CAZyme Information: MGYG000000142_01286

Basic Information

• Species: Blautia_A massiliensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A massiliensis
• CAZyme ID: MGYG000000142_01286
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
628		72627.35	6.8494

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000142	3538467	Isolate	United Kingdom	Europe

• Gene Location: Start: 49372; End: 51258 Strand: +

Full Sequence      

MGKKLQFTELDQYLFGQGTNYDIYRKLGAHPDTQNRKKGVYFAVWAPNAQAVSVIGEFNE
WDTEKNPMKKVGPIGVYETFVPGAKIGQLYKFYITGAHGEELYKADPYANAAEMRPGTAS
RITDITNYKWKDDTWMKNRENFDPDTSAMSIYEVHPGSWMKHPQTAEDEDGFYSYRELAP
KLAQYVKKMGYTHVELMGIAEHPFDGSWGYQVTGYYAPTSRYGTPQDFKYLVDYLHRQKI
GIILDWVPAHFPKDAHGLVNFDGTAVYEHADPRQGEHPDWGTKIYNYGRPEVKNFLIANA
LYWVEECHVDGLRVDAVASMLYLDYGKKDGEWIANKYGGNKNLEAIEFFKHLNTVVLGRN
HGTVMIAEESTAWPMVTGKAEDDGLGFSLKWNMGWMNDFLEYMKLDPYFRKFNHNKMTFS
MTYAYSEKYVLVLSHDEVVHLKCSMLEKMPGELDDKFKNLKAGYTFMFGHPGKKLLFMGQ
DFGQLREWSEARELDWYLLGEENHQKLQQYVSDLLHIYRKYPALYGADNDPSAFEWINAN
DGDRSIFSFVRKSPTRRNNILIVCNFTPVARPDYRVGVPKKKQYKLIMDEQGLLPKGKVF
KAEKKNCDNREFSFAYPLEAYGVGIFTY

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	184	484	4.3e-152	0.9966777408637874
CBM48	26	112	9.6e-21	0.9078947368421053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	6	626	6	625	1,4-alpha-glucan branching protein GlgB.
PRK14705	PRK14705	0.0	9	626	605	1220	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	8	613	7	613	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14706	PRK14706	0.0	27	588	27	576	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	6	588	99	677	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCU01659.1	0.0	1	628	1	628
CBL22762.1	0.0	1	628	1	628
AWY99231.1	0.0	1	628	1	634
QIB55783.1	0.0	2	628	4	636
QMW76350.1	0.0	2	628	4	636

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GR5_A	6.43e-230	8	584	130	723	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	5.19e-229	8	584	130	723	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR2_A	5.19e-229	8	584	130	723	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQW_A	5.19e-229	8	584	130	723	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQU_A	7.35e-229	8	584	130	723	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	1.13e-291	1	628	1	632	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q2JT08	1.97e-238	9	593	119	712	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-3-3Ab) OX=321327 GN=glgB PE=3 SV=1
Q8DLB8	3.97e-237	6	585	105	701	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
Q2JK68	3.32e-235	9	593	119	712	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=glgB PE=3 SV=1
Q1AZ86	7.75e-234	8	589	100	675	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000039	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000142_01286.