CAZyme Information: MGYG000000134_00625

Basic Information

• Species: Longibaculum muris
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelatoclostridiaceae; Longibaculum; Longibaculum muris
• CAZyme ID: MGYG000000134_00625
• CAZy Family: GH3
• CAZyme Description: Beta-hexosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2143		236477.64	4.4509

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000134	3162983	Isolate	Canada	North America

• Gene Location: Start: 95257; End: 101688 Strand: -

Full Sequence      

MLYGVDAVHGNNNVEDATVYPHNIGLGQANDAELMKKIGKATAQEVRAIGANWAFTPTLG
LPQNERWGRTYECFSETSEISSKLGAALIEGMQGDLSSQYAIATAKHYIGEGIAKNGINQ
GDIDGKLFEKELEELLIPYRKAIASGVKSVMVTYSSIDGVKCHENKNLITDILKGDLGFK
GIVITDYNGTDQVSGSTYKEKIEKTINAGMDMLMIDGNEGNTPKWLLARDNIMKSVEEKK
ISLERIDDAVRRILTVKCELGLLDDVKLAYADQELLAQFGSDEHRQIAREAVRKSLTLLK
NSETQNGSTLMADLKNMKKIIVAGETGDDIGLQCGGWTISWQGSKGKITKGTTIYSGLRE
LAGKDKTIDYNANGYFEEDDYEAAIVVVGERPYAESNGDRSARDLTIDADDIKAINRIKQ
QHPDLPIIAVLVTGRPITIVDQVDDLDAIVMAGFPGTEGAGVADVLLGDYDFTGHLTMTW
PWYAQDIEKKLTDETKVMFEYGRGLTKNQTTAIANPKPNDPSVIDLSKTGGIVQAEDFYS
KHSKIVLENNNTSIGYFWNGYDISYNVIVPKKARYTLNVAAATQNNGVKAAFDVYVDNVL
YYSTSTPALNTGGWASFEEQEMNDKISLPDGKHIIKFVSQTKDLNIDYFKFIEFDDEYQE
PEIKPDVPNEGSGAMIKEDAVKVTMSSSDNSGSMTWYAGNQEITNRNTEKAALDIRNADD
SKMTTITVDEDKTYQSVLGMGTSVEESTVNNLMKMDDASRRKFIKRLVDPVNGMGNTLFR
LTIGTADFTGRPFYTYYDGTGKELDGQPDWENKTGHGFSIEKDKQYGIISVIKEIQEIAK
ECGVEKDLKFFASSWTPPGWMKVATSSSQSCENNELLLKGGKLNDVYIDELGKYYVRFIE
EYQKAGIPIYAMTLQNEPLLEIDYPSCYITGGQEAKLAKAIKQELAKSKVLNDQEKDVKV
WAFDHNFDGAQAYVADLFATADGRENVDGIAFHPYGGSPSTMGSLYDQYKEDYTMHLTER
SVWGASGVSDIISWFRNGSESYNAWVTMLDSKIAPHQWVGTPDPTLFVQDAYNRNKYWAT
PEVYLIGQLTKYVRPGFVRIASSSGSTSSVTNVAFKDPETGKIVMVVANSTGSKQEFKVV
NNGTQFNATLPAGNVATYIWDPVDGESYKNITDDLTLDDALLEGKGEIENGELGHIDSTT
KLNYTVNVKQPGTYKVELDVAVGGEEGKNFPVVISQNDKVLGTAHTRRYWLWGGEWNRYS
KIQTYVTLEEAGLQNISITFPEAGMNFKKVQFTLADAVQSIPGKLDTTNYFDKNGMVLEA
GKIPNFGFSQLDYYLDYKINVQESGNYNFDFEIATKDDKAGAWIDLLNPDGSKNKYLGEI
NFENTKDTDTYAHNTKAFDLEAGEYTIRIKFKSGDTNFRNVVIGQAISLDYEELKEGALN
NKELIVKLSGASFNANLTKENWSLDLPEGLNFKLERVNDSTVKIILTGEATQDFDTDLKV
LLNVDAKEFNGLEGTSLVQYVNIKAVDDEEVLVALDEVEFNSETLNLTIKGGTFKDDVAK
HLTLTGDAAQYVSIKEVKQISHTELQVQLVWKPMYDHYKGTISITSDGYSDGTLSLEKEI
TFKATDQLPTAIEVGPINSVSLAENLAYRQKGSLAKNPAKGNYVDFFLDVKEAGQYTISY
SVANSEAVVNGLKLSGGALLATDNIGSVSFNKFWGNALGYVQTLTLKAGQQTLRFEMNNY
SDGFKIENIKIAATKAPTEINKKTTISVDQLYDGSKTKGWGIEVKDKISNIGCSEAGSYQ
DYLVDVKEDGYYNFSIKAARSSSTSVNAILQQVTDKVTDLGIVSIANTGDWGKFVDSEST
MVKLLAGQQILRVYDDGDGFNYRSFTLDYVKDIDKEAPVITGKDVIVYAGSDISATDAIA
LAIEDNVDGTIDINSELVNIETTFDSHKVGEYKVVVTAYDVEGNKSEKTFTITVVSPATI
EYSNDPMKVGDEFDPFENVRILDVDGKDITSKAEVISNNVNVDEVGEYEVVYQVVDKYGT
KTTLTRIVKVEKADTPDTPDNPDTPDNPDTPDNPDTPDKPDINVKPDEPTNPDTPSVDVT
PGTPDQSVVVTEDTTNMNYYLVAGCIALLAIVVVSKKRKSRKA

Enzyme Prediction     

No EC number prediction in MGYG000000134_00625.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH30	721	1160	3.3e-107	0.9952038369304557
GH3	1	214	2.6e-63	0.9444444444444444

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	1.61e-58	1	481	106	600	beta-glucosidase BglX.
pfam00933	Glyco_hydro_3	3.62e-53	1	256	68	316	Glycosyl hydrolase family 3 N terminal domain.
COG5520	XynC	1.98e-32	726	1161	36	429	O-Glycosyl hydrolase [Cell wall/membrane/envelope biogenesis].
pfam01915	Glyco_hydro_3_C	1.92e-29	296	506	1	215	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
PLN03080	PLN03080	2.88e-23	14	480	108	588	Probable beta-xylosidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQV06088.1	0.0	1	2057	104	2169
QPS14204.1	0.0	1	2057	104	2169
QMW75461.1	0.0	1	2057	104	2169
QQY26973.1	0.0	1	2057	104	2169
BCL58477.1	0.0	1	2055	105	2177

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5M6G_A	8.57e-120	1	506	124	613	Crystalstructure Glucan 1,4-beta-glucosidase from Saccharopolyspora erythraea [Saccharopolyspora erythraea D]
6JGC_A	4.45e-105	1	510	94	606	Crystalstructure of barley exohydrolaseI W286Y mutant in complex with glucose. [Hordeum vulgare subsp. vulgare],6JGD_A Crystal structure of barley exohydrolaseI W286Y mutant in complex with methyl 6-thio-beta-gentiobioside [Hordeum vulgare subsp. vulgare]
6JG7_A	1.51e-104	1	510	94	606	Crystalstructure of barley exohydrolaseI W286F in complex with methyl 2-thio-beta-sophoroside [Hordeum vulgare subsp. vulgare],6JGA_A Crystal structure of barley exohydrolaseI W286F in complex with 4'-nitrophenyl thiolaminaribioside [Hordeum vulgare subsp. vulgare],6JGB_A Crystal structure of barley exohydrolaseI W286F mutant in complex with methyl 6-thio-beta-gentiobioside [Hordeum vulgare subsp. vulgare]
1LQ2_A	1.69e-104	1	510	90	602	Crystalstructure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with gluco-phenylimidazole [Hordeum vulgare],1X38_A crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with gluco-phenylimidazole [Hordeum vulgare],1X39_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with gluco-phenylimidazole [Hordeum vulgare]
1EX1_A	1.83e-104	1	510	90	602	Beta-d-glucanExohydrolase From Barley [Hordeum vulgare],1IEQ_A Crystal Structure Of Barley Beta-D-Glucan Glucohydrolase Isoenzyme Exo1 [Hordeum vulgare],1IEV_A Crystal Structure Of Barley Beta-d-glucan Glucohydrolase Isoenzyme Exo1 In Complex With Cyclohexitol [Hordeum vulgare],1IEW_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 2-deoxy-2-fluoro-alpha-D-glucoside [Hordeum vulgare],1IEX_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 4I,4III,4V-S-trithiocellohexaose [Hordeum vulgare],1J8V_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme Exo1 in complex with 4'-nitrophenyl 3I-thiolaminaritrioside [Hordeum vulgare],3WLH_A Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare],3WLJ_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 3-deoxy-glucose [Hordeum vulgare subsp. vulgare],3WLK_X Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with 4-deoxy-glucose [Hordeum vulgare subsp. vulgare],3WLL_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with PEG400 [Hordeum vulgare subsp. vulgare],3WLM_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme exo1 in complex with octyl-O-glucoside [Hordeum vulgare subsp. vulgare],3WLN_A Crystal structure of barley beta-D-glucan glucohydrolase isoenzyme EXO1 in complex with octyl-S-glucoside [Hordeum vulgare subsp. vulgare],3WLP_A Crystal Structure Analysis of Plant Exohydrolase [Hordeum vulgare subsp. vulgare]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q23892	1.61e-57	1	518	171	714	Lysosomal beta glucosidase OS=Dictyostelium discoideum OX=44689 GN=gluA PE=1 SV=2
A7LXU3	3.36e-56	2	481	127	617	Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02659 PE=1 SV=1
T2KMH0	1.04e-48	1	481	68	563	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
P33363	5.45e-43	1	481	106	600	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
Q56078	7.26e-43	1	481	106	600	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
2117	2134