CAZyme Information: MGYG000000104_02910

Basic Information

• Species: Clostridium cuniculi
• Lineage: Bacteria; Firmicutes_A; Clostridia; Clostridiales; Clostridiaceae; Clostridium; Clostridium cuniculi
• CAZyme ID: MGYG000000104_02910
• CAZy Family: CBM51
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1867		210902.39	4.3293

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000104	3679160	Isolate	Canada	North America

• Gene Location: Start: 11784; End: 17387 Strand: +

Full Sequence      

MLRKKLSSIIVVALITNLASTPLNVFAETTNINKIIEDSQETQETNKAEVSKFTPYYSQY
KDAYDKEFKMDNSNIESITSTGGNLRENVGTGNIIDGNLDTYWETGRHTSNSFTNELIFT
LKEETVLNRIAYRSAWNTVGFAEDFEIWASDAAEGNDFNLVASATTTKTADVIEIKFNET
NFKRIKFVFKNNGTATASEMMFYKKDSVLDKMNSIFTDSTLSAVSEEFNTIEEINKLEEE
AKSHPLYEEYKENFENARALLQQNKIESSEATISKFDTYYSEYKDAYDKVFKMDNSNIAS
ITSTGGNLRDSVGTANIIDGNLDTYWETGKHTSDSFTNELIFTLKEDTVLNRIAYRSAWN
TVGFAEDFEIWASSTTKGDTFQLVTSATASKTADVVEIKFNPTSFKRIKFVFKNTGTATT
SEMMFYKEDAAQDKLKGLFADDTLTKVSEEFNSIEALNELEESVKEHPLYKEFKQSIEDA
KVIVENKQIEATKATMRKATYVENEEYNNLYRMSYDNIKSISNNGGHYWNQVIGNAIDGD
LSSYWETKTGNTDIFNNEVEVTFNESVNLNRIVYAPRQSDLKGFAEEVEIYASRTSQGDT
YQLVATGTYNATSGFIEAKFDETEFKRVKFVFKSSTQNWASLSEIMFYESDSVWNSVNEL
FTDGTMSIVSDKFNTLDKINELEEVARKHPLYEEEFKSTIDLAKEIVNNPRQEDVIELEM
RGNSVQETNKRKMWGFQDWQVTGLSALAGDKITVYVDVEEGEPTPTLLYRQAMTQHGGAK
TFQLQNGKNEIIIPEIDATSNGILDGTIQGGELFFTNYNSDEQTRAPKVRIEGAKEYPVF
VLGESDENEVIKELEAYVEKIKNEPETTPDIFAVSSNKSLSLTQATYALEWYKNNNKTPK
YTAEGWDEIVEKAMDFWGYDNSSELNSDFNFRIMPMVKNLTGGAFMNAHSGVIGIRPGNQ
NCIVGADMGWGTMHELGHNFDTSGRTIAEVTNNIMPLYFESLNRTQTRITDQNIWENNTY
PKVGLDDYSNNELYNTSDSTHLAQLAPLWQLYLYDNTFYGKFEQQFRANDYGNKTREDIY
KSWVVAASDAMQLDLTEFFARHGIRVSEEVASEISSKYEKPDKKIYYLNDLAMNYEGNGF
TDNAQVDVKTTNSDGNVKLVFSINEEDKGNLLGYEIRKDGKYIGFTSKDSFVDTSSNLDD
DAVYTVTPYDIKLNTLEKIEVRALQPTISVNPVITIELGEEFNPKEYIVARDMKGNSIAD
SVKVESNVNTSRTGEYQVVYYVEGSGGIKYSATTKVNVVANKTYLSDITATTTQNGWGTV
RKDKSINGNAITLRRGEDVITYKKGLGLHSVSEYIYDLEGKDYEYFESYVGADTNSNSNL
TSVEFKVYVDGVEKYNSGIMTRSTEQKYVKVDVKNAKELKLVITDAGNGTDADHGNWADA
KLFSMESKPEIIGDNLVYSMDEEIDLMNGIIAKDYEDGDLTSKVEIKSSDFVEGKSGVFT
IVYTVTDSDGLTSEFSRFIAVTEEEVQLSSLNWESATIGSGVVRKNRAVSNNAIRLLDEN
KNVETFDTGIGTHSYSEIVYNSEGYDIFDTWVGVDQHVSERSESSVVFKVYVDGELKAQT
DVMKSDTPKERLVVDIRNSSEVKLVVDVADNGNTWDHADWANARFLKVADYDTTELEAVL
EKAKGIDLTLYTEETANELKEAMNKGESALTSKDQSVIDEAINVIEKAIERLVEKVDFSS
LEEVIANNSDLNELHYYKDAITSHKALVEEAKTILANENSTQEEIDTIIAKINESYKNLV
VRENKVELEKKLEEAKSIENKGYQEVRWENFLYGIDYASNIYNNQDATDDEIKSALFTLD
YFKSELK

Enzyme Prediction     

No EC number prediction in MGYG000000104_02910.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CBM51	1303	1442	1.6e-43	0.9925373134328358
CBM51	1527	1665	7e-39	0.9850746268656716

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam08305	NPCBM	3.99e-49	1303	1442	3	135	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)
pfam08305	NPCBM	2.48e-47	1525	1665	2	135	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)
smart00776	NPCBM	1.50e-45	1302	1442	4	144	This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins.
smart00776	NPCBM	6.19e-44	1522	1665	1	144	This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins.
pfam13402	Peptidase_M60	1.69e-16	833	1099	1	264	Peptidase M60, enhancin and enhancin-like. This family of peptidases contains a zinc metallopeptidase motif (HEXXHX(8,28)E) and possesses mucinase activity. It includes the viral enhancins as well as enhancin-like peptidases from bacterial species. Enhancins are a class of metalloproteases found in some baculoviruses that enhance viral infection by degrading the peritrophic membrane (PM) of the insect midgut. Bacterial enhancins are found to be cytotoxic when compared to viral enhancin, however, suggesting that the bacterial enhancins do not enhance infection in the same way as viral enhancin. Bacterial enhancins may have evolved a distinct biochemical function. These bacterial domains are peptidases targetting host glycoproteins and thus probably play an important role in successful colonisation of both vertebrate mucosal surfaces and the invertebrate digestive tract by both mutualistic and pathogenic microbes. This family has been augmented by a merge with the sequences in the Enhancin Pfam family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AQW28385.1	0.0	3	1867	3	1868
AFV15061.1	0.0	3	1867	3	1868
AEP95031.1	0.0	3	1867	3	1868
AWS27153.1	0.0	3	1867	3	1868
ATD50182.1	0.0	3	1867	3	1868

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6XSZ_A	0.0	715	1221	24	530	ChainA, ZmpC Glycopeptidase [Clostridium perfringens],6XSZ_B Chain B, ZmpC Glycopeptidase [Clostridium perfringens],6XSZ_C Chain C, ZmpC Glycopeptidase [Clostridium perfringens],6XSZ_D Chain D, ZmpC Glycopeptidase [Clostridium perfringens],6XT1_A Chain A, ZmpC Glycopeptidase [Clostridium perfringens],6XT1_B Chain B, ZmpC Glycopeptidase [Clostridium perfringens],6XT1_C Chain C, ZmpC Glycopeptidase [Clostridium perfringens],6XT1_D Chain D, ZmpC Glycopeptidase [Clostridium perfringens]
7JS4_A	0.0	715	1670	24	980	ChainA, F5/8 type C domain protein [Clostridium perfringens ATCC 13124]
7JFS_A	0.0	268	1221	24	980	ChainA, F5/8 type C domain protein [Clostridium perfringens ATCC 13124]
5KDJ_A	0.0	650	1302	23	674	ZmpBmetallopeptidase from Clostridium perfringens [Clostridium perfringens ATCC 13124],5KDJ_B ZmpB metallopeptidase from Clostridium perfringens [Clostridium perfringens ATCC 13124]
5KDN_A	3.60e-277	715	1221	24	529	ZmpBmetallopeptidase from Clostridium perfringens [Clostridium perfringens ATCC 13124],5KDS_A ZmpB metallopeptidase in complex with an O-glycopeptide (a2,6-sialylated core-3 pentapeptide). [Clostridium perfringens ATCC 13124],5KDU_A ZmpB metallopeptidase in complex with a2,6-Sialyl T-antigen [Clostridium perfringens ATCC 13124]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P56957	1.52e-10	1225	1532	260	590	Pesticidal crystal protein Cry22Aa OS=Bacillus thuringiensis OX=1428 GN=cry22Aa PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000291	0.998997	0.000187	0.000190	0.000159	0.000139

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000104_02910.