CAZyme Information: MGYG000000076_00851

Basic Information

• Species: Roseburia intestinalis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Roseburia; Roseburia intestinalis
• CAZyme ID: MGYG000000076_00851
• CAZy Family: GH2
• CAZyme Description: Beta-glucuronidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
600	MGYG000000076_5|CGC5	68460.05	5.0872

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000076	4318260	Isolate	United Kingdom	Europe

• Gene Location: Start: 154154; End: 155956 Strand: +

Full Sequence      

MLYPQENKIRGIIDLNGVWDFALGTTEEPGDAVTLPEQMEPIAVPASYNDQKDDIAYRNH
YGYAYYRRNITVPSYYKGQRLVLRFDAVTHFAKIYLNGVLLTQHKGGFLPFEVDITDKLC
AGESAELVVAVDNRINHSTLPVGNEEGTSFMGADNVGVPGVEAAKRWRKPQNLPNFDFFN
YAGINRPVRIYTTPKAYIKDVTLVTDIRGTDGIVNYQVKTSDTDGQEVVLQILDANGNEV
AQAKGTSGEIVIPDAKLWEPYPGTPYLYTAAVTFGDDYYEEPFGVRTVRVEGTSFLINGK
PFYFKGFGKHEDSAFHGRGMDVCLDVKDVNLIHWLHANSFRTSHYPYAEEMYRLCDREGI
VIIDEVPAVGIGAGAGINPYETFPIREHHEQVIKDMIARDKNHPCVVMWSLGNEPDTENF
PESAYKYWHSLYELAHETDPSNRPVTLVCCQNNYEKDMVTRSMDVVCINRYYGWYNLSGD
LDAACYGLNLELDFWEKQNKPVMITEYGADAVAGIHECVPEMFSEEFQVEFYKRQNAQFD
KRKFFIGEHVWNFADFATVQGCMRVDGNKKGLFTRERRPKMAAHYFRERWGEVPDFGYKR

Enzyme Prediction     

EC	3.2.1.31

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	8	586	5.9e-110	0.6077127659574468

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	0.0	1	600	1	602	beta-D-glucuronidase; Provisional
COG3250	LacZ	3.32e-110	1	590	1	595	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam02836	Glyco_hydro_2_C	3.58e-80	288	593	1	300	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10340	ebgA	2.40e-36	66	568	113	556	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	9.34e-35	15	445	55	485	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL12719.1	0.0	1	600	1	600
CBL08206.1	0.0	1	599	1	599
VCV23245.1	0.0	1	599	1	599
AEN95939.1	0.0	1	599	6	605
CUO31707.1	0.0	1	599	1	600

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6U7I_A	2.71e-301	1	599	1	597	Faecalibacteriumprausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_B Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_C Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii],6U7I_D Faecalibacterium prausnitzii Beta-glucuronidase [Faecalibacterium prausnitzii]
6LD0_A	9.04e-253	1	599	3	669	ChainA, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD0_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LD6_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDB_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDC_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_A Chain A, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_B Chain B, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_C Chain C, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1],6LDD_D Chain D, LacZ1 Beta-galactosidase [Bifidobacterium dentium Bd1]
5Z1B_A	1.37e-252	1	599	25	691	Structureof Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_B Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_C Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679],5Z1B_D Structure of Bifidobacterium dentium beta-glucuronidase complexed with coumarin-3-O-glucuronide [Bifidobacterium dentium ATCC 27679]
6BO6_A	3.91e-217	1	599	1	609	Eubacteriumeligens beta-glucuronidase bound to UNC4917 glucuronic acid conjugate [[Eubacterium] eligens ATCC 27750]
6BJQ_A	8.61e-217	1	599	25	633	ChainA, Glycoside Hydrolase Family 2 candidate b-glucuronidase [[Eubacterium] eligens ATCC 27750]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P05804	1.64e-158	1	600	1	603	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2
Q4FAT7	3.91e-152	1	593	28	629	Beta-glucuronidase OS=Sus scrofa OX=9823 GN=GUSB PE=3 SV=1
O97524	7.60e-149	1	593	27	628	Beta-glucuronidase OS=Felis catus OX=9685 GN=GUSB PE=1 SV=1
P06760	4.91e-146	1	593	27	625	Beta-glucuronidase OS=Rattus norvegicus OX=10116 GN=Gusb PE=1 SV=1
Q5R5N6	1.52e-145	1	593	27	629	Beta-glucuronidase OS=Pongo abelii OX=9601 GN=GUSB PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000066	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000076_00851.