CAZyme Information: MGYG000000113_01828

Basic Information

• Species: Streptococcus salivarius
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus; Streptococcus salivarius
• CAZyme ID: MGYG000000113_01828
• CAZy Family: GH66
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1248		131905.27	4.2149

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000113	2251845	Isolate	Canada	North America

• Gene Location: Start: 39514; End: 43260 Strand: -

Full Sequence      

MSKKVASTKLLSGLFVAGGVLGMNQVAKADNVVSSEATKPVITTEADNLVVVPTEAVAPV
ATTEIGPSTATVATDTATTATASTIFSQAVPAESASSETLVASEALAPESAAVETITSSS
DNATEAGRHSTAQVTPVTEVTEQNLNGDAYLTDPETTKAAYSKTDGDINYSVVVSNPTAE
TKTMTVNLTLQHASEIIGQDNVDLTLAAGASAKVSNLTVASEWLTNNTGYLVTISVNDKS
GNVLSSKRAGLSVEDDWTVFPRYGIVAGSPTDQNSILVKNLEAYRKELELMKSMNINSYF
FYDAYNEATDPFPEGVDSFVQKWNTWSHTQVDTKAVKELVDQVHKSGAVAMLYNMISADS
NPKNPALPLAALAYNFYDSFGKKGEPMTYTIGDNPTQVYYDPANPDWQKYIAGVMKSAMD
RMGFDGWQGDTIGDNRVTDYEHRNSTDEADSHMMSDSYASFINAMKDLIGEKYYITINDV
NGGNDDKLAKARQDVVYNELWTNGGSVIPGRMQVAYGDLKARIDMVRNKTGKSLIVGAYM
EEPGIDYTVPGGKATNGAGKDALAGKPLQADATLLVDATVAAAGGYHMSIAALANANAAL
NVLQSAYYPTQYLSVAKDTIRKLYNYQQFITAYENLLRGEGVTNSTQAVSTKNASGEILS
KDALGVTGDQVWTFAKSGKGFSTVQMINMMGINAGWHNEEGYADNKTPDAQENLTVRLSL
AGKTAQEAAKIADQVYVTSPDDWATSSMKKAQASLETDENGQPVLVISVPKLTLWNMLYI
KEDTTATPVEPVTNQAGKKVDNTVTSEASSETAKSENTTVNKGSEAPTDTKPSVEAPKLD
ETTKPAPSVDELVNSAAVPVAIAVSETAHDKKDDNSVSNTDQGTVASDSITTPASEATST
AASTASSEVSESAIVSSEASETENSSEASTSESEIPTTTAISESHAVVEPVASLTESESQ
ASTSLVSETTSTIVSVAPSEVSESTTVSSKVSETDIISEASTSETSASESENSISTVVSE
SEVVEEPAVSLTESESQVSTSEVTSAISETVSTSEEVVLDGLSENINSWNRLSVAPRVSE
TLPSTSETITEAASLFSNYARYSETASSESHSMVAASSEVSIEKLAVSILKDTEGGLYDA
TTIRNIVEMIDSITTNVSYTRSSRQDLVNTASSDNTYSGSQDLNLASKTTTQAGEKGTTE
DLKATIAKTAKSHKWGEHAVAILTAIVLAGAATLAALRNFLMSKKVDK

Enzyme Prediction     

EC	3.2.1.11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH66	156	781	9.7e-165	0.9892086330935251

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam13199	Glyco_hydro_66	3.31e-117	156	779	1	557	Glycosyl hydrolase family 66. This family is a set of glycosyl hydrolase enzymes including cycloisomaltooligosaccharide glucanotransferase (EC:2.4.1.-) and dextranase (EC:3.2.1.11) activities.
cd14745	GH66	7.17e-108	259	633	1	331	Glycoside Hydrolase Family 66. Glycoside Hydrolase Family 66 contains proteins characterized as cycloisomaltooligosaccharide glucanotransferase (CITase) and dextranases from a variety of bacteria. CITase cyclizes part of a (1-6)-alpha-D-glucan (dextrans) chain by formation of a (1-6)-alpha-D-glucosidic bond. Dextranases catalyze the endohydrolysis of (1-6)-alpha-D-glucosidic linkages in dextran. Some members contain Carbohydrate Binding Module 35 (CBM35) domains, either C-terminal or inserted in the domain or both.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AMB81995.1	0.0	1	1248	1	1180
VED87111.1	0.0	1	1248	1	1180
AIY20395.1	0.0	1	1248	1	1180
CCB94514.1	0.0	1	1248	1	1248
QEM33441.1	0.0	1	1248	1	1248

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3VMN_A	4.27e-201	224	782	82	637	Crystalstructure of dextranase from Streptococcus mutans [Streptococcus mutans],3VMO_A Crystal structure of dextranase from Streptococcus mutans in complex with isomaltotriose [Streptococcus mutans],3VMP_A Crystal structure of dextranase from Streptococcus mutans in complex with 4,5-epoxypentyl alpha-D-glucopyranoside [Streptococcus mutans]
5AXG_A	2.59e-44	151	782	44	610	Crystalstructure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus [Thermoanaerobacter pseudethanolicus ATCC 33223],5AXG_B Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus [Thermoanaerobacter pseudethanolicus ATCC 33223]
5AXH_A	2.02e-43	151	782	44	610	Crystalstructure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus, D312G mutant in complex with isomaltohexaose [Thermoanaerobacter pseudethanolicus ATCC 33223],5AXH_B Crystal structure of thermophilic dextranase from Thermoanaerobacter pseudethanolicus, D312G mutant in complex with isomaltohexaose [Thermoanaerobacter pseudethanolicus ATCC 33223]
3WNK_A	1.14e-23	144	780	20	721	ChainA, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans]
3WNP_A	2.54e-23	156	782	13	704	ChainA, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans],3WNP_B Chain B, Cycloisomaltooligosaccharide glucanotransferase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q59979	0.0	184	890	1	707	Dextranase OS=Streptococcus salivarius OX=1304 GN=dex PE=3 SV=1
Q54443	2.49e-198	137	791	93	747	Dextranase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=dexA PE=1 SV=2
P39653	1.10e-171	128	780	142	800	Dextranase OS=Streptococcus downei OX=1317 GN=dex PE=1 SV=1
P94286	9.37e-23	156	781	49	739	Cycloisomaltooligosaccharide glucanotransferase OS=Niallia circulans OX=1397 PE=1 SV=1
P70873	6.51e-20	186	790	70	739	Cycloisomaltooligosaccharide glucanotransferase OS=Niallia circulans OX=1397 GN=cit PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000774	0.997487	0.001152	0.000219	0.000180	0.000153

TMHMM  Annotations     

start	end
1219	1241