEAT (near transporter) domain often associated with iron transport, followed by a leucine-rich repeat region with significant sequence similarity to the internalins of Listeria monocytogenes. However, since Bacillus cereus (from which this protein was described, in PMID:16978259) is not considered an intracellular pathogen, and the function may be iron transport rather than internalization, applying the name "internalin" to this family probably would be misleading.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL17203.1	3.61e-249	677	1490	36	815
ANY68649.1	2.87e-236	36	1934	48	1657
ADL33431.1	3.63e-216	677	1492	2	779
AIQ16786.1	2.83e-213	677	1587	16	859
QSF44176.1	3.35e-212	677	1588	16	860

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6YQH_AAA	9.81e-98	680	1433	28	762	ChainAAA, Acetyl-CoA carboxylase, biotin carboxylase [Bacteroides thetaiotaomicron VPI-5482]
5OPJ_A	6.21e-94	680	1433	28	762	Beta-L-arabinofuranosidase[Bacteroides thetaiotaomicron]
6EX6_A	1.34e-24	818	1212	142	547	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
3WRE_A	1.63e-09	952	1212	294	568	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.65e-09	952	1212	294	568	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P19424	1.22e-13	1746	1934	35	216	Endoglucanase OS=Bacillus sp. (strain KSM-635) OX=1415 PE=1 SV=1
P38537	5.14e-09	1771	1936	53	210	Surface-layer 125 kDa protein OS=Lysinibacillus sphaericus OX=1421 PE=3 SV=1
E8MGH8	8.92e-09	952	1212	294	568	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1
C6CRV0	9.33e-09	1754	1930	1285	1457	Endo-1,4-beta-xylanase A OS=Paenibacillus sp. (strain JDR-2) OX=324057 GN=xynA1 PE=1 SV=1
A0A401ETL2	4.74e-08	1512	1683	1301	1470	Exo-beta-1,6-galactobiohydrolase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=bl1,6Gal PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000211	0.999108	0.000169	0.000173	0.000157	0.000141

TMHMM  Annotations     

start	end
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