CAZyme Information: MGYG000000112_01252

Basic Information

• Species: Actinomyces sp900323545
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; Actinomyces sp900323545
• CAZyme ID: MGYG000000112_01252
• CAZy Family: GT4
• CAZyme Description: Glycosyltransferase Gtf1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
387	MGYG000000112_2|CGC1	39710.46	9.452

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000112	3112947	Isolate	Canada	North America

• Gene Location: Start: 12069; End: 13232 Strand: +

Full Sequence      

MSTSAVSGSNEAGTPLVPGTGRLRILQVSGSAAGGVRAHLADCARILASDGHDVIVEAPA
SVLDGLDVAPARAEPLEIGPRPSLGDTLAVARLRRLGRRAHAVHAHGLRAGALAALALGR
RRRGRTRLVVTLHNLTVGGRLTTLVGERLERLIASRADLVLAVSPDLAERARDLGAPRVE
LAVIPAAAPQSRSEPASPAPAALEDVWPQDSARVLTVARLAPQKGLPLLLDAAAILARDV
EAGHLPAFTWAVAGDGPGKAEAAERIAAERLPVSLLGRRTDATGLMEDADVVVQTSLWEG
QPLTIQEALRAGVAIVATDVGGTAVTARGGAVLVAPEAHAIAEALAAILTDPRARTRAAQ
SARQASGLLPGSEDLSAQLRQAVLAPH

Enzyme Prediction     

No EC number prediction in MGYG000000112_01252.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT4	211	357	6e-23	0.925

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03801	GT4_PimA-like	8.68e-23	24	336	1	317	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
cd03811	GT4_GT28_WabH-like	8.80e-21	149	334	128	310	family 4 and family 28 glycosyltransferases similar to Klebsiella WabH. This family is most closely related to the GT1 family of glycosyltransferases. WabH in Klebsiella pneumoniae has been shown to transfer a GlcNAc residue from UDP-GlcNAc onto the acceptor GalUA residue in the cellular outer core.
COG0438	RfaB	1.34e-18	22	336	2	326	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
cd03808	GT4_CapM-like	2.05e-17	148	323	131	298	capsular polysaccharide biosynthesis glycosyltransferase CapM and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. CapM in Staphylococcus aureus is required for the synthesis of type 1 capsular polysaccharides.
cd03819	GT4_WavL-like	5.63e-17	34	366	11	336	Vibrio cholerae WavL and similar sequences. This family is most closely related to the GT4 family of glycosyltransferases. WavL in Vibrio cholerae has been shown to be involved in the biosynthesis of the lipopolysaccharide core.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQQ58367.1	3.86e-183	4	387	2	385
QQC40440.1	1.10e-182	4	387	2	385
AMD99717.1	3.16e-182	4	387	2	385
QQC19995.1	9.35e-182	1	385	1	384
VEI15105.1	2.77e-181	1	385	1	385

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999996	0.000047	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000112_01252.