CAZyme Information: MGYG000000148_00758

Basic Information

• Species: Faecalimonas phoceensis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Faecalimonas; Faecalimonas phoceensis
• CAZyme ID: MGYG000000148_00758
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1707		188751.76	4.5336

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000148	2855435	Isolate	United Kingdom	Europe

• Gene Location: Start: 179672; End: 184795 Strand: -

Full Sequence      

MKKKQVVSGLLATVMIANIALVDMGPVQAAENLNLAKGCNVTASSYEVSDQIDTSPAKAV
DGDMATRWGTAQNKADNEWIEIDLDGEKTVRQININFERTDEAQNILSYKIELEENGKYT
EVYKKTAKAKQKEVIQLDKNHKATKVKVTVLSADQGTINWKNVGINEIEVYSELVEVADA
TPNKNHMANATVTASSVEKNTQFTADKVKDGKTDKANRWASDYETPTTNIWLNSKFAGLT
SVKEIKIHFLERDVAPQPSNVKSFDIKYIDKNGEEKTAKSFTNTKQGEGYKNEVVIRLDQ
PIEAKELKLCNFVAEAAEWNNIGILEMEVYSNDQAEQGATLDSVVEAIEAESKTIAADVD
TLEMPVVPAGFSVKLNGADFEQIIGDNGKIVHPLTDKTVKVSYVVTETATGKGKETKDVD
YIVKGTKTQADGKNAKPTVIPEIQEWYSDSTEKIAVSSLKTVTYTDDKLKDVVDEFVSDY
EDFTGIKLTAKKGGAEANAFNFELKAPDELLGEEGYTMDIQKDRINVASVDTTGNMYGMQ
TILQMYKENNERYNVGQMRDYPRFETRGFLFDVARKPVSLEMMKEVTRTMRYYKMNDFQA
HLSDNYIFLEDYGKGAQENEAFKAYEAFRLESGLSNEKGETPTAKDYSISKKDFRQFIQD
ERALGMNIVPEIDVPAHATSFTKIWPDLMVKNKVSSLNANRPLVDHLDVSKTETKEKIKE
IFDDYTKGDNPTFDSETTVHIGADEFLADYKAYRGFVNDLVPHVKETNTVRMWGGLTWIK
DNPVTEIDKEAIENVEMNLWSADWADGIEMYNMGYDLINTIDNFGYMVPDGSKARANAYG
DLLNVERIFNEFEANKVRVKGGAYKYVPAGDDQMLGAAFALWSDNIDKRASGLSESDLYW
RFFDALPFYAEKTWAATGKEKGSADALAKLATDKGTGPNTNPYYQEDKKGENYESYDFED
GLKDGSENKRDLKEGKNAEVKENALVLKDGESYVTSPIEELGNGNQLSFDIKLEEPAKPG
DILFESDAAYGTHDIRIMEDGKLGFTRELYNYYFDYELPVGKNVTITITTDQQKTSLYVN
GEFVADAKGKFIHNDMVKKENITNATFALPLERIGSKTETVSAVIDNVVVSAKEPEQDIY
NKAAWTGTAESETPTSQGGGKEGVIGMAFDNSSSTHWHSDWSQATKDKVPTSAGAAGTGK
GADGSIWAEVKFDKEYEINQVLFAPRQDTNSGQVTKASLYIQTEKDGEWVEVAKDKTFAA
DKSEKAFTFDKQMVYGFKFVATQSNDGWVTVSEFNIADKKVTDKIYTVYVEAEKGGKVSG
GKDVSAGESVTVTATPDKGYAFEGWYRPNGEKVSEKAEYTFKVNGNTALIAKFEKTGETE
DKNITDVEKLDDITVVQGTAFEDLDLPEKVSVTYGKNETAEVDVTWEKGTYNAQECKTYV
LEGALTLPEGIANPDGLKAIVKVTVEEKETPVKKYTVSVNVKDSEKAMGTVSMDKEDGVY
EDGMNATVTATANEGYEFVNWTDTDGKEVSSSNPYTFAVTANTTLTANFKKMDVKPEAPT
AQDILDEIIAGQKVPTVITKGETELVLPKVPEGYKIQITKVNPEGIIALDGTVTTPKKDT
DVIVTISVTDPEGKTASADFTVKVKGKNEPGKEDPKDPQNPSKGDTNKKDPSQDKKPVKT
GDATAAAGWMLAMTAAGAVVLVRKRNS

Enzyme Prediction     

EC	3.2.1.52

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH20	560	916	5.4e-52	0.9703264094955489
CBM32	42	168	2.4e-19	0.9596774193548387

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06564	GH20_DspB_LnbB-like	4.20e-113	565	915	1	325	Glycosyl hydrolase family 20 (GH20) catalytic domain of dispersin B (DspB), lacto-N-biosidase (LnbB) and related proteins. Dispersin B is a soluble beta-N-acetylglucosamidase found in bacteria that hydrolyzes the beta-1,6-linkages of PGA (poly-beta-(1,6)-N-acetylglucosamine), a major component of the extracellular polysaccharide matrix. Lacto-N-biosidase hydrolyzes lacto-N-biose (LNB) type I oligosaccharides at the nonreducing terminus to produce lacto-N-biose as part of the GNB/LNB (galacto-N-biose/lacto-N-biose I) degradation pathway. The lacto-N-biosidase from Bifidobacterium bifidum has this GH20 domain, a carbohydrate binding module 32, and a bacterial immunoglobulin-like domain 2, as well as a YSIRK signal peptide and a G5 membrane anchor at the N and C termini, respectively. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself.
cd02742	GH20_hexosaminidase	4.57e-28	567	914	2	302	Beta-N-acetylhexosaminidases of glycosyl hydrolase family 20 (GH20) catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues from the non-reducing ends of N-acetyl-beta-D-hexosaminides including N-acetylglucosides and N-acetylgalactosides. These enzymes are broadly distributed in microorganisms, plants and animals, and play roles in various key physiological and pathological processes. These processes include cell structural integrity, energy storage, cellular signaling, fertilization, pathogen defense, viral penetration, the development of carcinomas, inflammatory events and lysosomal storage disorders. The GH20 enzymes include the eukaryotic beta-N-acetylhexosaminidases A and B, the bacterial chitobiases, dispersin B, and lacto-N-biosidase. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by the solvent or the enzyme, but by the substrate itself.
pfam02838	Glyco_hydro_20b	4.55e-27	436	560	1	123	Glycosyl hydrolase family 20, domain 2. This domain has a zincin-like fold.
COG3525	Chb	1.92e-22	510	935	202	630	N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism].
pfam00728	Glyco_hydro_20	1.62e-20	564	916	1	345	Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAI94822.1	0.0	4	1375	7	1373
ADO53681.1	0.0	4	1375	7	1373
QTQ18922.1	0.0	4	1375	7	1373
BBA57859.1	0.0	4	1375	7	1373
VEG17438.1	0.0	4	1375	7	1373

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JQF_A	1.98e-95	360	1116	5	720	Crystallizationanalysis of a beta-N-acetylhexosaminidase (Am2136) from Akkermansia muciniphila [Akkermansia muciniphila ATCC BAA-835]
7DUP_A	5.04e-16	460	745	29	316	ChainA, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVA_A Chain A, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVA_B Chain B, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron]
7DVB_A	1.54e-15	460	727	29	303	ChainA, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVB_B Chain B, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVB_C Chain C, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron],7DVB_D Chain D, Beta-N-acetylhexosaminidase [Bacteroides thetaiotaomicron]
6YHH_A	3.93e-13	510	745	88	317	X-rayStructure of Flavobacterium johnsoniae chitobiase (FjGH20) [Flavobacterium johnsoniae UW101],6YHH_B X-ray Structure of Flavobacterium johnsoniae chitobiase (FjGH20) [Flavobacterium johnsoniae UW101]
7CBN_A	2.78e-12	508	688	72	252	Crystalstructure of beta-N-acetylhexosaminidase Am0868 from Akkermansia muciniphila [Akkermansia muciniphila ATCC BAA-835],7CBO_A Crystal structure of beta-N-acetylhexosaminidase Am0868 from Akkermansia muciniphila in complex with GlcNAc [Akkermansia muciniphila ATCC BAA-835]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B2UPR7	1.35e-100	355	1116	34	742	Beta-hexosaminidase Amuc_2136 OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_2136 PE=1 SV=1
Q9HGI3	7.09e-14	513	923	128	554	Beta-hexosaminidase OS=Emericella nidulans OX=162425 GN=nagA PE=1 SV=1
P43077	2.56e-13	514	922	112	513	Beta-hexosaminidase OS=Candida albicans OX=5476 GN=HEX1 PE=1 SV=1
E9DFH0	3.64e-13	513	827	121	441	Beta-hexosaminidase 1 OS=Coccidioides posadasii (strain RMSCC 757 / Silveira) OX=443226 GN=HEX1 PE=1 SV=1
P13670	2.62e-12	509	714	276	489	N,N'-diacetylchitobiase OS=Vibrio harveyi OX=669 GN=chb PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000205	0.999132	0.000165	0.000173	0.000154	0.000140

TMHMM  Annotations     

start	end
7	29
1685	1702