CAZyme Information: MGYG000000100_00964

Basic Information

• Species: CABIZH01 sp902363205
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Selenomonadales; Massilibacillaceae; CABIZH01; CABIZH01 sp902363205
• CAZyme ID: MGYG000000100_00964
• CAZy Family: GT51
• CAZyme Description: Penicillin-binding protein 2D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
650		71999.41	6.2199

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000100	2685351	Isolate	Canada	North America

• Gene Location: Start: 27303; End: 29255 Strand: +

Full Sequence      

MLKIFITMIFLALGSFAYVFNGLPDAQDMNQLKLMSASQVYDIRGELIAKLFEENRVVVP
LNKMSPYLIDAIIANEDTRFYQHRGIDPIGIARALVVNITSGDIAEGGSTITQQLARGMF
LNQDRTLLRKIREAILAIKLDVRFSKEEILHAYLNQIYLGEGAYGVEAASQAYFGKSAGD
LDLSESAMIAGLAKGPIYYSPYNNIERAKERRDTVINGMYLQGYITEAMKEDATQEVLKI
KSKRERSVKASYFLDYVAKELVDLYGEEKVYTGGLKIYTTLDMHLQKIAEQTLKEYQGAV
VAINPSDGYIYAMVGGNNYSESQLNRVTDEYRQPGSAFKPIVYATAINKGMKTNTVMVDE
PIDIAGYKPLNYDNKYRGAMTLKKALRWSVNTVAVKIGQQVGMHDVIQLANNLGLSTITK
ADEHLATVLGGVSVGVNLLDLSTAYTAFANGGILSKPIAVLEVRNEHDQVLDTFTTQQSA
VLSPETAYIMTDMLMGTIYNGTASGANIGREAAGKTGTTDNYETAWFIGYIPDLLVGIYV
GNDNRVPTDLSGTQVAEMWGQFMKQAISNKQKRDFSVPDTIIKGIDICANTGELAESNCK
DHEYSAFIKGTEPKRFADKIIEKILPKEEKGSGKEKKNKWWFNFPGLPKL

Enzyme Prediction     

No EC number prediction in MGYG000000100_00964.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	45	220	4.6e-70	0.9887005649717514

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0744	MrcB	0.0	1	569	20	599	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
TIGR02074	PBP_1a_fam	0.0	55	569	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG5009	MrcA	0.0	1	615	8	766	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
PRK11636	mrcA	2.26e-108	9	617	15	832	penicillin-binding protein 1a; Provisional
COG4953	PbpC	1.02e-103	1	618	4	607	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QJW44953.1	2.74e-268	2	648	6	657
QJW44930.1	2.74e-268	2	648	6	657
QDR80647.1	7.28e-268	3	613	4	618
BBB93057.1	6.93e-261	6	647	14	657
AJQ27600.1	1.62e-254	23	648	27	655

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U2G_A	2.78e-81	21	615	5	802	2.6Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20],5U2G_B 2.6 Angstrom Resolution Crystal Structure of Penicillin-Binding Protein 1A from Haemophilus influenzae [Haemophilus influenzae Rd KW20]
3UDF_A	1.05e-80	34	567	17	716	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]
7U4H_A	8.39e-76	25	634	9	826	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
4OON_A	1.10e-70	29	582	12	738	Crystalstructure of PBP1a in complex with compound 17 ((4Z,8S,11E,14S)-5-(2-amino-1,3-thiazol-4-yl)-14-(5,6-dihydroxy-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-8-formyl-2-methyl-6-oxo-3,10-dioxa-4,7,11-triazatetradeca-4,11-diene-2,12,14-tricarboxylic acid) [Pseudomonas aeruginosa PAO1]
5FGZ_A	2.26e-66	54	556	154	682	E.coli PBP1b in complex with FPI-1465 [Escherichia coli K-12],5HL9_A E. coli PBP1b in complex with acyl-ampicillin and moenomycin [Escherichia coli K-12],5HLA_A E. coli PBP1b in complex with acyl-cephalexin and moenomycin [Escherichia coli K-12],5HLB_A E. coli PBP1b in complex with acyl-aztreonam and moenomycin [Escherichia coli K-12],5HLD_A E. coli PBP1b in complex with acyl-CENTA and moenomycin [Escherichia coli K-12],6YN0_A Structure of E. coli PBP1b with a FtsN peptide activating transglycosylase activity [Escherichia coli K-12],7LQ6_A Chain A, Penicillin-binding protein 1B [Escherichia coli K-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O66874	1.71e-119	19	613	26	712	Penicillin-binding protein 1A OS=Aquifex aeolicus (strain VF5) OX=224324 GN=mrcA PE=1 SV=1
P38050	9.25e-101	3	543	8	571	Penicillin-binding protein 1F OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpF PE=2 SV=2
P70997	1.45e-100	4	646	33	688	Penicillin-binding protein 2D OS=Bacillus subtilis (strain 168) OX=224308 GN=pbpG PE=2 SV=3
Q9KNU5	2.77e-93	6	625	19	815	Penicillin-binding protein 1A OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mrcA PE=3 SV=2
Q92G78	3.80e-92	2	594	9	743	Penicillin-binding protein 1A OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=mrcA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.832905	0.155185	0.010872	0.000252	0.000183	0.000608

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000100_00964.