CAZyme Information: MGYG000000104_02392

Basic Information

• Species: Clostridium cuniculi
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium cuniculi
• CAZyme ID: MGYG000000104_02392
• CAZy Family: GH29
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1331	MGYG000000104_16|CGC1	146634.68	4.1737

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000104	3679160	Isolate	Canada	North America

• Gene Location: Start: 29847; End: 33842 Strand: -

Full Sequence      

MKNVRKLVASVVIGSIVCQMMPVKVIAETINSIMPPEAFGAIPNQSQLNYHEEELAAFIH
FGMNTFTNSEWGNGKENPNNFNPTDLDADEWVRTLKEAGFKRIIMIGKHHDGFVLWKSEV
TDHDVEKSTDWQATKGGEGDVLAEVSAACTKYDMDMGLYLSPWDANAPSYGYGEGTNDET
DTNGDYNEFYMAQLREVLGNPKYGNNGKFVEVWMDGAKGSGAAAQHYEFDKWFDLIEELQ
PGAVVFSPYGTDVRWIGNESGKAGDPVWSKINKQRIRDRYDQGLGDDNQYLNNGDPQGDI
WSVGECDVSLTSGWFWHQGNGPKTIEQLAEIYFKSVGRGQPLLLNVAPDKTGHFTAEDIA
RIKEFSNAINNTFDENLATPDTTTAEASSVRGNSMKFSATNVLDDNDDTYWTMDDGETTG
SITIDLGEEKIFDVISIEEYIKLGQRVSEFSVDVFTNGVWRNFGRGKTIGAKRLVRNSAV
SASKIRINIEDSLAVPLIENVEVYKADEAFEVEALTPAGTDFIDNVDFDNKDRWIQEDIG
IGNTGMYSSNSGTNASFKFTGTKAWIVGTFDPSHGIMEVWIDDEKVDEVDTYKPERSVSQ
IIYSTDDLEYGEHTVKIVVKGEKNSSSRGNAIGLDCAYYLNNNGAGMFEIEEDNYTVNEG
DTKEIKIKRVGGTTGLATVHFSTSPDSAVHGRHYNDVNEIIEFVDGQETATISISTVDNN
EKAGNVKFFCNIDTPTNGAIIGFNKKSEVTIIDNDIDRPYTEENPFFLPGTFGEEKLLEA
EDLQLGGTGGEIESKDGASDGKVVGWLGNTSRGNAWLNMWVNSEEASAYDMEIRYLAGAN
DILYYTNNDGSILGEIQCENTSPNFGTKTIRVDLNKGLDKIKFFNDDASTVNIDSIKITK
VGVDKSDLVKVIENAKNIIENETDKYTESSIDALKEAIAIAEETVANEVATQEQVEVAKI
AVENAISRLEEKVEKPENVNKLALKISIDYANELKESGALDEVVPAVVQEFNKVLEEAIS
IYNNELATQDEVDTIFNNLVEVIHMLEFNQGDKSELENLINSANALNKEDYTEESWSNLE
VILQEANKVFADENSMQDEVNEVVDKLQQAIKELEEEKKVDKSILQGLVDKVKETDSSKY
IPSTWTKFENALEAANSILVNEDVTQEEVDSSYNALLRAYLSLRLTPDKSLLEGLIKEVS
SIDLSKYTAKSAEIVEKALEDANKVLNNEEATEKEVNTALENLKNAKNSLVASSDSNSNT
NSNQNSNSDSNANSNSGNSSTNNNKDSSNSSTGNSTTGKLPQTGTTGVAGTLISGIVTLL
CGLGLSRKKNN

Enzyme Prediction     

No EC number prediction in MGYG000000104_02392.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH29	44	367	8.7e-67	0.8554913294797688

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3669	AfuC	2.94e-87	40	504	8	430	Alpha-L-fucosidase [Carbohydrate transport and metabolism].
pfam01120	Alpha_L_fucos	5.42e-28	81	364	79	323	Alpha-L-fucosidase.
smart00812	Alpha_L_fucos	9.17e-28	50	372	23	340	Alpha-L-fucosidase. O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in 'clans'. Family 29 encompasses alpha-L-fucosidases, which is a lysosomal enzyme responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Deficiency of alpha-L-fucosidase results in the lysosomal storage disease fucosidosis.
smart00237	Calx_beta	5.08e-10	645	729	1	86	Domains in Na-Ca exchangers and integrin-beta4. Domain in Na-Ca exchangers and integrin subunit beta4 (and some cyanobacterial proteins)
pfam03160	Calx-beta	7.08e-09	645	733	1	91	Calx-beta domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SLK21779.1	3.95e-216	20	756	23	745
ATD55836.1	3.95e-216	20	756	23	745
QBJ76136.1	3.95e-216	20	756	23	745
ATD56491.1	3.95e-216	20	756	23	745
AYE34854.1	4.31e-215	38	756	41	745

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6ORG_A	1.31e-148	40	505	8	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORG_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6OR4_A	1.86e-147	40	505	8	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6OR4_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_A Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORH_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6ORF_A	1.93e-147	40	505	8	449	Crystalstructure of SpGH29 [Streptococcus pneumoniae TIGR4],6ORF_B Crystal structure of SpGH29 [Streptococcus pneumoniae TIGR4]
6TR3_A	1.14e-133	36	532	21	531	Ruminococcusgnavus GH29 fucosidase E1_10125 in complex with fucose [[Ruminococcus] gnavus E1]
6TR4_A	1.63e-132	36	532	21	531	Ruminococcusgnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1],6TR4_B Ruminococcus gnavus GH29 fucosidase E1_10125 D221A mutant in complex with fucose [[Ruminococcus] gnavus E1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8GW72	2.21e-107	42	504	37	477	Alpha-L-fucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=FUC1 PE=1 SV=2
Q7XUR3	1.43e-103	35	504	32	476	Putative alpha-L-fucosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0560400 PE=3 SV=2
E8MGH9	2.17e-11	1052	1246	1667	1871	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1
P10901	3.34e-10	50	254	82	262	Alpha-L-fucosidase OS=Dictyostelium discoideum OX=44689 GN=alfA PE=3 SV=1
Q9L7Q2	9.45e-10	977	1176	414	617	Zinc metalloprotease ZmpB OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=zmpB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000614	0.941557	0.057077	0.000259	0.000248	0.000221

TMHMM  Annotations     

start	end
1303	1325