CAZyme Information: MGYG000000138_04141

Basic Information

• Species: Parabacteroides johnsonii
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; Parabacteroides johnsonii
• CAZyme ID: MGYG000000138_04141
• CAZy Family: GH3
• CAZyme Description: Beta-hexosaminidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
994		110818.28	8.8926

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000138	4917599	Isolate	United Kingdom	Europe

• Gene Location: Start: 12945; End: 15929 Strand: -

Full Sequence      

MKKIYVFLFSCVAVLSAVAQTTPNLYRAVDKVKMNHWVDSVFDAMSYDERVGQLFMVIAN
PKSDTRNMQRLMRYVNEIKIGGILFHKGDPVMQAEVTNRLQKTSRVPMLVSLDGEWGLSM
RLSGTTRFPKNMMLGAIEDNDLITEYGKEVARQCKEMGIHINFAPDMDVNSNTDNPVIGL
RSFGENPQAVADKGLAYARGLEGQGILSVAKHFPGHGDTAEDSHYTLPAVHHDRARLDSV
ELLPFKRYIYDGYAGVMTGHLYVPALDKVRNRPVSMSRAVVTDLLQKELGFRGLCFTDAL
AMKGATTRKSDNPSVKALLAGNDILLAPAAPINDFAAVKEALEEGILDREEIEAKIIKIL
QYKYIAGLDDYRPIETKGLSERLNSPHAAWLAAKLNEEAITLLKNEGDIIPLKQLNKKRI
AALSIGDGVGNEFQKMLGRYDSIACFSISRNSTAAQVQNVYKKLEKYDVIICGVHTVRIP
ESLALHQLAAKKELIYAFFTLPYFCKEYKSSILKAKAVIMAYEGTPLAQKYAAQAIFGGI
AVKGKLPVSIPTLYTAGTGMFTEKTRLGYHEPEEVGASPERLDVIASIVEDGLEQKAFPG
CQVLVAKDGMIIYDKSFGYFDYDKKQAVDENSIYDLASSSKAAGTLLAVMKAYDDKKFTL
NNKISDFIPELKDSDKKNLVIKDLLYHQSGLTPTINFYLNAIDRDSYKGSLYSNVKNQAH
PVRFDARTYVRNDFSFLSGLVSFDRRPGFTTEVAHKMYLHDSFKDTIIQEIKDSRLGVRG
KYKYSCINFILLKMMVEKQMQQPMDRLLHDTFFSRLGAWHTTYNPLHIFDTIQIVPTEYD
GFIRRQLLRGYVHDEAAAFQGGVSGNAGLFSNAGDLAKVLQLYLNQGHYGGEGYLSKETC
RLFTQSKSPTCRRGLGFDKPVAGGGKASPCGSLAPISVYGHTGFTGTCFWVDPDNNLLYI
FLSNRVNPTRANNKLSSLNIRTRIQDAIYKAINN

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	101	326	2.3e-56	0.9768518518518519

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1472	BglX	4.07e-76	45	431	1	368	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam00933	Glyco_hydro_3	6.87e-71	48	360	3	314	Glycosyl hydrolase family 3 N terminal domain.
PRK03642	PRK03642	2.60e-58	570	992	28	431	putative periplasmic esterase; Provisional
pfam00144	Beta-lactamase	2.27e-41	585	970	1	313	Beta-lactamase. This family appears to be distantly related to pfam00905 and PF00768 D-alanyl-D-alanine carboxypeptidase.
PRK05337	PRK05337	1.57e-34	80	394	26	326	beta-hexosaminidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT50763.1	0.0	1	992	1	992
QUT21701.1	0.0	1	992	1	991
QCY58561.1	0.0	1	992	1	991
QIX64335.1	0.0	1	992	1	991
BBK92319.1	0.0	1	992	1	991

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6K5J_A	5.81e-64	44	550	10	531	Structureof a glycoside hydrolase family 3 beta-N-acetylglucosaminidase from Paenibacillus sp. str. FPU-7 [Paenibacillaceae]
3BMX_A	8.62e-62	27	569	31	632	Beta-N-hexosaminidase(YbbD) from Bacillus subtilis [Bacillus subtilis],3BMX_B Beta-N-hexosaminidase (YbbD) from Bacillus subtilis [Bacillus subtilis],3NVD_A Structure of YBBD in complex with pugnac [Bacillus subtilis],3NVD_B Structure of YBBD in complex with pugnac [Bacillus subtilis]
3LK6_A	2.49e-61	27	569	5	606	ChainA, Lipoprotein ybbD [Bacillus subtilis],3LK6_B Chain B, Lipoprotein ybbD [Bacillus subtilis],3LK6_C Chain C, Lipoprotein ybbD [Bacillus subtilis],3LK6_D Chain D, Lipoprotein ybbD [Bacillus subtilis]
4GYJ_A	4.51e-61	27	569	35	636	Crystalstructure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYJ_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1) [Bacillus subtilis subsp. subtilis str. 168],4GYK_A Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168],4GYK_B Crystal structure of mutant (D318N) bacillus subtilis family 3 glycoside hydrolase (nagz) in complex with glcnac-murnac (space group P1211) [Bacillus subtilis subsp. subtilis str. 168]
3SQL_A	5.42e-54	71	363	50	346	CrystalStructure of Glycoside Hydrolase from Synechococcus [Synechococcus sp. PCC 7002],3SQL_B Crystal Structure of Glycoside Hydrolase from Synechococcus [Synechococcus sp. PCC 7002],3SQM_A Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002],3SQM_B Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002],3SQM_C Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002],3SQM_D Crystal Structure of Glycoside Hydrolase from Synechococcus Complexed with N-acetyl-D-glucosamine [Synechococcus sp. PCC 7002]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P40406	4.72e-61	27	569	31	632	Beta-hexosaminidase OS=Bacillus subtilis (strain 168) OX=224308 GN=nagZ PE=1 SV=1
P48823	4.51e-49	71	413	55	426	Beta-hexosaminidase A OS=Pseudoalteromonas piscicida OX=43662 GN=cht60 PE=1 SV=1
P77619	5.40e-42	572	965	30	395	Putative D-alanyl-D-alanine carboxypeptidase OS=Escherichia coli (strain K12) OX=83333 GN=yfeW PE=1 SV=2
Q8XBJ0	1.50e-40	572	965	30	395	Putative D-alanyl-D-alanine carboxypeptidase OS=Escherichia coli O157:H7 OX=83334 GN=yfeW PE=3 SV=1
Q7WUL3	3.03e-40	37	428	18	414	Beta-N-acetylglucosaminidase/beta-glucosidase OS=Cellulomonas fimi OX=1708 GN=nag3 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000886	0.993607	0.004776	0.000234	0.000230	0.000235

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000138_04141.