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CAZyme Information: MGYG000000123_02864

You are here: Home > Sequence: MGYG000000123_02864

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species Blautia sp001304935
Lineage Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia sp001304935
CAZyme ID MGYG000000123_02864
CAZy Family GH38
CAZyme Description hypothetical protein
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
1040 MGYG000000123_8|CGC5 121110.47 4.9474
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000123 5803260 Isolate Canada North America
Gene Location Start: 212132;  End: 215254  Strand: +

Full Sequence      Download help

Enzyme Prediction      help

EC 3.2.1.24

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH38 240 509 2.1e-85 0.9479553903345725

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
COG0383 AMS1 0.0 7 1040 3 943
Alpha-mannosidase [Carbohydrate transport and metabolism].
cd10789 GH38N_AMII_ER_cytosolic 1.24e-125 241 501 2 251
N-terminal catalytic domain of endoplasmic reticulum(ER)/cytosolic class II alpha-mannosidases; glycoside hydrolase family 38 (GH38). The subfamily is represented by Saccharomyces cerevisiae vacuolar alpha-mannosidase Ams1, rat ER/cytosolic alpha-mannosidase Man2C1, and similar proteins. Members in this family share high sequence similarity. None of them have any classical signal sequence or membrane spanning domains, which are typical of sorting or targeting signals. Ams1 functions as a second resident vacuolar hydrolase in S. cerevisiae. It aids in recycling macromolecular components of the cell through hydrolysis of terminal, non-reducing alpha-d-mannose residues. Ams1 utilizes both the cytoplasm to vacuole targeting (Cvt, nutrient-rich conditions) and autophagic (starvation conditions) pathways for biosynthetic delivery to the vacuole. Man2C1is involved in oligosaccharide catabolism in both the ER and cytosol. It can catalyze the cobalt-dependent cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl-enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst.
pfam01074 Glyco_hydro_38 2.96e-91 240 513 1 270
Glycosyl hydrolases family 38 N-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism.
pfam07748 Glyco_hydro_38C 1.66e-76 704 908 1 203
Glycosyl hydrolases family 38 C-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism.
cd10813 GH38N_AMII_Man2C1 2.87e-74 241 500 2 250
N-terminal catalytic domain of mammalian cytosolic alpha-mannosidase Man2C1 and similar proteins; glycoside hydrolase family 38 (GH38). The subfamily corresponds to cytosolic alpha-mannosidase Man2C1 (also known as ER-mannosidase II or neutral/cytosolic mannosidase), mainly found in various vertebrates, and similar proteins. Man2C1 plays an essential role in the catabolism of cytosolic free oligomannosides derived from dolichol intermediates and the degradation of newly synthesized glycoproteins in ER or cytosol. It can catalyze the cleavage of alpha 1,2-, alpha 1,3-, and alpha 1,6-linked mannose residues. Man2C1 is a cobalt-dependent enzyme belonging to alpha-mannosidase class II. It has a neutral pH optimum and is strongly inhitibed by furanose analogs swainsonine (SW) and 1,4-dideoxy-1,4-imino-D-mannitol (DIM), moderately by deoxymannojirimycin (DMM), but not by kifunensine (KIF). DMM and KIF, both pyranose analogs, are normally known to inhibit class I alpha-mannosidase.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
QBE96344.1 0.0 1 1040 1 1040
QIB56865.1 0.0 1 1040 1 1040
QMW80357.1 0.0 1 1040 1 1040
ANU77101.1 0.0 1 1040 1 1040
QQQ94677.1 0.0 1 1040 1 1040

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
6LZ1_A 1.20e-131 56 1040 98 1077
Structureof S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_B Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_C Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-],6LZ1_D Structure of S.pombe alpha-mannosidase Ams1 [Schizosaccharomyces pombe 972h-]
7DD9_A 1.56e-128 56 1040 98 1077
ChainA, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_C Chain C, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_E Chain E, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct],7DD9_G Chain G, Alpha-mannosidase,ZZ-type zinc finger-containing protein P35G2.11c,Maltose/maltodextrin-binding periplasmic protein [synthetic construct]
5JM0_A 2.10e-117 67 996 122 1029
Structureof the S. cerevisiae alpha-mannosidase 1 [Saccharomyces cerevisiae S288C]
1QWN_A 3.68e-09 185 595 11 485
GOLGIALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride [Drosophila melanogaster],1R33_A Golgi alpha-mannosidase II complex with 5-thio-D-mannopyranosylamine [Drosophila melanogaster],1R34_A Golgi alpha-mannosidase II complex with 5-thio-D-mannopyranosylamidinium salt [Drosophila melanogaster],1TQS_A Golgi alpha-Mannosidase II In Complex With Salacinol [Drosophila melanogaster],1TQT_A Golgi alpha-Mannosidase II In Complex With A Diastereomer of Salacinol [Drosophila melanogaster],1TQU_A Golgi alpha-Mannosidase II In Complex With The Salacinol Analog Ghavamiol [Drosophila melanogaster],1TQV_A Golgi alpha-Mannosidase II In Complex With Seleno-Salacinol (Blintol) [Drosophila melanogaster],1TQW_A Golgi alpha-Mannosidase II In Complex With A Diastereomer of Seleno-Salacinol [Drosophila melanogaster],2ALW_A Golgi alpha-mannosidase II complex with Noeuromycin [Drosophila melanogaster],2F18_A GOLGI ALPHA-MANNOSIDASE II complex with (2R,3R,4S)-2-({[(1R)-2-hydroxy-1-phenylethyl]amino}methyl)pyrrolidine-3,4-diol [Drosophila melanogaster],2F1A_A GOLGI ALPHA-MANNOSIDASE II COMPLEX WITH (2R,3R,4S)-2-({[(1S)-2-hydroxy-1-phenylethyl]amino}methyl)pyrrolidine-3,4-diol [Drosophila melanogaster],2F1B_A GOLGI ALPHA-MANNOSIDASE II COMPLEX WITH (2R,3R,4S,5R)-2-({[(1R)-2-hydroxy-1-phenylethyl]amino}methyl)-5-methylpyrrolidine-3,4-diol [Drosophila melanogaster],2F7O_A Golgi alpha-mannosidase II complex with mannostatin A [Drosophila melanogaster],2F7P_A Golgi alpha-mannosidase II complex with benzyl-mannostatin A [Drosophila melanogaster],2F7Q_A Golgi alpha-mannosidase II complex with aminocyclopentitetrol [Drosophila melanogaster],2F7R_A Golgi alpha-mannosidase II complex with benzyl-aminocyclopentitetrol [Drosophila melanogaster],2FYV_A Golgi alpha-mannosidase II complex with an amino-salacinol carboxylate analog [Drosophila melanogaster],2OW6_A Golgi alpha-mannosidase II complex with (1r,5s,6s,7r,8s)-1-thioniabicyclo[4.3.0]nonan-5,7,8-triol chloride [Drosophila melanogaster],2OW7_A Golgi alpha-mannosidase II complex with (1R,6S,7R,8S)-1-thioniabicyclo[4.3.0]nonan-7,8-diol chloride [Drosophila melanogaster],3BLB_A Crystal structure of Golgi Mannosidase II in complex with swainsonine at 1.3 Angstrom resolution [Drosophila melanogaster],3BUB_A Golgi alpha-mannosidase II with an empty active site [Drosophila melanogaster],3D4Y_A GOLGI MANNOSIDASE II complex with mannoimidazole [Drosophila melanogaster],3D4Z_A GOLGI MANNOSIDASE II complex with gluco-imidazole [unidentified],3D50_A GOLGI MANNOSIDASE II complex with N-octyl-6-epi-valienamine [Drosophila melanogaster],3D51_A GOLGI MANNOSIDASE II complex with gluco-hydroxyiminolactam [Drosophila melanogaster],3D52_A GOLGI MANNOSIDASE II complex with an N-aryl carbamate derivative of gluco-hydroxyiminolactam [Drosophila melanogaster],3DDF_A GOLGI MANNOSIDASE II complex with (3R,4R,5R)-3,4-Dihydroxy-5-({[(1R)-2-hydroxy-1 phenylethyl]amino}methyl) pyrrolidin-2-one [Drosophila melanogaster],3DDG_A GOLGI MANNOSIDASE II complex with (3R,4R,5R)-3,4-Dihydroxy-5-({[(1R)-2-hydroxy-1 phenylethyl]amino}methyl) methylpyrrolidin-2-one [Drosophila melanogaster],3DX0_A Golgi alpha-Mannosidase II in complex with Mannostatin A at pH 5.75 [Drosophila melanogaster],3DX1_A Golgi alpha-Mannosidase II in complex with Mannostatin analog (1S,2S,3R,4R)-4-aminocyclopentane-1,2,3-triol [Drosophila melanogaster],3DX2_A Golgi mannosidase II complex with MANNOSTATIN B [Drosophila melanogaster],3DX4_A Golgi alpha-Mannosidase II in complex with Mannostatin analog (1R,2R,3R,4S,5R)-4-amino-5-methoxycyclopentane-1,2,3-triol [Drosophila melanogaster],3EJP_A Golgi alpha-Mannosidase II in complex with 5-substituted swainsonine analog: (5R)-5-[2'-oxo-2'-(phenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJQ_A Golgi alpha-Mannosidase II in complex with 5-substitued swainsonine analog: (5R)-5-[2'-oxo-2'-(4-methylphenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJR_A Golgi alpha-Mannosidase II in complex with 5-substitued swainsonine analog: (5R)-5-[2'-oxo-2'-(4-tert-butylphenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJS_A Golgi alpha-Mannosidase II in complex with 5-substituted swainsonine analog: (5S)-5-[2'-(4-tert-butylphenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJT_A Golgi alpha-Mannosidase II in complex with 5-substituted swainsonine analog:(5R)-5-[2'-(4-tert-butylphenyl)ethyl]-swainsonine [Drosophila melanogaster],3EJU_A Golgi alpha-Mannosidase II in complex with 5-substituted swainsonine analog:(5S)-5-[2'-oxo-2'-(4-tert-butylphenyl)ethyl]-swainsonine [Drosophila melanogaster]
3DX3_A 3.68e-09 185 595 11 485
Golgialpha-Mannosidase II in complex with Mannostatin analog (1R,2R,3S,4R,5R)-5-aminocyclopentane-1,2,3,4-tetraol [Drosophila melanogaster]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
Q54K67 1.89e-142 67 997 83 1039
Alpha-mannosidase G OS=Dictyostelium discoideum OX=44689 GN=manG PE=1 SV=1
Q9NTJ4 2.71e-139 67 1040 81 1035
Alpha-mannosidase 2C1 OS=Homo sapiens OX=9606 GN=MAN2C1 PE=1 SV=1
Q91W89 7.95e-136 67 1040 80 1034
Alpha-mannosidase 2C1 OS=Mus musculus OX=10090 GN=Man2c1 PE=1 SV=1
Q9UT61 2.03e-131 56 1040 98 1077
Alpha-mannosidase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ams1 PE=1 SV=1
P21139 2.50e-130 67 1040 80 1035
Alpha-mannosidase 2C1 OS=Rattus norvegicus OX=10116 GN=Man2c1 PE=1 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000058 0.000000 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000000123_02864.