CAZyme Information: MGYG000000077_01140

Basic Information

• Species: Anaerobutyricum soehngenii
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Anaerobutyricum; Anaerobutyricum soehngenii
• CAZyme ID: MGYG000000077_01140
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
203	MGYG000000077_6|CGC1	24300.69	9.5479

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000077	3184592	Isolate	United Kingdom	Europe

• Gene Location: Start: 64616; End: 65227 Strand: +

Full Sequence      

MSKYYPDISHYHPVRNWDTVKRSCPFLIMKATQGISYIDKTLYTNIKECEKRGIPYWLYA
FLNRGKEVDQTRFLVEICRERIEKHFVGYVLDVESGNAEADIEKSLDFLKRQRHKMMLYT
MYSEYEMYKQVIYRRPKNCAWWEARYGLNNGKYNPHYDVHKGADLQQFTSNGFCPGISGR
VDLNRITDQGKSERWFRNSGKRN

Enzyme Prediction     

No EC number prediction in MGYG000000077_01140.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	7	177	1.6e-25	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06523	GH25_PlyB-like	1.50e-21	4	186	1	177	PlyB is a bacteriophage endolysin that displays potent lytic activity toward Bacillus anthracis. PlyB has an N-terminal glycosyl hydrolase family 25 (GH25) catalytic domain and a C-terminal bacterial SH3-like domain, SH3b. Both domains are required for effective catalytic activity. Endolysins are produced by bacteriophages at the end of their life cycle and participate in lysing the bacterial cell in order to release the newly formed progeny. Endolysins (also referred to as endo-N-acetylmuramidases or peptidoglycan hydrolases) degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
cd00599	GH25_muramidase	6.39e-20	7	184	4	184	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
cd06524	GH25_YegX-like	7.66e-15	7	185	4	191	YegX is an uncharacterized bacterial protein with a glycosyl hydrolase family 25 (GH25) catalytic domain that is similar in sequence to the CH-type (Chalaropsis-type) lysozymes of the GH25 family of endolysins.
pfam01183	Glyco_hydro_25	2.63e-14	7	177	2	180	Glycosyl hydrolases family 25.
cd06414	GH25_LytC-like	8.77e-13	7	184	5	188	The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SOB72115.1	1.66e-155	1	203	1	203
QUF81551.1	3.73e-118	1	201	1	201
QFG05701.1	3.68e-16	7	194	11	198
QLY79309.1	1.33e-14	7	188	6	187
BBN97864.1	5.87e-14	7	184	461	654

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000069	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000077_01140.