CAZyme Information: MGYG000000139_00217

Basic Information

• Species: Eubacterium_G sp000434315
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eubacterium_G; Eubacterium_G sp000434315
• CAZyme ID: MGYG000000139_00217
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1808		201714.32	4.3956

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000139	2758071	Isolate	United Kingdom	Europe

• Gene Location: Start: 263903; End: 269329 Strand: +

Full Sequence      

MRTKHYRMIKKSLACALSMAMLAGSADFSNLHVSAADRSKPWLLSTNRPAYASSLNGGDV
ASFATDGKISTQWGVAANKANQWLDIDLGGKADISKVVIDWQNDASYGVAYQVLVSDDEI
NWTKIYETTSGNGGEVKNVLKKDGTVDYSYYEDVLSTDATEDYKLTKGNGRYVRILINYS
KSQSGSDDKKSGWGASIREVGIYGIGDDNCIAPVSEAENIALGKEVSVSSYSQPWWASTP
LSGSNAVDDDYDSYWLSQSYDSVAQTADQNLTVDLGKEYTVGRVVLQWQTEFARIYDLQV
SADGNNWNTVYRNIRGNGEDDDISFYAENVRYVRMQGIAYGRGSGYSIREIKVYEYQNGD
EKVVNQIPDLPKKEVVKLGKGSYAIDDTNLLQPRDPKYVTSNVKSAIPSNDWWTSIVYTQ
YSDTMPALPLVYSYSATGLGLYYADGFYTRSDNGGMGAESKYKDITIGTSAVTKEASAKL
HSYGDWSVDVAYTDTDSPKMLNTLIKGSPYVYSTFEDASSVEINVANLVKFFDKDGKEVL
KEDSETLKGDQFGVEAKNESEAPGDGNKTQYHYYGVYMPAGSTVTRVGSKLKISLGDNQN
YMVVGALAVDEPIKLLATQKEAAKVHNPESAKDAEAVAQLAYMYKHAYSYVTDTKVTATY
DESKAVNTTKYESVISQKRNDNGIENSTLMCMMPHQWKYSDASYKKAESGKALIYNSVRG
DLRIHEGNEFNYTQKFNGIIPQYTTPDESGSYDTEWMYAYLKQFTDSALKSYWVADPYWQ
GKKSHPITMGILIADQLGEYETRDKLISVLRKIMENWLTYDGEEDFPYYMYYHTSWGAVS
GDGGDHGMAINLSDHHFLWAYFIFPAAVLASYDSQFVEDYGDMVELLIRDAMNPDKDDEL
LPYMRNFDVYEGHSWAGGYGDNNSGNNQESASEATFAWAGLYLWGLVTENNTYRDAGIWG
YTSEVDAIEQYWFNMDAGTELDSNNWAPGYGTDVYGDEDCDVLPYTGMVWGLGYTNGTYF
SGNPCCIMGIHLLPVTPAIIYMGYRKNTVDNIWNQYEQIQKAYQKKLEKNGESDPEGWYH
ILWPFMALSDADGAAGRWANEYTSHLNEDGNYIDGVLATDEMFNSYWYIQNMCAKGYIDT
SIWSTDYTSYQVFSKTANGKTTYTAEVWNPFDKEISVHFANANGNLGSAKVPAHSTVDVD
PTKNEDKTIDENGDKVTYVPVQNTDPVNEVSGVIEAEDYYTNFSCEPDTNDQEGGYVGWI
DDGDSLIYTINVKEDADYVVDYRVQCVNTEKSSAIKLKTDNDSDYILTTYLDNSKTDWKN
VKADKTIHLKKGEYKMKLKFVDGGFNLNYIKIYKKGTKAPVAASDDVTFADLSNYPEIDL
SKAEVIDVTSEANADGKASNVIDKDYNTRWESKAEDPQSITIDLKENKEIGGIKLYWEGA
YAKKYEIQVSSDNTNWKTMFTQNKGIGGQKNGDSNRSTGLESISFDNAVNGRYVRIKCIE
RGTGYGNSLYEIRLFGKGEEQGGVTEPEQPTQPAEPDTPDGNLALNKEVSSSAPEGENVV
AANAFDGNDGTRWSSKFEDDAWISVDLGKTYTVGKVVLKWEAAYGKAYDIQVSTDGKEWT
TVKKMRNQDGGEDTASLNGTKARYVKLQGVERGTGYGYSLWEMEVYGGKVSQGETNTPSG
TNVALNADTKQSGSENDAMTSEKAVDGNGESRWSSNYADDAWMTMDLGNAYEINKVLINW
EGAYGKNYDILVSMDGENWTTVKEMRNQNGREDTVEFNAVKARYVKFQGVERGTGYGYSI
WEMQVLTK

Enzyme Prediction     

No EC number prediction in MGYG000000139_00217.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH81	395	1110	1.6e-115	0.9726688102893891
CBM32	1550	1664	2.6e-29	0.9274193548387096
CBM32	1688	1804	5.7e-29	0.9516129032258065
CBM32	1389	1512	5.3e-26	0.9032258064516129
CBM32	236	352	3.4e-22	0.8951612903225806

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5498	Acf2	8.86e-64	376	1114	45	740	Endoglucanase Acf2 [Carbohydrate transport and metabolism].
pfam17652	Glyco_hydro81C	8.33e-38	793	1115	48	341	Glycosyl hydrolase family 81 C-terminal domain. Family of eukaryotic beta-1,3-glucanases. Within the Aspergillus fumigatus protein, two perfectly conserved Glu residues (E550 or E554) have been proposed as putative nucleophiles of the active site of the Engl1 endoglucanase, while the proton donor would be D475. The endo-beta-1,3-glucanase activity is essential for efficient spore release. This entry represents the helical C-terminal domain.
pfam00754	F5_F8_type_C	2.27e-22	1548	1662	1	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	1.24e-21	1386	1511	2	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	9.58e-20	1690	1802	3	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADQ45030.1	0.0	42	1805	40	1638
BCJ99064.1	0.0	42	1352	33	1242
AIQ32147.1	4.23e-283	42	1805	31	1510
ANS76332.1	5.49e-264	19	1805	20	1661
QZN75609.1	1.34e-255	33	1805	37	1654

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6FOP_A	3.24e-92	379	1181	6	700	ChainA, Glycoside hydrolase family 81 [Acetivibrio thermocellus ATCC 27405]
5V1W_A	6.10e-73	376	1195	4	746	ChainA, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]
5T4A_A	8.03e-73	367	1195	15	766	ChainA, Glycoside Hydrolase [Halalkalibacterium halodurans C-125],5T4G_A Chain A, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]
5UPI_A	1.52e-72	376	1195	4	746	ChainA, BH0236 protein [Halalkalibacterium halodurans C-125],5UPM_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125],5UPN_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125],5UPO_A Chain A, BH0236 protein [Halalkalibacterium halodurans C-125]
5T4C_A	1.96e-72	367	1195	15	766	ChainA, Glycoside Hydrolase [Halalkalibacterium halodurans C-125]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9UT45	2.89e-22	689	1114	321	722	Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1
P53753	1.21e-21	787	1090	797	1077	Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1
D4AZ24	1.04e-19	579	1106	371	907	Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1
Q09850	1.67e-16	778	1110	379	689	Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1
Q5AIR7	1.23e-13	834	1114	872	1126	Endo-1,3(4)-beta-glucanase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ENG1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000781	0.994073	0.004292	0.000375	0.000240	0.000187

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000139_00217.