CAZyme Information: MGYG000000082_02806

Basic Information

• Species: Acinetobacter mesopotamicus
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Moraxellaceae; Acinetobacter; Acinetobacter mesopotamicus
• CAZyme ID: MGYG000000082_02806
• CAZy Family: CE4
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
611		70400.34	9.0911

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000082	3378595	Isolate	United Kingdom	Europe

• Gene Location: Start: 14764; End: 16599 Strand: +

Full Sequence      

MKKFTKILMALSLVGTVYVPMLSHAELQKPRQIKLQDNQFVTLTFHDVRDDVAKRGDRDV
YAISTKNLGQYLEWIQREGWHPIRLEDAWLARQGKKTLPNKALLLTFDDGALSSYNRVFP
LLKQYKTPAVFAIPTSWINGNTKDAYEAYGKGNLMTWPQMREMVASGYAEFVSHSDNMHR
GILANPQQNMQPAAITREYIEKQKRYETESEYQSRVVVDLKKSKQTLDRELGINSRAIFW
PYGAVTTETEELAAQAGLPMSFSLGSVAPLADSVKTYQRALIMDNPTPEMINQEMLEFLN
YVRAPYKQRKSFLRFDLAEMQSANNEQFNQKLGQLLDKVDGLKTSTIILKAVSDSNGDGK
IDVAYFPNRQLKMQQDMLNRVVWQSRTRISNRVYAELPLSLETEQKLDLSVLTADLVKNN
TSIEGLMLDTGSVLDCAVRQQKWDTTCEEKVQQVFQIKERTKDQAKYYANISNNYQTALK
FKLDQTQLVGLKPLLDYTLDYADFIYVELDPIQQPKVFNAFLEQLKTFDDKEQQYLIASF
DVPEQLTKQQVKQLKKNYQQLRSLGIQKVGVNNYSFNQAQSIHQNFYPELSLNSSPLSYR
DPFTQKVLEKK

Enzyme Prediction     

No EC number prediction in MGYG000000082_02806.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CE4	95	261	1.7e-24	0.9384615384615385

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR03938	deacetyl_PgaB	7.40e-139	38	467	1	413	poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB. Two well-characterized systems produce polysaccharide based on N-acetyl-D-glucosamine in straight chains with beta-1,6 linkages. These are encoded by the icaADBC operon in Staphylococcus species, where the system is designated polysaccharide intercellular adhesin (PIA), and the pgaABCD operon in Gram-negative bacteria such as E. coli. Both systems include a putative polysaccharide deacetylase. The PgaB protein, described here, contains an additional domain lacking from its Gram-positive counterpart IcaB (TIGR03933). Deacetylation by this protein appears necessary to allow export through the porin PgaA [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]
PRK14582	pgaB	3.38e-87	7	407	15	412	poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB.
cd10964	CE4_PgaB_5s	9.96e-77	98	285	1	192	N-terminal putative catalytic polysaccharide deacetylase domain of bacterial poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB, and similar proteins. This family is represented by an outer membrane lipoprotein, poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (PgaB, EC 3.5.1.-), encoded by Escherichia coli pgaB gene from the pgaABCD (formerly ycdSRQP) operon, which affects biofilm development by promoting abiotic surface binding and intercellular adhesion. PgaB catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide that stabilizes biofilms of E. coli and other bacteria. PgaB contains an N-terminal NodB homology domain with a 5-stranded beta/alpha barrel, and a C-terminal carbohydrate binding domain required for PGA N-deacetylation, which may be involved in binding to unmodified poly-beta-1,6-GlcNAc and assisting catalysis by the deacetylase domain. This family also includes several orthologs of PgaB, such as the hemin storage system HmsF protein, encoded by Yersinia pestis hmsF gene from the hmsHFRS operon, which is essential for Y. pestis biofilm formation. Like PgaB, HmsF is an outer membrane protein with an N-terminal NodB homology domain, which is likely involved in the modification of the exopolysaccharide (EPS) component of the biofilm. HmsF also has a conserved but uncharacterized C-terminal domain that is present in other HmsF-like proteins in Gram-negative bacteria. This alignment model corresponds to the N-terminal NodB homology domain.
PRK14581	hmsF	1.58e-75	8	424	15	428	outer membrane N-deacetylase; Provisional
cd10918	CE4_NodB_like_5s_6s	2.81e-29	102	263	1	134	Putative catalytic NodB homology domain of PgaB, IcaB, and similar proteins which consist of a deformed (beta/alpha)8 barrel fold with 5- or 6-strands. This family belongs to the large and functionally diverse carbohydrate esterase 4 (CE4) superfamily, whose members show strong sequence similarity with some variability due to their distinct carbohydrate substrates. It includes bacterial poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase PgaB, hemin storage system HmsF protein in gram-negative species, intercellular adhesion proteins IcaB, and many uncharacterized prokaryotic polysaccharide deacetylases. It also includes a putative polysaccharide deacetylase YxkH encoded by the Bacillus subtilis yxkH gene, which is one of six polysaccharide deacetylase gene homologs present in the Bacillus subtilis genome. Sequence comparison shows all family members contain a conserved domain similar to the catalytic NodB homology domain of rhizobial NodB-like proteins, which consists of a deformed (beta/alpha)8 barrel fold with 6 or 7 strands. However, in this family, most proteins have 5 strands and some have 6 strands. Moreover, long insertions are found in many family members, whose function remains unknown.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QPF34271.1	0.0	1	611	1	611
AUC05455.1	0.0	1	611	1	611
QPF37289.1	0.0	1	611	1	611
AYA02587.1	3.77e-270	1	605	1	614
QXW25272.1	8.84e-260	36	605	49	618

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4F9D_A	4.49e-58	38	399	10	366	Structureof Escherichia coli PgaB 42-655 in complex with nickel [Escherichia coli K-12],4F9D_B Structure of Escherichia coli PgaB 42-655 in complex with nickel [Escherichia coli K-12]
4F9J_A	5.45e-55	38	399	10	366	Structureof Escherichia coli PgaB 42-655 in complex with iron [Escherichia coli K-12],4F9J_B Structure of Escherichia coli PgaB 42-655 in complex with iron [Escherichia coli K-12]
5BU6_A	6.26e-54	40	284	13	264	Structureof BpsB deaceylase domain from Bordetella bronchiseptica [Bordetella bronchiseptica RB50],5BU6_B Structure of BpsB deaceylase domain from Bordetella bronchiseptica [Bordetella bronchiseptica RB50]
4U10_A	1.70e-42	38	265	2	233	Probingthe structure and mechanism of de-N-acetylase from aggregatibacter actinomycetemcomitans [Aggregatibacter actinomycetemcomitans],4U10_B Probing the structure and mechanism of de-N-acetylase from aggregatibacter actinomycetemcomitans [Aggregatibacter actinomycetemcomitans]
3VUS_A	3.15e-41	38	296	6	265	Escherichiacoli PgaB N-terminal domain [Escherichia coli K-12],3VUS_B Escherichia coli PgaB N-terminal domain [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8XAR3	4.28e-57	38	399	47	403	Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase OS=Escherichia coli O157:H7 OX=83334 GN=pgaB PE=3 SV=1
P75906	5.91e-57	38	399	47	403	Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase OS=Escherichia coli (strain K12) OX=83333 GN=pgaB PE=1 SV=1
Q5HKP8	2.58e-13	4	293	8	281	Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=icaB PE=3 SV=1
Q6TYB1	2.58e-13	4	293	8	281	Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase OS=Staphylococcus epidermidis OX=1282 GN=icaB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000257	0.999013	0.000215	0.000169	0.000163	0.000150

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000082_02806.