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CAZyme Information: MGYG000000093_03517

You are here: Home > Sequence: MGYG000000093_03517

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Klebsiella_A grimontii
Lineage Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella_A; Klebsiella_A grimontii
CAZyme ID MGYG000000093_03517
CAZy Family CBM41
CAZyme Description Pullulanase
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
1090 MGYG000000093_6|CGC1 118113.28 4.4251
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000093 6212272 Isolate United Kingdom Europe
Gene Location Start: 58881;  End: 62153  Strand: -

Full Sequence      Download help

Enzyme Prediction      help

No EC number prediction in MGYG000000093_03517.

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH13 469 888 3.4e-143 0.997229916897507
CBM41 75 171 3.2e-18 0.8921568627450981

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
TIGR02103 pullul_strch 0.0 180 1077 1 898
alpha-1,6-glucosidases, pullulanase-type. Members of this protein family include secreted (or membrane-anchored) pullulanases of Gram-negative bacteria and pullulanase-type starch debranching enzymes of plants. Both enzymes hydrolyze alpha-1,6 glycosidic linkages. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. This family is closely homologous to, but architecturally different from, the Gram-positive pullulanases of Gram-positive bacteria (TIGR02102). [Energy metabolism, Biosynthesis and degradation of polysaccharides]
PLN02877 PLN02877 0.0 162 1072 71 965
alpha-amylase/limit dextrinase
cd11341 AmyAc_Pullulanase_LD-like 8.97e-167 431 919 3 395
Alpha amylase catalytic domain found in Pullulanase (also called dextrinase; alpha-dextrin endo-1,6-alpha glucosidase), limit dextrinase, and related proteins. Pullulanase is an enzyme with action similar to that of isoamylase; it cleaves 1,6-alpha-glucosidic linkages in pullulan, amylopectin, and glycogen, and in alpha-and beta-amylase limit-dextrins of amylopectin and glycogen. Pullulanases are very similar to limit dextrinases, although they differ in their action on glycogen and the rate of hydrolysis of limit dextrins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.
TIGR02104 pulA_typeI 2.40e-124 314 1030 22 585
pullulanase, type I. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate. This family consists of pullulanases related to the subfamilies described in TIGR02102 and TIGR02103 but having a different domain architecture with shorter sequences. Members are called type I pullulanases.
COG1523 PulA 9.94e-90 312 1073 68 689
Pullulanase/glycogen debranching enzyme [Carbohydrate transport and metabolism].

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
QLT90062.1 0.0 1 1090 1 1090
QQQ21107.1 0.0 1 1090 1 1090
AWT19361.1 0.0 1 1090 1 1090
CAA36431.1 0.0 1 1090 1 1090
QXW38709.1 0.0 1 1090 1 1090

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
5YN2_A 0.0 1 1090 1 1102
ChainA, PulA protein [Klebsiella pneumoniae],5YN7_A Chain A, PulA protein [Klebsiella pneumoniae],5YNA_A Chain A, PulA protein [Klebsiella pneumoniae],5YNC_A Chain A, PulA protein [Klebsiella pneumoniae],5YND_A Chain A, DUF3372 domain-containing protein [Klebsiella pneumoniae],5YNE_A Chain A, PulA protein [Klebsiella pneumoniae],5YNH_A Chain A, PulA protein [Klebsiella pneumoniae]
2YOC_A 0.0 21 1089 2 1070
ChainA, Pullulanase [Klebsiella oxytoca],2YOC_B Chain B, Pullulanase [Klebsiella oxytoca]
6J35_A 0.0 39 1090 2 1053
ChainA, Pullulanase [Klebsiella pneumoniae],6J35_B Chain B, Pullulanase [Klebsiella pneumoniae],6J4H_A Chain A, Pullulanase [Klebsiella pneumoniae]
6J33_A 0.0 39 1090 2 1053
ChainA, pullulanase [Klebsiella pneumoniae],6J33_B Chain B, pullulanase [Klebsiella pneumoniae],6J34_A Chain A, Pullulanase [Klebsiella pneumoniae]
2FGZ_A 0.0 36 1090 29 1083
ChainA, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FH6_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FH8_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FHB_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FHC_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae],2FHF_A Chain A, Alpha-dextrin endo-1,6-alpha-glucosidase [Klebsiella pneumoniae]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
P07206 0.0 1 1090 1 1090
Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2
P07811 0.0 1 1077 1 1087
Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1
Q8GTR4 3.63e-166 184 1072 88 958
Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2
O33840 8.51e-57 79 983 34 765
Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2
C0SPA0 2.58e-52 285 914 91 597
Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as LIPO

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
0.000000 0.000000 1.000072 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000000093_03517.