CAZyme Information: MGYG000000152_04341

Basic Information

• Species: Lacrimispora sp902363835
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lacrimispora; Lacrimispora sp902363835
• CAZyme ID: MGYG000000152_04341
• CAZy Family: GH28
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2015	MGYG000000152_23|CGC2	218820.94	4.3776

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000152	6260407	Isolate	United Kingdom	Europe

• Gene Location: Start: 48859; End: 54906 Strand: +

Full Sequence      

MNAILKRCRKALAVFLAVAMLPQFGLAGGGTAYAELSSEPDGIRVATPSEAAATPSNAND
SQGYGTVSLLETVATPSVAAATPSNAELLLAGESRYSRSEGSSDVTNVRVPELAYDNTSI
TLVWDKPENYGDVANYDVYCDDVKIGDARSNFAEHADWANTYMNAFYNYYDAQGIDMVQA
DIHTFRADGLTPDTSYTFSVVAVDAHGTELGDRASATWSTTGTPEEFHIMDYGAEPVEAG
FTSSDEDRDAFIVKNTKAIQSAIDACSEGGKVVIPAGIYMSGSLYLKSNMTLELEEGAVL
FGSPNVNHYDQNYLLYPYSTDTRSWALINAYSADEHGGFENIRIVGKGTIYGNGWKYGSG
STISGDGYSPAYQKKQSGDPDWTKAELQKYALKNWVAGNNSKVSGNSNFGILAKSAFDMG
RGKGLTDSEAYAMRPNLVVLRGVNGVYIQGITVENPANHTIAVLDSENVVSENVKYITYD
ANNGDGIELGNTQNAMVFNNFFDTGDDAVNFATGMGKGVQDTEQKPSGNIWTFNNFLREC
HGGAIAAGSHTGAGIGQMLVEDNVLNHSDMPFRFKSAPVNGGSVSDILIRDCAVGDAQQV
FTFSTSYSDANQAVTVEPADKPAEFYQVDAYNITADTITKNTFEIAASVDYEKMYQPWHT
HHDLYFQDITFTNIAASLAKTSEKLEGVSNTVFHNVVMNWKGIEKKDNDAAATAYKAWGS
IKFSNDLSFTGTTTQSTASYDAMAKPVWPEDVLTVASASEASKGGSERRVKLKWPAAEDG
EQETGSGGVTGYIVETYLNDELIDVTKPVTTTSCELGGLSPDVAYVFKVYAVDAPGNKVL
GLQYDWTTSEGEDREVKEPVDGKITFSGIGYTWGTAKFKSAKAADSRIRGYRAYVNGELA
ATVYNYQLKEPEAEEITMTIGRMLEDSNDVYLEAFTDSDQTYEYKEATVKTTKAYDYLAP
VWKSDELKVSLSGNDFILSWEEPEDESGIYGYRVYADGKPVYTTDGDYFNHVNGVYTTKD
TSFTVTGLDPEEQHTFRVEAADRWWKAINGSGPFHWTNSGPAVTWNGPEEELLWRSSAFG
QSTDLNFSSNVLDNKVGTNYTWPSGSDKPLDMEGKPVQDVVIESRGGKLQAGHDGVTFYY
TEVPTNKNFTLTADVTVEQMGPENGTNPNQQEGAGLMVRDVNGSPRKDPLEEGYEEYPAA
SNMVMLQAGSAGKSLNADLNLLAAARYGVNSPAGNLKSVKTTATFEKSAAKADKKYAPED
APYPSNFYGTQMKLRLERTDEGFTASHLDDGGNVISTYTFTDKNVTPNIVAHLDKDTMYV
GFFAARNARMSVKNISLVLSDVKQADESPAYVAPNDESASIYVASAAYTNGPAYTVQALT
NQSGRMTVSQNGAVIAANKEVTGGVQFTYPAVITGNEAEFKLSFVPDEGADTGKEKVQTF
KVKKEAYGDDLYAAPDGSDSGAGTMEAPMSVEAAVSRLVPGGTIYMAEGTYGPFQIPISS
SGNKDAGKALKAMGAVKITGSGKIFVLDSDYWDVTGLDVDGRNVEGSRGVMIHGSHNVMK
DSLIHNTSSDAGLTITKSRGSRLLWPSYNLIENCESYENIDGSRINADGFASKSGSGDDN
RFVNCVSHDNADDGWDLYNTLSDGPNGRTIIENCVAYNNGNNGFKLGGEGREVRSILRNS
VAFHNNLDGITDNFNPGELTIENNTSFDNRRFNIIMRPSPYKTDSDGNLTADGIVVNNVS
YRTSAYSASLDKVYDDKIAAREISNNYFYQNGANAITGDNFRSLDPAECFTRVDGKIVFG
DFLRPAKDSVIKMAGAGAQLPGINAPEPEPNPEPEPNPEPEPNPEPEPNPNPTPNPEPEP
EPGPNPAPVPGQNSDGSGSDSDKDYGIKKSSDETKGDWIKDTKGWRFKKQDGTYPKEEWV
KVSGAWYYFGLTGYMTTGWNKVGGIWYFMDQSGAMKTDWVLSEGKWYYMNLDGSMAAGWI
NVRNKWYYLSQSGECLMNTVTPDGYRVDENGAWVK

Enzyme Prediction     

EC	3.2.1.82	3.2.1.67	3.2.1.15

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL9	1448	1810	6.2e-95	0.984
GH28	265	676	8.8e-51	0.92

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5434	Pgu1	1.92e-63	108	591	1	392	Polygalacturonase [Carbohydrate transport and metabolism].
NF033840	PspC_relate_1	1.09e-28	1898	2015	512	648	PspC-related protein choline-binding protein 1. Members of this family share C-terminal homology to the choline-binding form of the pneumococcal surface antigen PspC, but not to its allelic LPXTG-anchored forms because they lack the choline-binding repeat region. Members of this family should not be confused with PspC itself, whose identity and function reflect regions N-terminal to the choline-binding region. See Iannelli, et al. (PMID: 11891047) for information about the different allelic forms of PspC.
COG5263	COG5263	1.59e-28	1898	2014	175	313	Glucan-binding domain (YG repeat) [Carbohydrate transport and metabolism].
NF033930	pneumo_PspA	6.44e-27	1898	2014	525	660	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
NF033838	PspC_subgroup_1	3.21e-24	1898	2014	548	683	pneumococcal surface protein PspC, choline-binding form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A. The other form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SET92437.1	0.0	1	2015	1	2000
QOV20136.1	3.39e-239	96	1060	65	1067
CAG77405.1	8.24e-155	1069	1804	40	742
QXE15447.1	8.24e-155	1069	1804	40	742
AIK16292.1	1.14e-154	1069	1804	40	742

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2UVE_A	2.37e-100	108	659	41	551	Structureof Yersinia enterocolitica Family 28 Exopolygalacturonase [Yersinia enterocolitica],2UVE_B Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase [Yersinia enterocolitica],2UVF_A Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase in Complex with Digalaturonic Acid [Yersinia enterocolitica],2UVF_B Structure of Yersinia enterocolitica Family 28 Exopolygalacturonase in Complex with Digalaturonic Acid [Yersinia enterocolitica]
1RU4_A	1.63e-23	1452	1709	18	292	ChainA, Pectate lyase [Dickeya chrysanthemi]
5OLQ_A	4.14e-15	1448	1822	2	430	Rhamnogalacturonanlyase [Bacteroides thetaiotaomicron],5OLQ_B Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLQ_C Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_A Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_B Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLR_C Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron],5OLS_A Rhamnogalacturonan lyase [Bacteroides thetaiotaomicron]
5OLP_A	2.14e-14	226	605	44	368	Galacturonidase[Bacteroides thetaiotaomicron VPI-5482],5OLP_B Galacturonidase [Bacteroides thetaiotaomicron VPI-5482]
3JUR_A	1.17e-09	256	609	44	364	Thecrystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_B The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_C The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_D The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P22751	2.87e-138	1073	1789	33	711	Pectate disaccharide-lyase OS=Dickeya chrysanthemi OX=556 GN=pelX PE=1 SV=1
P15922	3.87e-105	108	699	36	576	Exo-poly-alpha-D-galacturonosidase OS=Dickeya chrysanthemi OX=556 GN=pehX PE=1 SV=1
P0C1A7	9.32e-23	1412	1709	8	317	Pectate lyase L OS=Dickeya dadantii (strain 3937) OX=198628 GN=pelL PE=1 SV=1
P0C1A6	7.22e-22	1412	1709	8	317	Pectate lyase L OS=Dickeya chrysanthemi OX=556 GN=pelL PE=3 SV=1
P20041	3.77e-10	199	608	13	400	Polygalacturonase OS=Ralstonia solanacearum OX=305 GN=pglA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.002350	0.996320	0.000352	0.000364	0.000305	0.000271

TMHMM  Annotations     

start	end
12	34