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CAZyme Information: MGYG000000137_01818

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Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species UBA7160 sp902363665
Lineage Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; UBA7160; UBA7160 sp902363665
CAZyme ID MGYG000000137_01818
CAZy Family GT4
CAZyme Description Glycosyltransferase Gtf1
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
978 MGYG000000137_7|CGC4 109314.01 6.7621
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000000137 4141348 Isolate United Kingdom Europe
Gene Location Start: 205937;  End: 208873  Strand: +

Full Sequence      Download help

Enzyme Prediction      help

No EC number prediction in MGYG000000137_01818.

CAZyme Signature Domains help

Family Start End Evalue family coverage
GT4 808 950 5.5e-28 0.90625

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
cd03808 GT4_CapM-like 1.44e-116 621 974 1 358
capsular polysaccharide biosynthesis glycosyltransferase CapM and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. CapM in Staphylococcus aureus is required for the synthesis of type 1 capsular polysaccharides.
pfam02397 Bac_transf 1.36e-70 91 267 1 178
Bacterial sugar transferase. This Pfam family represents a conserved region from a number of different bacterial sugar transferases, involved in diverse biosynthesis pathways.
COG2148 WcaJ 1.77e-66 90 265 40 216
Sugar transferase involved in LPS biosynthesis (colanic, teichoic acid) [Cell wall/membrane/envelope biogenesis].
cd05232 UDP_G4E_4_SDR_e 1.40e-58 318 613 1 303
UDP-glucose 4 epimerase, subgroup 4, extended (e) SDRs. UDP-glucose 4 epimerase (aka UDP-galactose-4-epimerase), is a homodimeric extended SDR. It catalyzes the NAD-dependent conversion of UDP-galactose to UDP-glucose, the final step in Leloir galactose synthesis. This subgroup is comprised of bacterial proteins, and includes the Staphylococcus aureus capsular polysaccharide Cap5N, which may have a role in the synthesis of UDP-N-acetyl-d-fucosamine. This subgroup has the characteristic active site tetrad and NAD-binding motif of the extended SDRs. Extended SDRs are distinct from classical SDRs. In addition to the Rossmann fold (alpha/beta folding pattern with a central beta-sheet) core region typical of all SDRs, extended SDRs have a less conserved C-terminal extension of approximately 100 amino acids. Extended SDRs are a diverse collection of proteins, and include isomerases, epimerases, oxidoreductases, and lyases; they typically have a TGXXGXXG cofactor binding motif. SDRs are a functionally diverse family of oxidoreductases that have a single domain with a structurally conserved Rossmann fold, an NAD(P)(H)-binding region, and a structurally diverse C-terminal region. Sequence identity between different SDR enzymes is typically in the 15-30% range; they catalyze a wide range of activities including the metabolism of steroids, cofactors, carbohydrates, lipids, aromatic compounds, and amino acids, and act in redox sensing. Classical SDRs have an TGXXX[AG]XG cofactor binding motif and a YXXXK active site motif, with the Tyr residue of the active site motif serving as a critical catalytic residue (Tyr-151, human 15-hydroxyprostaglandin dehydrogenase numbering). In addition to the Tyr and Lys, there is often an upstream Ser and/or an Asn, contributing to the active site; while substrate binding is in the C-terminal region, which determines specificity. The standard reaction mechanism is a 4-pro-S hydride transfer and proton relay involving the conserved Tyr and Lys, a water molecule stabilized by Asn, and nicotinamide. Atypical SDRs generally lack the catalytic residues characteristic of the SDRs, and their glycine-rich NAD(P)-binding motif is often different from the forms normally seen in classical or extended SDRs. Complex (multidomain) SDRs such as ketoreductase domains of fatty acid synthase have a GGXGXXG NAD(P)-binding motif and an altered active site motif (YXXXN). Fungal type ketoacyl reductases have a TGXXXGX(1-2)G NAD(P)-binding motif.
TIGR03025 EPS_sugtrans 4.47e-53 90 259 257 429
exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase. Members of this family are generally found near other genes involved in the biosynthesis of a variety of exopolysaccharides. These proteins consist of two fused domains, an N-terminal hydrophobic domain of generally low conservation and a highly conserved C-terminal sugar transferase domain (pfam02397). Characterized and partially characterized members of this subfamily include Salmonella WbaP (originally RfbP), E. coli WcaJ, Methylobacillus EpsB, Xanthomonas GumD, Vibrio CpsA, Erwinia AmsG, Group B Streptococcus CpsE (originally CpsD), and Streptococcus suis Cps2E. Each of these is believed to act in transferring the sugar from, for instance, UDP-glucose or UDP-galactose, to a lipid carrier such as undecaprenyl phosphate as the first (priming) step in the synthesis of an oligosaccharide "block". This function is encoded in the C-terminal domain. The liposaccharide is believed to be subsequently transferred through a "flippase" function from the cytoplasmic to the periplasmic face of the inner membrane by the N-terminal domain. Certain closely related transferase enzymes, such as Sinorhizobium ExoY and Lactococcus EpsD, lack the N-terminal domain and are not found by this model.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
QUO31262.1 0.0 1 925 1 960
QMW80818.1 1.79e-180 90 975 34 1022
QIB56408.1 1.79e-180 90 975 34 1022
QQY28407.1 2.35e-170 620 977 2 359
QQV06535.1 2.32e-167 620 977 2 359

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
5W7L_A 7.40e-30 84 289 4 205
Structureof Campylobacter concisus PglC I57M/Q175M variant [Campylobacter concisus 13826],5W7L_B Structure of Campylobacter concisus PglC I57M/Q175M variant [Campylobacter concisus 13826]
7EC2_A 2.14e-12 642 943 151 458
ChainA, Glycosyl transferase, group 1 family protein [Staphylococcus aureus subsp. aureus USA300],7EC2_B Chain B, Glycosyl transferase, group 1 family protein [Staphylococcus aureus subsp. aureus USA300]
1HZJ_A 3.93e-08 323 555 41 292
HumanUdp-Galactose 4-Epimerase: Accommodation Of Udp-N- Acetylglucosamine Within The Active Site [Homo sapiens],1HZJ_B Human Udp-Galactose 4-Epimerase: Accommodation Of Udp-N- Acetylglucosamine Within The Active Site [Homo sapiens]
1I3K_A 9.20e-08 323 555 41 292
ChainA, UDP-GLUCOSE 4-EPIMERASE [Homo sapiens],1I3K_B Chain B, UDP-GLUCOSE 4-EPIMERASE [Homo sapiens],1I3L_A Chain A, UDP-GLUCOSE 4-EPIMERASE [Homo sapiens],1I3L_B Chain B, UDP-GLUCOSE 4-EPIMERASE [Homo sapiens],1I3M_A Chain A, UDP-GLUCOSE 4-EPIMERASE [Homo sapiens],1I3M_B Chain B, UDP-GLUCOSE 4-EPIMERASE [Homo sapiens],1I3N_A Chain A, UDP-GLUCOSE 4-EPIMERASE [Homo sapiens],1I3N_B Chain B, UDP-GLUCOSE 4-EPIMERASE [Homo sapiens]
1EK5_A 1.22e-07 323 555 41 292
StructureOf Human Udp-galactose 4-epimerase In Complex With Nad+ [Homo sapiens],1EK6_A Structure Of Human Udp-galactose 4-epimerase Complexed With Nadh And Udp-glucose [Homo sapiens],1EK6_B Structure Of Human Udp-galactose 4-epimerase Complexed With Nadh And Udp-glucose [Homo sapiens]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
Q0P9C9 2.49e-30 621 975 2 367
N,N'-diacetylbacillosaminyl-diphospho-undecaprenol alpha-1,3-N-acetylgalactosaminyltransferase OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) OX=192222 GN=pglA PE=1 SV=1
Q0P9D0 8.91e-29 86 277 2 189
Undecaprenyl phosphate N,N'-diacetylbacillosamine 1-phosphate transferase OS=Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) OX=192222 GN=pglC PE=1 SV=1
P71241 1.08e-28 88 259 271 448
UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase OS=Escherichia coli (strain K12) OX=83333 GN=wcaJ PE=1 SV=2
P10498 6.67e-27 90 265 5 190
Exopolysaccharide production protein PSS OS=Rhizobium leguminosarum bv. phaseoli OX=385 GN=pss PE=3 SV=1
Q44576 7.63e-26 91 259 338 516
Undecaprenyl-phosphate glucose phosphotransferase OS=Komagataeibacter xylinus OX=28448 GN=aceA PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

0.980814 0.018403 0.000479 0.000111 0.000060 0.000151

TMHMM  Annotations      download full data without filtering help

start end
11 30
96 118