CAZyme Information: MGYG000000104_01854

Basic Information

• Species: Clostridium cuniculi
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium cuniculi
• CAZyme ID: MGYG000000104_01854
• CAZy Family: GH84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2143	MGYG000000104_11|CGC1	239665.19	4.1909

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000104	3679160	Isolate	Canada	North America

• Gene Location: Start: 14371; End: 20802 Strand: +

Full Sequence      

MRREMLKRLLATTMASVFIINGLTITKVDAFTDKNNEVSISLPKLNPVPENIEVNEGILT
ITSTVNLQGEDLADEDAIRVLKEFLSENNIVINESYDESSTTLIIGEVEDDIQEMDEAKE
RIGVSGVEELKDEGYVLAVDSSDGNNGTILIEGKDEDGTFYGVKTLTQLVTNTGSKVYSN
DVLITDEPTMKTRGIVEGFYGTPWTHEERLDQIRFYGENKMNTYIYAPKDDPYHRDNWRE
PYPESEMARMNELIQTSNENKVDFVFAISPGIDIRFDGEEGEEDFKALVSKCESLYDMGV
RSFAILFDDISNKDGVKQAELLNRFNEEFIKVKGDVKPLITVPTEYDTHAMGGSIDEVSQ
YTRDFSSTLSSDIMVMWTGPVVVSEGIDLENANFVNSVYGDRMGIWWNYPVTDYMKEKLA
LGPIYNADKALEGNIDFFTMNPMEHAELSKITLATGADYSWNTSEYDYQESWKKAIEMMY
GDLAPYMITFANHSSRMEASWASTGRGDAPEVRQTMDDLLRKIVKGQDVTTEIEILNKEF
NDMIEAADVLKEKLPDNVLSHCSENLDKLKLLGENDKLALDYLIAKRDKNTEEIERLEAL
LNDNLSSLNSGKKLSEKTALAFITEALESDFNAKANFDVSSTFVAPGEEVKFTNLSSMAS
SEFEWTFKGANIESSTEENPTVVYEKEGIYSVTLKAKNTLGNDVIVKEGLITVSNSANTE
KTNLALGKVVTASSSVAASEAAEMAVDGKTNTKWCANKGNTHTLTIDLGKVDTITDVVIK
HSEAGGEAASMNTKAYRIEVSTDGENYKEIINVTNNTAGITTDKIPVTKARYVKLIVEKP
TQGSDNAARIYEVEVMGVEGDIELPPIYVVPGEEKEPIVYPVPQETTYLSDEGMELVGEV
NVVIHGDQEEATVGKLQEILKENNIKYNISEEVANDKANIVLTSDKNHCDNCVDSDLLED
EALNNKEGYVIKTSNDENEKGNISIVASDSEGAYYGVLTLGQILEKESNNKIVEVVISDY
PEIEFRGFIEGFYGTPWSHEDRISLMKDTSEYKMNTYIYAPKDDPYHRSNWKDLYPEEEA
KQIQELAQAGTENNINFCWTIHPGATLKFTEDDFDALIAKYEQLYDLGVRQFGVLFDDTD
DWTNGKKQAEWINRIDTEFVKAKGDVAPMIVISARYNSAWGPNMDRYFKPFMETLHSDIQ
VMWTGHATMSNISKDVMEWPKTQTGVDKDLAVWWNYPVNDYCDSRILMAPLHNLSTDLNN
VSGFFSNPMNQAEASKVALYSIADYTWNTDEFDYMKSWETSIEKLVPEIKEEFMRFASNT
CYLKDDGGASGAFEYDESWYLAEKIDALKSAISNNEPLVDKANDLLTEFNIIVSDYDVIK
ENINNDNLLSEIEPFLNSYKALGEAGVAAMKSLIASEEGKIEEWLDNSNLANEKLELMNT
FTVDRLENDTITPYTVAVGEKRLKPIINEVITVSRDIISKTIFKEIKPEVISSQKDLNID
VKFENGEYSIGEITALELNKDEYIGIALPKANKLSEVIINASDYEGLVLEYSLNGIEWSE
AETNITDGVMKSNNSIAATFVRIVNKGDNKKTIDINSFEAKPVYKMTPTISENIGTYQSY
NIDKALDGDRSTKYWSDKATSAGHYIQIDLGNKVPLYDVSAYFGATDYMRNSEYLISEDG
INWTSLGELQYTSEGDKKVATINANGQMARYIRIQANGDNSGYWVQLFEVEVNKTVPDAG
DNYVELVTGDLEGRVDYLYDSDLSTAFEAEINKTTQSLVYKMTRVTNVGNILFLQDKDNI
SKATVSVKNTNGVWKKVGILGKQLSDIEVNDDILEVKIEFDINNPSPKIYEIIVNENTEV
DEEVFKDHLQIAIDMANEITDEELENVVPIVVEEFKESLAKAIEVLNNSNSNQEEVNAAF
DRLAAAMQKLSFYKGDKEQLINLRDRISKLNKDEFIPSTWNNLESVLGEVNIIIDDENAL
ENEVKEGYNKLIKAYLELRLKPNKDKLQDLINKAETLDSSKYTEDSFSNVVNALEMAKLV
LANEEATEEEVHNAKGALELALGRLIENSTNKPEDNNSSNDNNSSSNNNNEGNNSSSGNS
NNTTNGSTNKGDLPKTGGSSAGVIGVFGAIISAIGASLFKKRK

Enzyme Prediction     

No EC number prediction in MGYG000000104_01854.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH84	193	491	1.1e-110	0.9932203389830508
GH84	1026	1317	2.3e-107	0.9932203389830508
CBM32	731	853	1.7e-25	0.9274193548387096
CBM32	1616	1730	5.3e-17	0.8870967741935484

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07555	NAGidase	2.72e-142	193	490	1	293	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam07555	NAGidase	2.96e-141	1026	1316	1	293	beta-N-acetylglucosaminidase. This family has previously been described as a hyaluronidase. However, more recently it has been shown that this family has beta-N-acetylglucosaminidase activity.
pfam00754	F5_F8_type_C	8.64e-18	745	849	17	124	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam02838	Glyco_hydro_20b	1.26e-14	47	186	6	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
COG3291	COG3291	4.89e-14	635	837	83	286	PKD repeat [Function unknown].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATD57955.1	0.0	872	2143	40	1316
SLK13973.1	0.0	872	2143	40	1316
QBJ74644.1	0.0	872	2143	40	1316
ATD54361.1	0.0	872	2143	40	1316
AYE33733.1	0.0	872	2143	40	1314

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2CBI_A	8.87e-216	32	630	5	590	Structureof the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase [Clostridium perfringens],2CBI_B Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase [Clostridium perfringens],2CBJ_A Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase in complex with PUGNAc [Clostridium perfringens],2CBJ_B Structure of the Clostridium perfringens NagJ family 84 glycoside hydrolase, a homologue of human O-GlcNAcase in complex with PUGNAc [Clostridium perfringens],2V5C_A Family 84 glycoside hydrolase from Clostridium perfringens, 2.1 Angstrom structure [Clostridium perfringens],2V5C_B Family 84 glycoside hydrolase from Clostridium perfringens, 2.1 Angstrom structure [Clostridium perfringens],2VUR_A Chemical dissection of the link between Streptozotocin, O-GlcNAc and pancreatic cell death [Clostridium perfringens],2VUR_B Chemical dissection of the link between Streptozotocin, O-GlcNAc and pancreatic cell death [Clostridium perfringens],2X0Y_A Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds [Clostridium perfringens],2X0Y_B Screening-based discovery of drug-like O-GlcNAcase inhibitor scaffolds [Clostridium perfringens]
2J62_A	1.24e-215	32	630	5	590	Structureof a bacterial O-glcnacase in complex with glcnacstatin [Clostridium perfringens],2J62_B Structure of a bacterial O-glcnacase in complex with glcnacstatin [Clostridium perfringens],2WB5_A GlcNAcstatins are nanomolar inhibitors of human O-GlcNAcase inducing cellular hyper-O-GlcNAcylation [Clostridium perfringens],2WB5_B GlcNAcstatins are nanomolar inhibitors of human O-GlcNAcase inducing cellular hyper-O-GlcNAcylation [Clostridium perfringens]
5OXD_A	4.07e-215	32	627	7	589	Complexof a C. perfringens O-GlcNAcase with a fragment hit [Clostridium perfringens]
2XPK_A	6.53e-215	32	630	5	590	Cell-penetrant,nanomolar O-GlcNAcase inhibitors selective against lysosomal hexosaminidases [Clostridium perfringens],2XPK_B Cell-penetrant, nanomolar O-GlcNAcase inhibitors selective against lysosomal hexosaminidases [Clostridium perfringens]
2YDQ_A	2.14e-214	32	627	7	589	CpOGAD298N in complex with hOGA-derived O-GlcNAc peptide [Clostridium perfringens],2YDR_A CpOGA D298N in complex with p53-derived O-GlcNAc peptide [Clostridium perfringens],2YDS_A CpOGA D298N in complex with TAB1-derived O-GlcNAc peptide [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q0TR53	1.91e-218	1	692	1	682	O-GlcNAcase NagJ OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=nagJ PE=1 SV=1
Q8XL08	2.66e-218	1	692	1	682	O-GlcNAcase NagJ OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagJ PE=1 SV=1
Q89ZI2	1.76e-95	45	550	26	503	O-GlcNAcase BT_4395 OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_4395 PE=1 SV=1
P26831	2.53e-70	879	1732	41	943	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2
Q8VIJ5	8.85e-36	194	459	63	331	Protein O-GlcNAcase OS=Rattus norvegicus OX=10116 GN=Oga PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000317	0.998928	0.000212	0.000200	0.000174	0.000148

TMHMM  Annotations     

start	end
2117	2139