CAZyme Information: MGYG000000110_02621

Basic Information

• Species: Kluyvera sp902363335
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Kluyvera; Kluyvera sp902363335
• CAZyme ID: MGYG000000110_02621
• CAZy Family: GH63
• CAZyme Description: Glucosidase YgjK

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
783	MGYG000000110_4|CGC4	87620.39	6.3522

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000110	4775474	Isolate	Canada	North America

• Gene Location: Start: 235623; End: 237974 Strand: -

Full Sequence      

MKIITTLTPLACVLLLSFSAHAVTADQFKNVINRTGAPQSMQDFDYDDHQRFNPFFDLGA
WHGHLLPDGPATMGGFPGPALLTEEYINFMATNFDRLTVYQNGKKVAFTMQAWSLPGALV
QTLSAPGVQVEMTLRFASAHTSLLETKITSDTPLKLVWDGELLEKLTSKEGKLLTTKTID
EAFPSYQRHLSATRDGLTVSFGKVRSDSDLMTSGESQYQVHRSLPTATKIDGHRFTSSAQ
INGSTTIYTTYSHLLTAEQVRLEKAQIRDILARPAHYLSASEQRWNGYLQKGLTNPDATA
EQTRVAVKAIETLNGNWRAPGGAVQHNTVTPSVTGRWFSGNQTWPWDTWKQAYAMAHFNP
EIAKENIRAVFSWQIAANDKVRPQDAGFVPDLIAWNLSPERGGDGSNWNERNTKPSLAAW
SVMEVYNVTKDKAWLAEMYPKLVAYHDWWLRNRDHNGNGVPEYGATRDKAHNTADGAMLF
TVKKDDKEETLSGLKTYEQVTAKGEYDSLEIPAQVAASWESGRDDAAVFGFIDKEQLDKY
LANGGKRSDWTVKFAENRDQSGNLLGYSLLQESVDQASYMYSDNHYLAEMATLLGKPEEA
KRYQALAKKLADYINTCMFDPQTKFYYDIRIEDQPLANGCAGKPIVERGKGPEGWSPLFN
GAATQAHADEVISVMLDNKEFNTFVPLGTAALTNPAFGADIYWRGRVWVDQLWFGLKGME
RYGHREEALKMADAFFKHAKGLTSDGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNEFF
RKP

Enzyme Prediction     

No EC number prediction in MGYG000000110_02621.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH63	4	780	7.7e-253	0.9912280701754386

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10137	PRK10137	0.0	1	782	1	786	alpha-glucosidase; Provisional
COG3408	GDB1	3.48e-06	337	770	281	597	Glycogen debranching enzyme (alpha-1,6-glucosidase) [Carbohydrate transport and metabolism].
pfam17389	Bac_rhamnosid6H	4.51e-06	579	628	174	223	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
PLN02567	PLN02567	3.96e-04	415	675	205	431	alpha,alpha-trehalase

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLO35000.1	0.0	1	783	1	783
QLT74513.1	0.0	1	783	1	783
QBG06353.1	0.0	1	783	1	783
AKL38765.1	0.0	1	783	1	783
AHW86850.1	0.0	1	783	1	783

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3D3I_A	0.0	25	782	3	760	Crystalstructural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63 [Escherichia coli K-12],3D3I_B Crystal structural of Escherichia coli K12 YgjK, a glucosidase belonging to glycoside hydrolase family 63 [Escherichia coli K-12]
3W7S_A	0.0	25	782	2	759	Escherichiacoli K12 YgjK complexed with glucose [Escherichia coli K-12],3W7S_B Escherichia coli K12 YgjK complexed with glucose [Escherichia coli K-12],3W7T_A Escherichia coli K12 YgjK complexed with mannose [Escherichia coli K-12],3W7T_B Escherichia coli K12 YgjK complexed with mannose [Escherichia coli K-12],3W7U_A Escherichia coli K12 YgjK complexed with galactose [Escherichia coli K-12],3W7U_B Escherichia coli K12 YgjK complexed with galactose [Escherichia coli K-12]
3W7W_A	0.0	25	782	2	759	Crystalstructure of E. coli YgjK E727A complexed with 2-O-alpha-D-glucopyranosyl-alpha-D-galactopyranose [Escherichia coli K-12],3W7W_B Crystal structure of E. coli YgjK E727A complexed with 2-O-alpha-D-glucopyranosyl-alpha-D-galactopyranose [Escherichia coli K-12],5GW7_A Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12],5GW7_B Crystal structure of the glycosynthase mutant E727A of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12]
3W7X_A	0.0	25	782	2	759	Crystalstructure of E. coli YgjK D324N complexed with melibiose [Escherichia coli K-12],3W7X_B Crystal structure of E. coli YgjK D324N complexed with melibiose [Escherichia coli K-12],5CA3_A Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12],5CA3_B Crystal structure of the glycosynthase mutant D324N of Escherichia coli GH63 glycosidase in complex with glucose and lactose [Escherichia coli K-12]
7PQQ_B	1.45e-280	25	484	328	787	ChainB, Anti-RON nanobody,Megabody 91,Glucosidase YgjK [Lama glama]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P42592	0.0	1	782	1	782	Glucosidase YgjK OS=Escherichia coli (strain K12) OX=83333 GN=ygjK PE=1 SV=1
D8QTR2	1.62e-13	344	770	98	479	Mannosylglycerate hydrolase MGH1 OS=Selaginella moellendorffii OX=88036 GN=MGH PE=1 SV=1
D8T3S4	2.85e-13	344	770	98	479	Mannosylglycerate hydrolase MGH2 OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_447962 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000247	0.998980	0.000240	0.000180	0.000169	0.000159

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000110_02621.