CAZyme Information: MGYG000000134_00074

Basic Information

• Species: Longibaculum muris
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelatoclostridiaceae; Longibaculum; Longibaculum muris
• CAZyme ID: MGYG000000134_00074
• CAZy Family: PL8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1769		199899.97	4.6492

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000134	3162983	Isolate	Canada	North America

• Gene Location: Start: 75318; End: 80627 Strand: -

Full Sequence      

MLKKTNKIILVLTLILSLIFGQGSVVRATSQTDMNLIKSRLKEYFLALDTIDDGAKVETC
YVSQAEDYLKLINEEGAFEDVDYNAHNNAANGAAWSPYLALDRLQAIAIAYHKEGNALYH
SEAAKNGLDKAIKYWSTQGKRDNKPDGPYSTNWWENEVGVPLRFSRIALFMDGIISEEAQ
NILLVKLKEKTPVKYGPGQNNLWFDQNWVYHALLTNDAKELKEMVTDYLDYCLSTQEDDV
TAEAVQVDNSFYMHGRQFYSNGYGMSMFRDMSFWIYMLRGTEFAISQEVVTRMGNYMLNG
TSWTIRGDIMELYLGYRPYKYEVGYKNYAAEYIEPLKRMIDSDPLRANEYQKVLDNIEGK
TTSNGKDGNYYMWRSGYASHMRDGYGVNVKMDSKSVIGGEWRGSWPAGKDGGQLIYWTSS
AASTITVDGDEYTNVFPTYDWAHCPGTTTAARIVKDYANSGRFTNGTDHMIGVTNGKYGA
TSYAMNKKNTQASKGYFFFDDEFVALGAGITSKESVAINTTLNQSEADNVIVNNQTVAEG
TKKKEYTTKWLYNDKVGYVLPKEEKVVVSNALQADNPSLWPEDKKKSTPATFTAWLDHGV
KPVDASYAYIVVPNTTAENVSEYANDIPVTIVSNTKEIQAVRHDGLKQTQINFYKAGTLE
YKPGYTITVDQPCSVMIDESKQQREITVAANDYVAHRTVHVDLSYDEVNTSTVFATRALP
YAGQSITLSEGEDARYVASSYTEGHSPKYVVDENTSTYWESQGNKDEWISVFTGTDQYIK
DMNILWGDQYATEYDVYISKDGENYQLISSVKDGKGKEETINIGKICHYVKIVMKSAKDK
HYQIKELTFNEGKLLSLNQPTTTSSISNVDSSLVGSLAVDGDTSTRWASKRDSNDEWISI
DLGKESNMNAVVINWEAACSDNYQIEVLSDNKNWKTVKSSLKTDQTLTDEINFDEAVTGR
YIKVHSLKSRIVSGKNYGINIFEIKVYGTAANIALNKPAKARSEYSSSLSAKNAFDGSVE
KTNSFQSRWASDRKHNDDWIYVDLQASYEISNIVLNWEDAYAKEYKLQVSEDGENWTDIT
HVTEGKAGITEFNYEEPATGRYVRMLGVEPVGGYGYSIWEFEVYGALVGEQSEPEPEEIN
IALNKPSKASSEFIDENDGKKQYYSSLAFDGSSTTINGKQSRWVSNRDYSNDEWIYVDLE
DTYDISKIVLNWEGTSKHEYKILVSDDQETWQEVVHRTDGVGVQEIKLDELITGRYVKMQ
GVKVGAKYGYSLWEFEVYGELTEKPNTTNIALNKPSKASSEFIDPNDGNKQYYSSLAFDG
NTDKINDQQSRWVSNRKSNDEWIYVDLQDNYYISKIVLNWEGACGKEYKLQVSNDGENWT
DITHVTDGKKGITEFNYGEPATGRYVRMLGIQPVGEYGYSLWEFEVYGVSLKGDLKAYYD
ENKNIDTSLYTSASVSKYHEALENVIAVYKNKKATPEEILNAKKQLKNAIDSLVKKADKK
ALEEVINKAQEVDKELYIQDTVKELEKVLEDVKVIYEDENATQKQVDKAVESLNAAIKAL
VKKADKKALEEAINKAQEIDKELYIQDTVKELEKVLEDVKAIYEDEKATQKQVDKAVEDL
NAAIKALVKKADKKALEKAIQEAKVIDTKLYTDETVEVLTAVLKEAEEIYKDETVTQETV
DAIVKKLQEAVEKLEEKEVDIVVTPDKPDENKPDENKPGEPEQNNNQPTTEEKVETSDNT
QYQMYIGLLGISMCAMALLINKKKKNQVK

Enzyme Prediction     

No EC number prediction in MGYG000000134_00074.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL8	372	614	1.3e-54	0.9799196787148594
CBM32	1001	1122	5.3e-26	0.9193548387096774
CBM32	865	984	3.3e-24	0.9112903225806451
CBM32	1311	1425	3.5e-24	0.8629032258064516
CBM32	1160	1276	1.3e-21	0.8790322580645161

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01083	GAG_Lyase	2.38e-95	95	689	61	691	Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
pfam02278	Lyase_8	9.79e-55	369	616	1	252	Polysaccharide lyase family 8, super-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.
pfam00754	F5_F8_type_C	6.56e-21	864	969	3	115	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	3.12e-19	998	1120	1	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	2.78e-17	1297	1423	3	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQY28197.1	0.0	1	1554	1	1698
QMW73769.1	0.0	1	1554	1	1698
QPS12968.1	0.0	1	1554	1	1698
QQV04833.1	0.0	1	1554	1	1698
QUN12787.1	0.0	1	1554	1	1718

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CB8_A	6.66e-58	65	689	28	637	CHONDROITINASEAC LYASE FROM FLAVOBACTERIUM HEPARINUM [Pedobacter heparinus]
1HM2_A	9.61e-58	65	689	50	659	ACTIVESITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HM3_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-Oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus],1HMU_A ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HMW_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus]
2E22_A	6.79e-27	47	704	20	710	Crystalstructure of xanthan lyase in complex with mannose [Bacillus sp. GL1]
2E24_A	1.56e-26	47	704	20	710	ChainA, Xanthan lyase [Bacillus sp. GL1]
1J0M_A	2.71e-26	47	704	20	710	CrystalStructure of Bacillus sp. GL1 Xanthan Lyase that Acts on Side Chains of Xanthan [Bacillus sp. GL1],1J0N_A Crystal Structure of Bacillus sp. GL1 Xanthan Lyase that Acts on Side Chains of Xanthan [Bacillus sp. GL1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q59288	5.26e-57	65	689	50	659	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=cslA PE=1 SV=1
Q9AQS0	1.39e-26	9	778	5	820	Xanthan lyase OS=Bacillus sp. (strain GL1) OX=84635 GN=xly PE=1 SV=1
P59807	2.56e-07	213	692	458	961	Chondroitin sulfate ABC endolyase OS=Proteus vulgaris OX=585 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000297	0.999022	0.000208	0.000155	0.000152	0.000150

TMHMM  Annotations     

start	end
7	26
1742	1761