CAZyme Information: MGYG000000073_00591

Basic Information

• Species: GCA-900066905 sp900066905
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; GCA-900066905; GCA-900066905; GCA-900066905 sp900066905
• CAZyme ID: MGYG000000073_00591
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
640	MGYG000000073_1|CGC10	72890.12	5.6173

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000073	2615710	Isolate	United Kingdom	Europe

• Gene Location: Start: 669226; End: 671148 Strand: +

Full Sequence      

MNRIHAVPMKHVNIQDGFISRAQRLMRESVIPYQWRALNDSVPGAEKSYCIQNLKIAAGK
IKGERGGMVFQDSDLAKWIEAAAYLLQSERDAQLEAHCDEIIDLMAQGQMEDGYFNTYFQ
LVEPQRRFKDLRDCHELYCCGHFIEAAVAYYEATGKRKLLEIMERMVALIAREFGPEEGQ
RRGYPGHQEIELALVKLYRTTGKKEYLDLAKFFIDERGREPYYFDLERQQLPDEHFPNSQ
YGREYFQAHLPVRQQQEVVGHSVRAMYMLCGMADVALETGDVTLYQACRRLWASTVQKRM
YITGAIGSTAYGEAFTFDYHLPNDTIYGETCAAIGLCFFASRMLQFGADREYADILERAL
YNGVLSGISLDGKRFFYVNPLEVWPQETREVHDFKEVKPQRQPWFGCACCPPNLARFISS
LGQYAYGYDAHNLYIHLYIGGTVDVPLGDGMLAALRVDSTLPYGGQISIEIRSPCHYGLA
LRVPGWCQEYTVCVNGEAVKAPVEDGYLYLHRDWMKGDKVTFALAMEPVRMYANAAIHHD
AGLVAIQYGPMVYCAEEADNGKDLHNLVLPSGAAIYAEYCENCLGGVMTLKTEGLRREGA
DDALYTAQPPRFIPQEIRLIPYFAWANRGEGEMRVWLQEK

Enzyme Prediction     

No EC number prediction in MGYG000000073_00591.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	12	554	4.4e-218	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	0.0	10	640	10	588	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	1.75e-166	11	554	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3629	DnrI	7.97e-04	19	221	35	225	DNA-binding transcriptional activator of the SARP family [Signal transduction mechanisms].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AGB41651.1	3.98e-245	4	639	3	645
ADH61394.1	2.92e-240	3	640	8	648
AZO96465.1	2.13e-236	4	639	8	651
AQS56927.1	2.06e-234	1	639	1	643
QJD87024.1	9.86e-233	6	640	9	653

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	1.61e-228	11	640	15	648	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.69e-220	11	640	15	648	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	3.52e-163	11	636	2	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	4.96e-163	11	636	2	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	1.40e-162	11	636	2	655	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.93e-162	11	636	2	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000088	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000073_00591.