CAZyme Information: MGYG000000139_00209

Basic Information

• Species: Eubacterium_G sp000434315
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eubacterium_G; Eubacterium_G sp000434315
• CAZyme ID: MGYG000000139_00209
• CAZy Family: GH87
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1327		144983.45	4.5372

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000139	2758071	Isolate	United Kingdom	Europe

• Gene Location: Start: 245569; End: 249552 Strand: +

Full Sequence      

MRKSIQKVLSFALAGAMVLQPLPSAQSVFKVNAGVASESKFDIPQADASGRVGAKVPYTR
YDSLQANIGGGAQIASSKNFDRMNIASQASEQSYVRLPGKGAYTEWTMNTTGDGVTMRFT
MPDSSDGMGLKGSLDVYVNGQYKQTIDLTSYFMWQYFASGNPSDNNDGGVPCFAFDEVHF
KLDNSLKKGDTIRIQSSGANALEYGVDFLEIENVPDEIAQPDNSLNVEDFGAVPDDGQDD
YDAIYRCIEEADRSNMDVYIPAGTFEIGQVWRLYGSNMKITGAGMWYTNIQFTNPDAGGG
GISAGWQTSGSKDGYCNNIEFCNMYINSNLRSRYNQQAVYKCFMDVFTDGSVIHDIWEEH
FECGFWFGDYNGKMDYSDGVKVMDCRIRNNLADGVNFCQGTSNATVYNCSIRNNGDDGLA
CWNNSWGGAKNESGNVFAYNTIDFIWRAGGIAIYGGDNFKVYNNYICDTFMAAGIHLNTT
FDGYKFSECKNMTFDNNIIVRAGCTKDSWGEELGAVDIKQEVKNVTFNNTQIYDAQHDGI
RILDNSASNVVFNNTKILGSGIDGQQGNYSSVPHAGAAIRLGSSSSATFNGIEIANSAFK
NKEDGSPYFYTGGTDSSCVKNATIYDDDVTYTVPGYPEPYIDGGQQGGVTNPLEGITGYD
IALTGLTWKNENGSSDLKNGDKVTFSVAITNTSNVDIPEGVTIGFKVVVDGTTTYRNQKF
KDGLKAGQTVKLSTTGAWTASRGGHTIVATADDTNKLPNETDKNNNTRTKKFNVYGDAAT
NPVTRVSGGYDLVVSKIVANKQDIAVGDQLVFTATITNAGDTAIPAGTTIGYQLQIDGNT
SNILWCDKYNSGLAAGQSVDLTVNSGSAQRNYWTATSGKHSIMAWVDDVNRIQNEVNEDN
NQYTISLDIPTASMIENPDAPDDLNNPGQVETTKPVETTTKAPETTKPVETTTKAPETAK
PVETTTKTPETSRDTSEDIVINEFMKTWTVGTGNGYQYMGQVNYNAPAGYRYLQLTYTGD
TRAFEELRLEFFTDMKNPYWFNKNQAVKFVTTSGDVVEAPSSTAKTVVIDLQKSGLNINQ
TNTGFHIHNTQGYGSFTITDARLYKTAAVEQPTTKPVETTTKAPETTKAPETTKPVETTT
EAPSMVSDKVIVHGYQMSAKFNGVEGDMGIRIVYSVEPEIDGKKVEKAGVICGIVYGDNP
ITKNDIVLNSKNSYVASYEATDKGLIGDVVGDSKTAKNYVRTFGLKDYNAEGLSAKYYTR
SYAILEDGTIAYSNVEEFNIYEIGKKIYQKRSMVNEAAHNYLYDKILKVVNPAELPIDYN
WSNTIEK

Enzyme Prediction     

EC	3.2.1.59

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH87	52	603	6.1e-162	0.8408710217755444

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14490	CBM6-CBM35-CBM36_like_1	1.25e-44	53	211	1	156	uncharacterized members of the carbohydrate binding module 6 (CBM6) and CBM35_like superfamily. Carbohydrate binding module family 6 (CBM6, family 6 CBM), also known as cellulose binding domain family VI (CBD VI), and related CBMs (CBM35 and CBM36). These are non-catalytic carbohydrate binding domains found in a range of enzymes that display activities against a diverse range of carbohydrate targets, including mannan, xylan, beta-glucans, cellulose, agarose, and arabinans. These domains facilitate the strong binding of the appended catalytic modules to their dedicated, insoluble substrates. Many of these CBMs are associated with glycoside hydrolase (GH) domains. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold. CBM36s are calcium-dependent xylan binding domains. CBM35s display conserved specificity through extensive sequence similarity, but divergent function through their appended catalytic modules.
pfam13229	Beta_helix	1.52e-11	374	555	5	157	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
pfam13229	Beta_helix	2.49e-10	397	555	4	133	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
pfam12708	Pectate_lyase_3	8.12e-07	226	446	3	194	Pectate lyase superfamily protein. This family of proteins possesses a beta helical structure like Pectate lyase. This family is most closely related to glycosyl hydrolase family 28.
pfam07705	CARDB	5.11e-06	791	904	3	100	CARDB. Cell adhesion related domain found in bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUB45559.1	0.0	41	926	28	917
ANR73273.1	0.0	41	926	27	916
QUB81474.1	0.0	35	942	19	933
QUB58650.1	0.0	41	929	28	920
QUB56055.1	0.0	41	929	28	920

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5ZRU_A	4.61e-163	53	601	13	565	ChainA, Alpha-1,3-glucanase [Niallia circulans],5ZRU_C Chain C, Alpha-1,3-glucanase [Niallia circulans]
6K0M_A	3.24e-30	49	524	11	461	Catalyticdomain of GH87 alpha-1,3-glucanase from Paenibacillus glycanilyticus FH11 [Paenibacillus glycanilyticus],6K0N_A Catalytic domain of GH87 alpha-1,3-glucanase in complex with nigerose [Paenibacillus glycanilyticus]
6K0P_A	3.23e-29	49	524	11	461	Catalyticdomain of GH87 alpha-1,3-glucanase D1045A in complex with nigerose [Paenibacillus glycanilyticus]
6K0S_A	3.23e-29	49	524	11	461	Catalyticdomain of GH87 alpha-1,3-glucanase D1069A in complex with nigerose [Paenibacillus glycanilyticus],6K0V_A Catalytic domain of GH87 alpha-1,3-glucanase D1069A in complex with tetrasaccharides [Paenibacillus glycanilyticus],6K0V_B Catalytic domain of GH87 alpha-1,3-glucanase D1069A in complex with tetrasaccharides [Paenibacillus glycanilyticus],6K0V_C Catalytic domain of GH87 alpha-1,3-glucanase D1069A in complex with tetrasaccharides [Paenibacillus glycanilyticus],6K0V_D Catalytic domain of GH87 alpha-1,3-glucanase D1069A in complex with tetrasaccharides [Paenibacillus glycanilyticus]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.009818	0.987680	0.001088	0.000541	0.000411	0.000428

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000139_00209.