CAZyme Information: MGYG000000106_01107

Basic Information

• Species: Enterococcus_D gallinarum
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus_D; Enterococcus_D gallinarum
• CAZyme ID: MGYG000000106_01107
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
739		83495.06	7.6274

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000106	3151412	Isolate	Canada	North America

• Gene Location: Start: 134206; End: 136425 Strand: -

Full Sequence      

MSKQTKYFVYICALLPLLFLGNTGTVLAIEIESSSQTLTTETMNETSASNTSTTDSTSTV
SSDAEGASNTSSSEKSSQESTKEETQASDESQQETQEEPEKIYDVQGGLDPSSRNRSDLK
EQVGIQRRSLRSADVAFIEAGDPSTPSQDFIDVSSHNGIISVEQYKIIKSYGISSVVVKL
TEATSYRNPLAASQIANAQAAGLVVHAYHYSWFTTEAQAKAEAEYFVKYAKELKLPTSAL
MVNDIEDNQIMGKADHTALSKVFANRIKELGYSNTHHYTGVLWVTQKAIDPAALGYSNVW
IANYPYVPAGQKFTEYSAWQWTYNLSFPGVSGTFDMSSDYQKYFTGQGQGGAIPDNRYAT
ITKDNYTFWQNFSWKKKADSKDYLGQTLQIKYRYEHINGSTYFSVYDNQGTWIGYINAGG
TKTTDGAQGSWQSKETYVKFRSTGTVYRNFNFDSTAIVSEEMLRNLYKVTGTYRHFNGVV
YDSVYDLNGKWIGYIDSAKLSVENQGPAISDERYTTVTSANYSIWQNFSWKMRASSSSYL
HQTLKIKYRYEHLNGSVYFSLYDNQDNWVGYINANATKVADNAAGAVLFSGQYGTVTSKN
YSIWQNFSWKEKNKSANVYGQTLQVKRYYNHFNGSTYYSLYDNRGTWQGYINSGGMKMAD
NAAGSAQSYHKKIKFTQNFPLWQNFNWKKKSTVTDAMKQETYEARYVYRHFNGSNYLSIY
DNKGIWQGYINESATRQVN

Enzyme Prediction     

No EC number prediction in MGYG000000106_01107.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	151	330	2.3e-37	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06522	GH25_AtlA-like	1.21e-70	148	340	1	192	AtlA is an autolysin found in Gram-positive lactic acid bacteria that degrades bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family includes the AtlA and Aml autolysins from Streptococcus mutans which have a C-terminal glycosyl hydrolase family 25 (GH25) catalytic domain as well as six tandem N-terminal repeats of the GBS (group B Streptococcus) Bsp-like peptidoglycan-binding domain. Other members of this family have one or more C-terminal peptidoglycan-binding domain(s) (SH3 or LysM) in addition to the GH25 domain.
pfam01183	Glyco_hydro_25	2.88e-37	151	330	1	180	Glycosyl hydrolases family 25.
cd00599	GH25_muramidase	1.33e-28	150	337	2	184	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
cd06414	GH25_LytC-like	1.44e-19	151	337	4	188	The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.
cd06415	GH25_Cpl1-like	1.44e-16	150	336	3	195	Cpl-1 lysin (also known as Cpl-9 lysozyme / muramidase) is a bacterial cell wall endolysin encoded by the pneumococcal bacteriophage Cp-1, which cleaves the glycosidic N-acetylmuramoyl-(beta1,4)-N-acetylglucosamine bonds of the pneumococcal glycan chain, thus acting as an enzymatic antimicrobial agent (an enzybiotic) against streptococcal infections. Cpl-1 belongs to the CP family of lysozymes (CPL lysozymes) which includes the Cpl-7 lysin. Cpl-1 has a glycosyl hydrolase family 25 (GH25) catalytic domain with an irregular (beta/alpha)5-beta3 barrel and a C-terminal cell wall-anchoring module formed by six similar choline-binding repeats (ChBr's). The ChBr's facilitate the anchoring of Cpl-1 to the choline-containing teichoic acid of the pneumococcal cell wall. Other members of this domain family have an N-terminal CHAP (cysteine, histidine-dependent amidohydrolases/peptidases) domain similar to that of the firmicute CHAP lysins and associated with endopeptidase activity. The Cpl-7 lysin is also included here as is LysB of Lactococcus phage, and the Mur lysin of Lactobacillus phage.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QOG26641.1	0.0	1	739	1	739
QGR80690.1	0.0	1	739	1	739
AYY10113.1	0.0	1	737	1	738
EEV40600.1	0.0	100	738	107	744
AUJ84783.1	0.0	100	738	107	744

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5A6S_A	4.44e-10	152	347	26	212	Crystalstructure of the CTP1L endolysin reveals how its activity is regulated by a secondary translation product [Clostridium phage phiCTP1]
1H09_A	1.90e-07	150	361	7	225	ChainA, LYSOZYME [Streptococcus phage Cp1]
2IXU_A	1.91e-07	150	361	8	226	ChainA, LYSOZYME [Streptococcus phage Cp1]
2IXV_A	4.48e-07	150	361	8	226	ChainA, LYSOZYME [Streptococcus phage Cp1],2J8F_A Chain A, LYSOZYME [Streptococcus phage Cp1],2J8G_A Chain A, LYSOZYME [Streptococcus phage Cp1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P19385	6.26e-09	150	318	8	178	Lysozyme OS=Streptococcus phage Cp-7 OX=10748 GN=CPL7 PE=1 SV=2
P19386	2.53e-07	150	361	8	226	Lysozyme OS=Streptococcus phage Cp-9 OX=10749 GN=CPL9 PE=3 SV=1
P15057	1.05e-06	150	361	8	226	Lysozyme OS=Streptococcus phage Cp-1 OX=10747 GN=CPL1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000601	0.998717	0.000193	0.000170	0.000155	0.000148

TMHMM  Annotations     

start	end
7	29