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CAZyme Information: MGYG000000119_02205

You are here: Home > Sequence: MGYG000000119_02205

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Blautia sp000432195
Lineage Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia sp000432195
CAZyme ID MGYG000000119_02205
CAZy Family GH36
CAZyme Description Bifunctional alpha-galactosidase/sucrose kinase AgaSK
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
728 MGYG000000119_13|CGC1 83678.41 4.9479
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000119 3510293 Isolate Canada North America
Gene Location Start: 86891;  End: 89077  Strand: -

Full Sequence      Download help

MGIVCKNHVF  TLQTKSSTYQ  MKEEGGFLFH  NYYGPVIGDV  DMSYLVCPMD  RGFSGQPQEI60
VDRRFSLDTR  LLEYSAYGTG  DYRDYCLHAV  YEDGSHVTDL  RFVSYEVKEG  KYALEGLPAM120
YQGEEKAETL  EITLRDEYKN  LEVVLYYGVF  EDLDVITRAC  KIVNQSKEQV  RLLRAYSMGL180
DFDNKDMDML  HFYGRHAMER  KMERSPLQHG  FQGVESRRGY  SSHQQNPFVI  LCKQDANEEY240
GWCYGASFAY  SGNFSIQAEV  SQMDLTRLTV  GIQDTQFEFH  LEPGESFTAP  EVILSFSENG300
LGQLSRNYHK  AIRHNLCRGK  YKTERRPVLI  NNWEATYFDF  TLEKLVAIAK  DAKELGIEML360
VMDDGWFGKR  DSDYSGLGDW  FVNEKKLKGG  LKNLVEEVNK  AGMKFGIWFE  PEMISEDSDL420
YRAHPDWALT  VPGRSFTRAR  HQLVLDFSRE  DVRTYIFDRM  CEILESANIE  YVKWDANRHL480
TDVWSAKLPA  DRQGEVFHRY  ILGLYDFLEK  LTQRFPNLLI  EGCSGGGGRF  DAGMMYYHPQ540
IWCSDDTDAI  ERLDIQYGTS  FGYPISTVGS  HVSVCPNHQT  GRSVSMKTRG  IVAMAGTFGY600
ELDITRLSQE  EKDMVKIQVE  EFKKYYSLIQ  QGDYYRLTDD  GRKSPYVAWE  FAAEDGTEAL660
LNVVTLRVRA  YAMPYTVRIN  GLKPEAVYEV  EGTGEKYSGA  ALINGGYLLP  VIWDDYQSVQ720
VHFVECCE728

Enzyme Prediction      help

EC 3.2.1.22

CAZyme Signature Domains help

Created with Snap36721091451822182542913273644004364735095465826186556919707GH36
Family Start End Evalue family coverage
GH36 9 707 1.1e-267 0.9956395348837209

CDD Domains      download full data without filtering help

Created with Snap36721091451822182542913273644004364735095465826186556917706GalA286632Melibiase325624GH3626282Glyco_hydro_36N646723Glyco_hydro_36C
Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
COG3345 GalA 0.0 7 706 1 664
Alpha-galactosidase [Carbohydrate transport and metabolism].
pfam02065 Melibiase 0.0 286 632 1 347
Melibiase. Glycoside hydrolase families GH27, GH31 and GH36 form the glycoside hydrolase clan GH-D. Glycoside hydrolase family 36 can be split into 11 families, GH36A to GH36K. This family includes enzymes from GH36A-B and GH36D-K and from GH27.
cd14791 GH36 8.34e-144 325 624 1 299
glycosyl hydrolase family 36 (GH36). GH36 enzymes occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-galactosidase, alpha-N-acetylgalactosaminidase, stachyose synthase, and raffinose synthase. All GH36 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH36 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
pfam16875 Glyco_hydro_36N 1.54e-111 26 282 1 256
Glycosyl hydrolase family 36 N-terminal domain. This domain is found at the N-terminus of many family 36 glycoside hydrolases. It has a beta-supersandwich fold.
pfam16874 Glyco_hydro_36C 2.33e-22 646 723 1 78
Glycosyl hydrolase family 36 C-terminal domain. This domain is found at the C-terminus of many family 36 glycoside hydrolases. It has a beta-sandwich structure with a Greek key motif.

CAZyme Hits      help

Created with Snap36721091451822182542913273644004364735095465826186556911726AWY99332.1|GH366723BCN32673.1|GH366720BCK01162.1|GH361723BBF44803.1|GH369722ANU49477.1|GH36
Hit ID E-Value Query Start Query End Hit Start Hit End
AWY99332.1 0.0 1 726 1 726
BCN32673.1 5.70e-306 6 723 8 728
BCK01162.1 6.23e-300 6 720 8 721
BBF44803.1 1.70e-287 1 723 1 725
ANU49477.1 1.05e-284 9 722 11 729

PDB Hits      download full data without filtering help

Created with Snap367210914518221825429132736440043647350954658261865569197252YFN_A107204FNQ_A107204FNR_A107204FNT_A107204FNP_A
Hit ID E-Value Query Start Query End Hit Start Hit End Description
2YFN_A 7.50e-268 9 725 11 719
galactosidasedomain of alpha-galactosidase-sucrose kinase, AgaSK [[Ruminococcus] gnavus E1],2YFO_A GALACTOSIDASE DOMAIN OF ALPHA-GALACTOSIDASE-SUCROSE KINASE, AGASK, in complex with galactose [[Ruminococcus] gnavus E1]
4FNQ_A 5.49e-242 10 720 12 721
Crystalstructure of GH36 alpha-galactosidase AgaB from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNR_A 1.26e-240 10 720 12 721
Crystalstructure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_B Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_C Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNR_D Crystal structure of GH36 alpha-galactosidase AgaA from Geobacillus stearothermophilus [Geobacillus stearothermophilus]
4FNT_A 2.04e-239 10 720 12 721
Crystalstructure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_B Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_C Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus],4FNT_D Crystal structure of GH36 alpha-galactosidase AgaA A355E D548N from Geobacillus stearothermophilus in complex with raffinose [Geobacillus stearothermophilus]
4FNP_A 2.89e-239 10 720 12 721
Crystalstructure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNP_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus [Geobacillus stearothermophilus],4FNS_A Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_B Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_C Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus],4FNS_D Crystal structure of GH36 alpha-galactosidase AgaA A355E from Geobacillus stearothermophilus in complex with 1-deoxygalactonojirimycin [Geobacillus stearothermophilus]

Swiss-Prot Hits      download full data without filtering help

Created with Snap36721091451822182542913273644004364735095465826186556919728sp|G4T4R7|AGASK_RUMGN10720sp|Q9ALJ4|AGAA_GEOSE6723sp|G1UB44|MELA_LACAC7728sp|P43467|AGAL1_PEDPE1725sp|P27756|AGAL_STRMU
Hit ID E-Value Query Start Query End Hit Start Hit End Description
G4T4R7 4.19e-264 9 728 11 722
Bifunctional alpha-galactosidase/sucrose kinase AgaSK OS=Ruminococcus gnavus OX=33038 GN=agaSK PE=1 SV=1
Q9ALJ4 6.90e-240 10 720 12 721
Alpha-galactosidase AgaA OS=Geobacillus stearothermophilus OX=1422 GN=agaA PE=1 SV=1
G1UB44 1.58e-215 6 723 11 728
Alpha-galactosidase Mel36A OS=Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM) OX=272621 GN=melA PE=1 SV=1
P43467 4.63e-215 7 728 10 732
Alpha-galactosidase 1 OS=Pediococcus pentosaceus OX=1255 GN=agaR PE=3 SV=1
P27756 1.45e-179 1 725 1 719
Alpha-galactosidase OS=Streptococcus mutans serotype c (strain ATCC 700610 / UA159) OX=210007 GN=aga PE=3 SV=3

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000042 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000000119_02205.