CAZyme Information: MGYG000000150_06216

Basic Information

• Species: Hungatella sp005845265
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Hungatella; Hungatella sp005845265
• CAZyme ID: MGYG000000150_06216
• CAZy Family: GT2
• CAZyme Description: Plipastatin synthase subunit D

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2643	MGYG000000150_49|CGC1	301065.86	4.8559

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000150	7470188	Isolate	United Kingdom	Europe

• Gene Location: Start: 26993; End: 34924 Strand: -

Full Sequence      

MRQNGSIMEIFHNFFNNSENEIIFYDLSEERFSADNLRNMVEKTMAKLSSVERREYVILQ
IGDKKEFLISLISLFCLDAIPVILPMCTSQSGYEQLKKILKAHPGTLVIADNQGSQNLAH
FTDGSINIQNIEADAMSLCRRGRAYGEQDEAIIMYSSGSTSEPKGTILTVENIIESVKEK
SIEYSVAQNDVFMTWMSLEHIVGMVDFMFLPFFTGCTCVYSEISSFLSNPREWFDLMEKY
NVTVSVAPNFAYKYMSDCITDSDNWDLSSLRILMSIGEPISRQVIQRFFSKVNRFHIDEE
KFVAAYGLSETCSGVVVNKGNVLKAKVAPEVNITSGIDFDSSHFDYKKADLICQGEPIAD
TKVRIMDDHREELKENCIGNIEIKGSAVCKGYFEYDGRQPVEDGWLSTGDIGFYSGGALY
VIGRRKDVIFSYGKNIYLSDLEYAVAKYFQLRSVACGDNMSSEGEFYIYLFVEINEAEGQ
QMKSKIRHTILRELGIKLDDIIFMDKLSNTKAGKISKAEIMRKYTMSRDSERRDNIEGFI
KGYFAGEVTEDTSILDVAEDSLSMFRLIGAIHKQFGIKMGILDLTRCETIHDLCDYINKL
SGREIPVEKITCENQMFCTSIQEAYILGRQQEFYGNTNMSHFYLEVRHNLSSQKIEEAIR
KLIRRHSMLRSIFENSRRVVLGEDIADTFFLKTLEEKSLESHRKAANQKVNEPDKWPLFE
ICNIKEGDSYIAAIDIDMLIVDGFSLSVLVRDFKSLCRGLELEPISDGFGKYLEAYSIRK
SGNRYLQDKQYWLERVKTLPGSPQIPILAAVNRGPNVFLRKEKAFTEQAWEELENVAERN
GVTISVLLLTLYIHTLKRWSEEPEFTLNVTVMDRPTQMKDTERFVGDFTTNTLFNYENES
GSPRNLYQDLSHVKGRLYEYLDHNNFDGVEVIREYAKVHQGEMALMPVVFTSMLFKEDNA
LTYEDINYFVTQTSQVYLDCQIYHYDTTYVIAWDYLEKVFPDNMIHNMFDYYMNLVEGII
CGREINEIPVGNERAVTRYNETDYSEELMTIPEMLKRSLDFFGPKTAITDGETVLTYEQL
KRRMEDMIEDYIKQGMKCGDTVMVLAGKDIDSVVAILAVAKIGATFIPVPEDYPKERIET
IREYSSAEWIVHVQNNKIERCVSAKRQRNHTTSKLEDTAYIIFTSGSTGNPKGVAISHEG
VMNTLVDMKKRFALDENEGVLGLSALNFDLSIFDIFGSIYMGGFLSLVKDPRNAEEINHM
LEQFPITIWNSVPAIMKLFLESLPVDYRNTEINHIFLSGDWIGTELPEKIKKVFTNAEII
SLGGATEASIWSIYFPIREVRKEWSSIPYGYPLGNQKIYILNKEGDACPTNVVGEICIGG
RGVALGYVGDEEKTNRSFVDIPSIGRVYKTGDYGKFSEEGYVIILGRKDGQVKINGFRIE
LGEIEAVARKFYTVDNAIAIMDSKKKLALFYTGKEIEDRELQVHFEKYLPAYMIPYRFVY
MEQFPLSKNGKIDRAALLQIRELKEESEISAPANEIEMKISVIWGKYLESDQVSVDENFF
ALGGDSIIAQRIAQELTKQFAVKIPFVKIVETGTVKELARFIQAETESKEEPKATLLSEN
QGEKTVEASQTELPMTNIQKAYFNGRNDSFELAHYNAHYYFEVEFPYEISDIERSIAQML
ERHEVLRSIFLKNGTQKILADVPEYKVDITIAETEQEFAEKNEQMRRELSHKNYDFSKWP
LFTFEVLKRGCDDFKILCASINLMVCDGDSLRIFLKEFTEGLNNKKLPPIHYSYHEYVNS
LNDCTNEREYQEAKAYWLNKIPCFPEFPHLPMLKNLTQCKNYRISRVSETIDAETWGTFK
NTVRKHAVSPSTVLCTIYARVLAKWSNQKDLLLNMTAFERKEFDQNVSGILGDFTKILPL
EVHIDGADFWDNAMQIQTRILNNLDHKAFDGTEIIRELSRLSNGIGKALMPVVFTCVLFD
SDENWFEQIGKLRYAVTQTPQVFLDNQILEMKHELYISWDYVEDLFNPAIIAAVFEDFIS
GIKNIAVNGVFETRSQIETDKVWDGYNMPVERVAPNTLQAFFEKQVRETPDGIALYYGDK
QYTYAAIEAKANQVARYLCSHGICGANNRVGVLGERKPETIISIIGVLKTGAAYVPVDAK
FPKERRDFILENSGSRLLLSEELYRSGELEQYDESPIGIQSLPDSLVYIIYTSGSTGKPK
GVMINHMAACNTISDLNNKIKLNKTDRLIGISSVCFDLSVYDIFGAFSTGAALVMVRDAR
DCSEIVSLLAERKITVWNSVPVICSIVVQHMLERGMKDSLSLRQIMLSGDWIPLDLPDKA
KSIFNKTKIMSLGGATEASIWSIYYEIGEVKPAWKSIPYGMPLKNQTMYVLNFAGELCPM
DVVGEIYIGGVGVAQGYCGDTEKTEAAFIQHDHLGRIYKTGDMGVLRKEGYIEFLGRIDN
QVKIRGYRIELGEIESAINNVPGIEKSVISYVVNRTGNKQLIAYYIPTSNDLASETIKES
IGAFLPEYMVPQHYISIKELPLSANGKIDRSKLPELNLDKKERALSKDVISPLQKTLLGI
WQKVFENTEITITDDFYGLGGDSIVLMKLLDEIALAGLENISIEDILEYETIEALSAYME
ENK

Enzyme Prediction     

No EC number prediction in MGYG000000150_06216.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12316	PRK12316	0.0	696	2636	1646	3619	peptide synthase; Provisional
cd12114	A_NRPS_TlmIV_like	0.0	2090	2553	1	477	The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
PRK05691	PRK05691	0.0	149	2637	167	2773	peptide synthase; Validated
PRK12467	PRK12467	0.0	644	2637	78	2161	peptide synthase; Provisional
cd12114	A_NRPS_TlmIV_like	3.57e-156	1065	1517	3	477	The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QND46664.1	3.04e-159	130	2643	62	2666
BAY30132.1	1.08e-93	1811	2636	313	1177
BAY90071.1	3.15e-93	1811	2636	312	1174
BAZ00088.1	1.06e-91	1811	2636	313	1175
BAZ75991.1	1.06e-91	1811	2636	313	1175

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LY7_A	1.72e-250	1635	2617	13	995	ChainA, BmdB, Bacillamide NRPS [Thermoactinomyces vulgaris]
7LY4_E	1.78e-250	1635	2617	13	995	ChainE, BmdB, bacillamide NRPS [Thermoactinomyces vulgaris]
6MFZ_A	5.18e-174	1010	2639	173	1797	Crystalstructure of dimodular LgrA in a condensation state [Brevibacillus parabrevis],6MFZ_B Crystal structure of dimodular LgrA in a condensation state [Brevibacillus parabrevis]
6MFY_A	2.32e-166	1010	2555	173	1714	Crystalstructure of a 5-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis],6MG0_A Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis],6MG0_B Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis]
6LTA_A	1.40e-130	1634	2613	58	1072	ChainA, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23],6LTB_A Chain A, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23],6LTC_A Chain A, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23],6LTD_A Chain A, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23],6LTD_B Chain B, Nonribosomal peptide synthetase [Streptomyces sp. Sp080513GE-23]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O68006	4.07e-298	1041	2639	30	1651	Bacitracin synthase 1 OS=Bacillus licheniformis OX=1402 GN=bacA PE=3 SV=1
P94459	2.53e-208	355	2642	778	3107	Plipastatin synthase subunit D OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsD PE=1 SV=2
P0C064	3.38e-203	149	2613	1647	4134	Gramicidin S synthase 2 OS=Brevibacillus brevis OX=1393 GN=grsB PE=1 SV=2
P0C063	5.77e-199	149	2613	1647	4135	Gramicidin S synthase 2 OS=Aneurinibacillus migulanus OX=47500 GN=grsB PE=3 SV=2
P39847	1.50e-196	728	2643	135	2077	Plipastatin synthase subunit C OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsC PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000051	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000150_06216.