CAZyme Information: MGYG000000127_05490

Basic Information

• Species: Catenibacillus sp902363555
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Catenibacillus; Catenibacillus sp902363555
• CAZyme ID: MGYG000000127_05490
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase BoGH2A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
821		92097.19	4.8875

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000127	6637359	Isolate	Canada	North America

• Gene Location: Start: 26398; End: 28863 Strand: +

Full Sequence      

MLKQDFNQDWIFTKEDKTAQPCKVTLPHDAMICEARGEQVITGAAGGYFPGGNYTYTKKF
MVPEEDGGRQWLVEFEGAYLDSYVYLNKCFVMSNHSGHRAFTADLSPYLEFGKENVLEVQ
VKNDTVPNVRWYSGSGLYRPVWLYKAGDVHIPINGVRITTPDVERDVSKVVVTVAIENRL
RRTQKIRLAVCLKDADGATASSENYPVTLFPEESPVITVPLYVRDARLWSLDDPYLYTCE
VQLLDDERVMDETVEHFGIRRVQIDPVKGLRLNGERIQLKGSCIHQDHGIIGNADFADAQ
ERKVRLSKAAGFNALRIAHQSASRALLEACDKYGVLLMEESFDQWHQSKVTHDFSRRFDA
QWEQEVEAIVAKDYNHPSVIMYSIGNEILELGRPDGIRMSRRLSEKFRQLDPYRAVTNAV
NGGMTLEGRMFEILMDMGVLSQEQAADMGKLLKGESLDGDEGKMDINDLMTIFAKHMNAI
VAHRFVSENVEEVFSHLDVCGYNYMMARYEGDMANHPNRIILGSETNPPKIDKLWAYTKE
YPACLGDFTWTGWDYIGESGIGITRYNEKKAFGEDYPVYLAYCGDMDITGFRMPVSYYRE
IVFGLRKAPYLSVQAPVHFEDETSNSPWAIPETLESWTWAGNEGKPVKVTVFSASSEVAL
FCNDEEVGRAACGESCGYKAVIETVYCPGELCVVGYTNGKEDGRFSIRTAKPEVQIKSGL
TKTTLSSDGSDLSYLTIEVTDKTGTVHCESERKISLELEGGIKLLGFGSADPYSRENFYD
SCRTTWHGRALAAIRGCAAGPAAVIISAEGCEPVRMEVEIV

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	3	614	1.6e-97	0.5930851063829787

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	5.97e-57	52	418	63	431	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.80e-38	52	417	65	446	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.21e-28	48	416	103	472	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	1.59e-27	48	387	114	462	beta-galactosidase.
pfam18565	Glyco_hydro2_C5	6.21e-24	722	817	7	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CQR57812.1	1.17e-231	1	819	1	806
AEZ64566.1	1.89e-224	1	812	1	808
QFG22228.1	6.23e-224	1	820	1	810
AEM84144.1	7.52e-223	1	818	1	810
ADP81426.1	1.97e-222	1	819	1	819

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6B6L_A	4.47e-142	1	795	5	751	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]
7RSK_A	9.60e-142	1	795	5	751	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7RSK_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
7CWD_A	1.27e-92	1	795	1	775	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	3.07e-91	5	795	11	781	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
5T98_A	7.22e-87	6	791	30	789	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	7.42e-94	3	795	44	822	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	7.93e-67	2	772	47	770	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	3.10e-33	3	414	53	464	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P77989	5.44e-33	5	766	7	695	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KN75	1.68e-28	5	386	30	437	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000085	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000127_05490.