CAZyme Information: MGYG000000072_00572

Basic Information

• Species: UBA1394 sp900066845
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UBA1394; UBA1394 sp900066845
• CAZyme ID: MGYG000000072_00572
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
877		97160.92	4.3441

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000072	2648835	Isolate	United Kingdom	Europe

• Gene Location: Start: 271174; End: 273807 Strand: +

Full Sequence      

MADNKNNRKGSGNYRENTGRSKSGESVNRSGKNAGRRSPYAAYTGKDGTYKKPSGKTGGS
SHAGRTDSNNEQFKKESTAADNVRRTASQNARHTVRKKPENPKVKIAKKIAAVLGTTLLS
IFLVVVITGTIVATAMTVYVLEFMDESTDVTIKELENSANTIVYAQDGDKLVQLYAVTSE
VQRIPVDIEQIPQHVRDAFVCVEDERFYSHEGVDYKRTFAAFANMFMHIYDTKQGGSTIT
QQLIKNLTGDDDPSPERKIREIFRAMQFEKKYSKDEILENYLNYIGFGGPTNGIQLASIK
YFGKDVSELSIAEAACLAAIPKSPETINPFAGYEDENGKWVNTGKEANRERQESVLYHMY
GNGAISYDQYQEALNEKLIFTDSEEYKEAHPESDAEELMDEQKASSWVVDTALREYAAVL
MEEYGIDEEEAFKRINTGGYQIYTTVDLEMQKYVEEKYSDLNNLMDAESNSTWVDEDEDG
EYEQLYPESAFIAMDYNGNILSIAGGIGVKEKLLIFNRATMAKRQPGSCIKPITTYGLAL
YSDHIHWGSMYKDSPIKLEGEKWPENYDYVWRGYNMFIFEALRESRNTVPAQLCQELTPQ
AVFNFATQNLNVDLVDITEEGATDIAYAPLTVGALTYGISPQNLVNAYIPYGNGGTYCDA
HIVSRVERGDGSVVYENDGNPHEAIDPETAYVMNHLLQEVIKNGTGTAAQLSNKTVAGKT
GTSEDWNDLCFVGLTEDFVSGVWIGYDEKQELNHGLSSAQVWYNIIGEYANSIESDNSYP
ACDSVIEAPMCSSTGLIAGPYCSSSITGYWKSSNAPTCANHSSAPKKDEETPKEDESSED
SESSESSESSQDESSSVDSSSSDSSADNSSQDEPPSE

Enzyme Prediction     

No EC number prediction in MGYG000000072_00572.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	178	360	2.8e-59	0.9548022598870056

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	2.60e-150	114	771	11	598	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	1.01e-146	182	762	1	521	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	2.02e-113	108	817	3	765	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
pfam00912	Transgly	1.77e-65	176	360	6	177	Transglycosylase. The penicillin-binding proteins are bifunctional proteins consisting of transglycosylase and transpeptidase in the N- and C-terminus respectively. The transglycosylase domain catalyzes the polymerization of murein glycan chains.
COG4953	PbpC	2.48e-61	156	745	32	534	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL16716.1	2.29e-263	83	833	37	806
ADU22273.1	2.14e-162	89	821	3	739
CCO04758.1	2.20e-162	96	816	14	733
CBL33874.1	1.47e-137	97	818	27	754
BCI59848.1	6.33e-137	97	820	23	737

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2JE5_A	4.85e-51	142	825	23	693	StructuralAnd Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6],2JE5_B Structural And Mechanistic Basis Of Penicillin Binding Protein Inhibition By Lactivicins [Streptococcus pneumoniae R6]
4OON_A	1.13e-49	163	767	23	720	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
3ZG8_B	6.33e-47	267	769	2	455	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
3DWK_A	7.32e-47	186	751	30	562	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
7U4H_A	2.67e-45	183	817	40	808	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7GHV1	3.06e-82	161	769	57	643	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
A5I6G4	2.23e-81	161	769	57	643	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
A7FY32	2.23e-81	161	769	57	643	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
Q8XJ01	4.71e-76	122	787	32	679	Penicillin-binding protein 1A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=pbpA PE=3 SV=1
Q891X1	2.87e-75	153	802	53	690	Penicillin-binding protein 1A OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000078	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
111	133