CAZyme Information: MGYG000000144_00895

Basic Information

• Species: Barnesiella intestinihominis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Barnesiellaceae; Barnesiella; Barnesiella intestinihominis
• CAZyme ID: MGYG000000144_00895
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
856	MGYG000000144_2|CGC1	97653.25	6.6366

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000144	3314966	Isolate	United Kingdom	Europe

• Gene Location: Start: 64656; End: 67226 Strand: +

Full Sequence      

MKRFITGSSLILVSILSGFPVQAGKSLDEIDRESFAAKYILSELPGISMIPVSSVDTVRN
REYFSLDGTWLMAEGGDEKFRLKDVWTDAVEAVVPGSVHTALLMNRIIPDPYIGQNDSIA
EQQSYKTWWFSKKFTLNELLENPILSFGGVANRCTVWLNGKKLGSHEGMFGGPDFVVKKY
LKKENTLVVKLDPIPQMFLGNWPPNANESWKYTVVFNCVYGWHYAQIPSLGIWRSVKLES
QAPVEIEEPFVSTRSLQGDMRLAVTLKKINAPLKGKLQIRVVPKNFEGAPQFFEHEINSS
KKTEQFSFDFKIDAPRLWWPNDMGEQSLYDMTVAFIPRNGARPDVEKTSFGIRTIDMAPF
PGGEREDRYNWTFVINGKPMFVKGTGWCTMDALMDFSREKYDRLLSIAKSQHIQMMRAWG
GGLPETDDFYELCDKYGIMVIQEWPTAWNSHNTQPFDMLQETVVRNMKRIRNHPSLVMWG
AGNESDKPFGPAIDMMGRLSLELDGSRPFHRGEAWGGSDHNYNCWWDDAHLNHNLNMTSP
FWGEFGIASLPHIESVRRYLAEEKDRWPSRPGDHFRHHTPIFGTMGEFGKLSQYSGYFMP
DTTLAEFITGSQLAQVVGVRHTLERARTLWPETTGALYYKMNDNYPGVSWSSVDYYGAIK
PVHYFVQKSFAPLTGVLLFDRTNLSSQEVSLPLYLLDDCSQLNGKDYTVSVTVYNDRLDT
VVCREFDGHGELETVKKLGNLNLNRAQTKSTMLFFVVDVCSEGKRLSRNYYFTNYEVRRG
VIMSMPRTEITMKRDGRQITVTNVGKLPAIGVYVESPGYAHEFIASKNYLWLDPGESQII
EVNVDYPVCVKGWNIN

Enzyme Prediction     

No EC number prediction in MGYG000000144_00895.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	58	714	2.9e-90	0.6622340425531915

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	6.76e-44	76	664	17	624	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	1.18e-18	61	508	10	444	beta-D-glucuronidase; Provisional
PRK10340	ebgA	1.71e-18	125	510	113	473	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02836	Glyco_hydro_2_C	8.34e-13	373	510	8	159	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK09525	lacZ	3.55e-09	231	519	208	493	beta-galactosidase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SCV07058.1	0.0	11	855	8	852
QRQ54566.1	0.0	11	855	8	852
ALJ47660.1	0.0	11	855	8	852
QUT26201.1	0.0	11	855	8	852
QUR45526.1	0.0	11	855	8	852

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5N6U_A	8.08e-72	62	772	25	745	Crystalstructure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_B Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_C Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12],5N6U_D Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum. [Dictyoglomus thermophilum H-6-12]
2VJX_A	1.15e-62	62	852	7	824	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	1.17e-62	62	852	9	826	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	1.19e-62	62	852	9	826	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	2.92e-62	62	852	7	824	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q95327	3.02e-49	65	690	24	708	Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=1 SV=1
Q29444	4.22e-48	65	684	24	702	Beta-mannosidase OS=Bos taurus OX=9913 GN=MANBA PE=1 SV=1
Q4FZV0	1.83e-47	64	676	23	694	Beta-mannosidase OS=Rattus norvegicus OX=10116 GN=Manba PE=2 SV=1
Q8K2I4	3.25e-47	64	676	23	694	Beta-mannosidase OS=Mus musculus OX=10090 GN=Manba PE=1 SV=1
Q75W54	1.05e-46	90	690	43	710	Mannosylglycoprotein endo-beta-mannosidase OS=Arabidopsis thaliana OX=3702 GN=EBM PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000977	0.897880	0.100292	0.000322	0.000260	0.000222

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000144_00895.