CAZyme Information: MGYG000000123_03675

Basic Information

• Species: Blautia sp001304935
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia; Blautia sp001304935
• CAZyme ID: MGYG000000123_03675
• CAZy Family: GH30
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2517	MGYG000000123_12|CGC2	275589.92	4.1639

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000123	5803260	Isolate	Canada	North America

• Gene Location: Start: 55751; End: 63304 Strand: -

Full Sequence      

MRSFKRGIAAATTAALCLTSIPLGGLQLVSAADADRVIKLQPGLASTFHDTNEDGLGEFE
GWGTSLCWWANRLGYSDKMTEEAARVFFSDEGLDMNIGRYNVGGGDLVGEPTKVPVNEKA
EFYDLETEGRKPEYKGKKMTVGTNTAMKSLKYTVSDADFGITKGSEVGSFQSIGWINKLD
DEPGDGDNLRYTVKAEETGNYTVKLLLTLTGSNKRDVAIRVNDEKDYLVDADTINSHIIA
SGNNNMLFLVTISDVALNAGDNTINIAGKSDWTLDFVKMAVIKSGEEGKLPEGEEFLHSE
HITRSDSAIPGYATDVTKINESEHELAWYEENYDRADASCGYAWNYNWKADENQINILKA
AAEASGEDFIAEAFSNSPPYFMTNSGCSSGAVDSNKDNLRTDSYRAFAVYMADVIEHWNK
EGVITFQSTTPMNEPYTSYWGAYSNKQEGCHFDQGESQSKIIEMLNEELKKREINIIICG
TDETSIDTAITSYNALSDEAKGIIQRIDTHTYGGSRRAELKELAEKENKNLWMSEVDGAY
TAGTDAGEMSAALGLAKQMMTDVNGLKANAWILWNAIDMHVDKEIKTSSDADYASLEELY
NRVNMNSGYWGIAIGDHDNQEILLTKKYYAYGQLSRYIRPGYTIIGSSDDTLAAYDPESG
KVVVVAVNTAGEDKTWKFDLSSFSTMGDNVTAIRTSGTLKDGENWADVSSEDTIAADTAK
KSITAVLKANSITTYIVDGVTYDSSTEEIVRIDDVNVYTVEGVAAELPDTVEVVTNKNNT
LEKKVTWDLEGVDLTKDTKVTGTVEGTTMTATANVQVVAPNMIYFIDCNSPESPKYAAMD
QYADLLNETADQAYTEGSWGYLDEYGKYNGTVTDEYDTGWYANKDQVIKYTIPLEAGNYK
VNFGFKEWWNQQREMVITAEQDGKTVEIGKTNTWNGNNPWNTYSQDITIENAGDVTFCVA
KQKNNDPVLSFIQIQKVLDLSELQDAITAAGEVDRAQYPAAKLAVLDAAVAAGRELMLKS
ATTQDQIQEGAAAIQDAITGLGEGYTEEQIAANDYVLYLVNCGTSDASVVPDGYRRGLYQ
SNVDQEYSADAETGLSWGYEPNDENSKIVKGGSGAADITSSYIYMADTGVTFIKDVSGFK
YKFEVPQRSNNDYVVTAGFKNPWDTRNVDIKLEGETVESDLKLTKGQLVEKSYKVEVHDG
ELNVMVHSPKRTNQYGDPVLSYLMVEAVAAYTVEGLEAKINSYEASMDGHSYSDATKKDF
DLAVTEARKLIEEQSTDQKAIKNALNALEEAYNALKEVFTYSSITGTNGAQLFDNNGNKI
QAHGGQIQQFTIDGETKYYWYGEDKTNGYRPVVGVHLYTSTDLYNWTDEGAVLKSIPVSE
EDYGKDQEEGYEADLSIFETDEYFSSLYGDYKDQAPDDTENYSSKLEEVYWNLAEDRTVI
ERPKVLYNDATGKYVMWFHADGRTPTNTADYGKARAGVAISDSPTGPFKLLGTYKLHDSA
DANHSWDNVGGAVRDMNLFKDDDGQAYVIYSSDGNLTTYIAKLNADYTGLITDPADAVEG
TGKEGEELTASDYTRNFIDASREAPAMFKYKDKYYIINSGCTGWSPNKAQYAVADHPLGP
WTVMGDPCVGDTKGTTFDTQSTCVFPVDAANGKFIYMGDRWFNPDSGGDLSDSRYVWLPV
EFLPGNQIQLKNYADWTLDELENKASFEVTSELPTVVSSVSEIADVLPGTVMVDFGSGSV
EKEVTWDIGSLDENKLGVAAVTGILTDGNREFTHEISIVNPKLIYFFDSGADTSEYYDTV
NANLLGRLKNAVPDEAYTEENGAGYVGVTNLVDKDKFDLGHHAGDTYLSNGWWAASGKDI
VYAFDLEPGTYTVSAGFQEWWNTGRPSKMTITSGDNLLAEKAFTVYNTSTDLQVNQTFEV
TEETKGKVTVTISKTGNPDPVISWIGVTHNDAAVNKDKLNTLITAADALKKDNYSEETWE
ALSIILEEARIASGNEEADQKAIDEVTKALAKALEDLESTANKDALNNLIAEVDAMDNSI
YTSASWAEYLSAVESARAEAQTVADNKAALKDEIDAAVEALRAAFYAGKEKLVTIQSVLE
KGIAANTVPESSKDSYTEESWADYAAALEAAQALIGSNDEQAVNDAIAALEAAKNALEQK
PEHVVDKTVLTEAIENAPSEEEAGLYTAKSWALYADVLTNAIDVRDSELATQLDVDNAVT
ALTQAQAELVTLEEKLAVMIEGYESHVSSAESYTEESWTIYENALAAAKALQNKPGLTEE
EIDTADLALSEAYNGLTPVEVTVVDKGGLQAVIEQAEQLNKEDYTEEAWKAFEESLNKAK
AVYDNKDASQKDVNKEITALAEAMAELKKHPAKTEEETDKSALKALIDEASQLKKDDYTG
TTWKKFQKAFDDAANIYESETATQEQIDAATAALQAAMDQLEKVTAGPNPKPQPPTDNNK
DKGKNPSRPSGGGSQKGTSSAKTGDETPIGMFAGLGAVALIAIFACTLSILRKRKRS

Enzyme Prediction     

No EC number prediction in MGYG000000123_03675.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH30	300	735	3.6e-123	0.8574561403508771
GH43	1335	1661	1.8e-97	0.9715447154471545

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd18825	GH43_CtGH43-like	8.09e-138	1320	1682	1	285	Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A. This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd08985	GH43_CtGH43-like	3.54e-93	1320	1682	1	273	Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A. This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18821	GH43_Pc3Gal43A-like	2.77e-84	1320	1682	1	262	Glycosyl hydrolase family 43 protein such as Phanerochaete chrysosporium exo-beta-1,3-galactanase (Pc1, 3Gal43A, 1,3Gal43A). This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), Fusarium oxysporum 12S Fo/1 (3Gal), and Streptomyces sp. 19(2012) SGalase1 and SGalase2. It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18822	GH43_CtGH43-like	3.40e-82	1320	1682	1	266	Glycosyl hydrolase family 43 protein such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43). This glycosyl hydrolase family 43 (GH43) subgroup includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43), Streptomyces avermitilis MA-4680 = NBRC 14893 (Sa1,3Gal43A;SAV2109) (1,3Gal43A), and Ruminiclostridium thermocellum ATCC 27405 (Ct1,3Gal43A;CtGH43;Cthe_0661) (1,3Gal43A). It belongs to the GH43_CtGH43 subgroup of the glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) which includes family 43 (GH43) and 62 (GH62) families. GH43_CtGH43 includes proteins such as Clostridium thermocellum exo-beta-1,3-galactanase (Ct1,3Gal43A or CtGH43) which is comprised of the GH43 domain, a CBM13 domain, and a dockerin domain, exhibits an unusual ability to hydrolyze beta-1,3-galactan in the presence of a beta-1,6 linked branch, and is missing an essential acidic residue suggesting a mechanism by which it bypasses beta-1,6 linked branches in the substrate. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.
cd18826	GH43_CtGH43-like	4.77e-75	1320	1682	1	269	Glycosyl hydrolase family 43 protein similar to Clostridium thermocellum exo-beta-1,3-galactanase CtGH43 and Ruminococcus champanellensis arabinanase Ara43A. This uncharacterized glycosyl hydrolase family 43 (GH43) subgroup belongs to a subgroup which includes characterized enzymes with exo-beta-1,3-galactanase (EC 3.2.1.145, also known as galactan 1,3-beta-galactosidase) activity such as Clostridium thermocellum (Ct1,3Gal43A or CtGH43) and Phanerochaete chrysosporium 1,3Gal43A (Pc1, 3Gal43A), and arabinanase (EC 3.2.1.99) activity such as Ruminococcus champanellensis Ara43A. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many GH43 enzymes display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBE99233.1	0.0	1	2516	1	2521
ASU27677.1	0.0	1	2517	1	2523
ANU74869.1	0.0	1	2517	1	2523
QQQ92416.1	0.0	1	2517	1	2523
QJU15030.1	0.0	1	2517	1	2523

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EUG_A	3.16e-61	1314	1699	37	374	TheGH43, Beta 1,3 Galactosidase, BT3683 with galactoimidazole [Bacteroides thetaiotaomicron],6EUH_A The GH43, Beta 1,3 Galactosidase, BT3683 with galactodeoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],6EUH_B The GH43, Beta 1,3 Galactosidase, BT3683 with galactodeoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],6EUH_C The GH43, Beta 1,3 Galactosidase, BT3683 with galactodeoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],6EUI_A The GH43, Beta 1,3 Galactosidase, BT3683 with galactose [Bacteroides thetaiotaomicron VPI-5482]
3VSF_A	1.09e-48	1308	1731	44	381	ChainA, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSF_B Chain B, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSF_C Chain C, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSF_D Chain D, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSF_E Chain E, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSF_F Chain F, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSZ_A Chain A, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSZ_B Chain B, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSZ_C Chain C, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSZ_D Chain D, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSZ_E Chain E, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VSZ_F Chain F, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT0_A Chain A, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT0_B Chain B, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT0_C Chain C, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT0_D Chain D, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT0_E Chain E, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT0_F Chain F, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT1_A Chain A, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT1_B Chain B, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT1_C Chain C, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT1_D Chain D, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT1_E Chain E, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT1_F Chain F, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT2_A Chain A, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT2_B Chain B, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT2_C Chain C, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT2_D Chain D, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT2_E Chain E, Ricin B lectin [Acetivibrio thermocellus ATCC 27405],3VT2_F Chain F, Ricin B lectin [Acetivibrio thermocellus ATCC 27405]
6EUF_A	2.12e-43	1314	1713	13	317	TheGH43, Beta 1,3 Galactosidase, BT0265 [Bacteroides thetaiotaomicron VPI-5482],6EUF_B The GH43, Beta 1,3 Galactosidase, BT0265 [Bacteroides thetaiotaomicron VPI-5482],6EUF_C The GH43, Beta 1,3 Galactosidase, BT0265 [Bacteroides thetaiotaomicron VPI-5482],6EUF_D The GH43, Beta 1,3 Galactosidase, BT0265 [Bacteroides thetaiotaomicron VPI-5482],6EUJ_A The GH43, Beta 1,3 Galactosidase, BT0265 [Bacteroides thetaiotaomicron VPI-5482],6EUJ_B The GH43, Beta 1,3 Galactosidase, BT0265 [Bacteroides thetaiotaomicron VPI-5482],6EUJ_C The GH43, Beta 1,3 Galactosidase, BT0265 [Bacteroides thetaiotaomicron VPI-5482],6EUJ_D The GH43, Beta 1,3 Galactosidase, BT0265 [Bacteroides thetaiotaomicron VPI-5482]
7BYS_A	5.41e-39	1308	1685	5	280	ChainA, Galactan 1,3-beta-galactosidase [Phanerodontia chrysosporium],7BYS_B Chain B, Galactan 1,3-beta-galactosidase [Phanerodontia chrysosporium],7BYT_A Chain A, Galactan 1,3-beta-galactosidase [Phanerodontia chrysosporium]
7BYV_A	1.36e-38	1308	1685	6	281	ChainA, Galactan 1,3-beta-galactosidase [Phanerodontia chrysosporium]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q76FP5	2.30e-45	345	693	125	433	Endo-beta-1,6-galactanase OS=Hypocrea rufa OX=5547 GN=6GAL PE=1 SV=1
A0A401ETL2	2.02e-26	351	741	172	641	Exo-beta-1,6-galactobiohydrolase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=bl1,6Gal PE=1 SV=1
E8MGH9	1.30e-15	2165	2363	1666	1871	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.003605	0.994484	0.000268	0.001021	0.000323	0.000260

TMHMM  Annotations     

start	end
7	29
2489	2511