CAZyme Information: MGYG000000071_00979

Basic Information

• Species: Pseudoflavonifractor capillosus
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; Pseudoflavonifractor; Pseudoflavonifractor capillosus
• CAZyme ID: MGYG000000071_00979
• CAZy Family: GH16
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
290		31213.95	3.6217

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000071	3913719	Isolate	United Kingdom	Europe

• Gene Location: Start: 128969; End: 129841 Strand: -

Full Sequence      

MKKNVPVLVLSAALALTLTACGETPSASGTPSGEPSAPAVTTPAATAPAATTPVVLDEEA
LGGDMLADFSNGPVDAMFASDGWSNGGVFNVWWTGSNVTYTDGQLHLGISEAGDEVEQDY
YGGEVRTGLYYGYGDYSVKMKPSKTEGTASTFFTCTGPYDIANGEPNPWDEIDIEFLGKD
TTGVQFNYYVDGQGGHEYWYELGFDASEEFHEYGFRWEEDSITWFVDGEAVYSVNTEDNG
PMPSTPGRILMNYWCGTEDAEGWMGVYTPDSATVDYEWVKTSAQGAELGA

Enzyme Prediction     

No EC number prediction in MGYG000000071_00979.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	69	280	8e-73	0.9854368932038835

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02175	GH16_lichenase	3.12e-103	65	283	1	212	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
pfam00722	Glyco_hydro_16	2.11e-44	93	276	1	166	Glycosyl hydrolases family 16.
COG2273	BglS	7.33e-32	78	279	60	262	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
cd00413	Glyco_hydrolase_16	4.74e-31	68	276	2	206	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
cd02176	GH16_XET	6.88e-20	89	263	10	175	Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADU22000.1	2.34e-76	14	283	13	261
EGC02853.1	1.50e-69	57	283	48	262
SNV04827.1	1.93e-69	68	280	50	255
BDA09400.1	5.48e-69	68	280	50	255
QIB59261.1	2.20e-68	68	280	50	255

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3I4I_A	3.46e-59	80	279	39	229	Crystalstructure of a prokaryotic beta-1,3-1,4-glucanase (lichenase) derived from a mouse hindgut metagenome [uncultured murine large bowel bacterium BAC 14],3I4I_B Crystal structure of a prokaryotic beta-1,3-1,4-glucanase (lichenase) derived from a mouse hindgut metagenome [uncultured murine large bowel bacterium BAC 14]
1BYH_A	9.55e-57	62	280	3	210	MOLECULARAND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-GLUCANASE [synthetic construct],1GLH_A Cation Binding To A Bacillus (1,3-1,4)-Beta-Glucanase. Geometry, Affinity And Effect On Protein Stability [Paenibacillus macerans],2AYH_A Crystal And Molecular Structure At 1.6 Angstroms Resolution Of The Hybrid Bacillus Endo-1,3-1,4-Beta-D-Glucan 4- Glucanohydrolase H(A16-M) [hybrid]
3WVJ_A	6.48e-56	66	285	9	217	ChainA, Beta-glucanase [Acetivibrio thermocellus ATCC 27405],3WVJ_B Chain B, Beta-glucanase [Acetivibrio thermocellus ATCC 27405]
1MAC_A	7.17e-56	80	280	19	208	CrystalStructure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]
1U0A_A	7.62e-56	62	280	3	210	ChainA, Beta-glucanase [Paenibacillus macerans],1U0A_B Chain B, Beta-glucanase [Paenibacillus macerans],1U0A_C Chain C, Beta-glucanase [Paenibacillus macerans],1U0A_D Chain D, Beta-glucanase [Paenibacillus macerans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P37073	1.79e-59	83	280	59	248	Beta-glucanase OS=Brevibacillus brevis OX=1393 GN=bglBB PE=1 SV=1
Q53317	1.22e-56	65	287	574	792	Xylanase/beta-glucanase OS=Ruminococcus flavefaciens OX=1265 GN=xynD PE=3 SV=2
P23904	8.38e-55	80	280	44	233	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2
O14412	2.12e-54	78	283	50	242	Beta-glucanase OS=Orpinomyces sp. (strain PC-2) OX=50059 GN=licA PE=1 SV=1
A3DBX3	9.10e-54	66	285	38	246	Beta-glucanase OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=licB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000000	1.000069	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000071_00979.