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CAZyme Information: MGYG000000080_01400

You are here: Home > Sequence: MGYG000000080_01400

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Anaerostipes caccae
Lineage Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Anaerostipes; Anaerostipes caccae
CAZyme ID MGYG000000080_01400
CAZy Family CBM48
CAZyme Description 1,4-alpha-glucan branching enzyme GlgB
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
766 MGYG000000080_2|CGC1 88181.12 6.9503
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000080 3256205 Isolate United Kingdom Europe
Gene Location Start: 341800;  End: 344100  Strand: +

Full Sequence      Download help

METNISKEDL  YLFQTGKAQK  AYLMFGCHYL  KEADAHEFVV  WAPNAKSVSL  VGDFNQWNPL60
SHPMKQTEPG  IYSIQVEGLE  KGDLYKYYVE  GMDGKCRYKS  DPFAFYSEMR  PDTASVVWDE120
LDGFSWRDKR  YLSRRKKKQD  VSQPMSVYEM  HLGTWKMPKE  EEREFYNYRE  IADMLVPYLQ180
KMGYTHVELM  PVTEYPYDLS  WGYQVTGYYS  VTSRYGTPED  FMYFIDKLHR  ADIGVILDWV240
PAHFPRDEHG  LALFDGSHIY  DHADPRKGSQ  PDWGTLLFNY  GKPEVQSFLI  SSAVFFAEKY300
HIDGIRIDAV  SAMLYLDFGK  KPGEFVPNED  GTNINYEAVD  FLKNLNRTMR  SNFEGFITVA360
EESTAFPKVT  SAVDDEEGLG  FTYKWNMGYM  HDSLYYMELD  SLFRKENHGA  IVFSMDYAYS420
ENYILPYSHD  EVVHGKGSMI  NKMFGEYEQK  FGSLKTLYGF  MFAHPGKKLL  FMGDDFAQFV480
EWRDKEELDW  FLIDEYETHK  TMNDYVAKLN  ELYCSEPALY  ELDNKPEGFE  WLLQRDADHS540
IVAFIRKSKK  RRGKPQEQIV  CIFNFTPVEW  DKYHIPMPKD  GKLTKILDTG  EKAYGGSGEV600
SQKSAKVRKK  KIKGKRNAYE  HYAVITLKPL  SAVMYRYTLE  DTPAKKKAEP  KKTEAKKPSP660
KAAGTAKAEA  VKTPVKKEVS  VVTEPASAAE  TTLKTAEVQA  KKETPKAAGP  AAGKKAEKPE720
AKKEQTTKDI  QKPKKKEAEK  ETKQQTEDST  KKKTAAKSAD  KKEETK766

Enzyme Prediction      help

EC 2.4.1.18

CAZyme Signature Domains help

Created with Snap3876114153191229268306344383421459497536574612651689727177478GH1325105CBM48
Family Start End Evalue family coverage
GH13 177 478 8.2e-144 0.9966777408637874
CBM48 25 105 2.3e-17 0.868421052631579

CDD Domains      download full data without filtering help

Created with Snap38761141531912292683063443834214594975365746126516897271637PRK123139604PRK14705109513AmyAc_Glg_BE24598PRK147065636PRK05402
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
PRK12313 PRK12313 0.0 1 637 3 627
1,4-alpha-glucan branching protein GlgB.
PRK14705 PRK14705 0.0 9 604 607 1193
glycogen branching enzyme; Provisional
cd11322 AmyAc_Glg_BE 0.0 109 513 1 400
Alpha amylase catalytic domain found in the Glycogen branching enzyme (also called 1,4-alpha-glucan branching enzyme). The glycogen branching enzyme catalyzes the third step of glycogen biosynthesis by the cleavage of an alpha-(1,4)-glucosidic linkage and the formation a new alpha-(1,6)-branch by subsequent transfer of cleaved oligosaccharide. They are part of a group called branching enzymes which catalyze the formation of alpha-1,6 branch points in either glycogen or starch. This group includes proteins from bacteria, eukaryotes, and archaea. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
PRK14706 PRK14706 0.0 24 598 26 587
glycogen branching enzyme; Provisional
PRK05402 PRK05402 0.0 5 636 100 722
1,4-alpha-glucan branching protein GlgB.

CAZyme Hits      help

Created with Snap38761141531912292683063443834214594975365746126516897271702QMW70941.1|CBM48|GH13_91702BCD36168.1|CBM48|GH13_91662QCP35773.1|CBM48|GH13_91657AQP40138.1|CBM48|GH13_91652CBL37815.1|CBM48|GH13_9
Hit ID E-Value Query Start Query End Hit Start Hit End
QMW70941.1 0.0 1 702 1 702
BCD36168.1 0.0 1 702 1 702
QCP35773.1 0.0 1 662 1 662
AQP40138.1 0.0 1 657 1 658
CBL37815.1 0.0 1 652 1 657

PDB Hits      download full data without filtering help

Created with Snap387611415319122926830634438342145949753657461265168972755975GQW_A55975GR5_A55975GR2_A55975GQZ_A55975GQU_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
5GQW_A 9.76e-196 5 597 129 737
Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A 1.38e-195 5 597 129 737
Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR2_A 1.38e-195 5 597 129 737
Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQZ_A 1.95e-195 5 597 129 737
Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A 2.75e-195 5 597 129 737
Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits      download full data without filtering help

Created with Snap38761141531912292683063443834214594975365746126516897275642sp|P30539|GLGB_BUTFI9597sp|Q8DLB8|GLGB_THEVB5637sp|O66936|GLGB_AQUAE5598sp|Q1AZ86|GLGB_RUBXD4602sp|Q8CZE8|GLGB_OCEIH
Hit ID E-Value Query Start Query End Hit Start Hit End Description
P30539 1.44e-207 5 642 7 637
1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q8DLB8 1.53e-204 9 597 110 714
1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
O66936 4.42e-204 5 637 7 628
1,4-alpha-glucan branching enzyme GlgB OS=Aquifex aeolicus (strain VF5) OX=224324 GN=glgB PE=3 SV=1
Q1AZ86 5.88e-204 5 598 99 685
1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
Q8CZE8 1.59e-203 4 602 3 596
1,4-alpha-glucan branching enzyme GlgB OS=Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831) OX=221109 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000087 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000000080_01400.