CAZyme Information: MGYG000000153_01982

Basic Information

• Species: CAG-45 sp000438375
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-45; CAG-45 sp000438375
• CAZyme ID: MGYG000000153_01982
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
821	MGYG000000153_9|CGC3	95414.92	5.0123

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000153	2962513	Isolate	United Kingdom	Europe

• Gene Location: Start: 66783; End: 69248 Strand: +

Full Sequence      

MKAVTLNGAWKFGECGSVERYPVTVPGTVLSGLLDNGKIEDPFYRDNEYQTRELFWKDYE
FIRKFEVEEKLLSEEKILLVCEGLDTLADVYINDTKVLAADNMHRTWKVPVKSVLVRGTN
RIRIKFGSVLKFIENYPYRENREIRYVPCGAMKGNELIRKAHSMFGWDWGPQLIDAGIFR
DIYLEAYSGAKLDDVHIRQEHEAERVWLDVQTKLDGAAEDGARIRVSVEGEINTEIPADG
DGRVRLLLEHPKKWWPNGYGEQNLYTVTVELLAEDGSVRDVVKKRIGIRTLTISQEKDRW
GQEFAFIVNGVKIFTKGGNYIPEDCCYTRITKERQEYLVRSCVRANFNCLRVWGGGYYPN
DAFYDLCDEYGLIVWQDLMYACNVYDVTDAFAENIRQETLDNVRRLRHHACLGLWCGNNE
IESAWDHWGDFQKETPYLRADYVKQFEQILPHAVREADPDTFYWPSSPSSGGCFDEPDSE
DRGDTHYWDVWHGQKPFSDYRKYFFRFCSEFGFQSFPSYKTVESFTEEEDRNIFSKVMES
HQKNDAANGKMLYYLSENFRYPKDFKSLLYVTQILQAMAIKSGVEHWRRNRGRCMGTLYW
QVNDNWPVASWASIDYFGRWKALHYAAKHFYAQTAASICLEGETVGVYLENETMRGQQYE
AKLVLKTLDLEVLDCVEKKGTVKELSSELLIEKDYGELLKVRDKEEVFLEAVVTLSDGTV
LKETEIFVPYKYLKLKKPQVKATVTEEKDEYRIHLCADTFVPFLELDFADADAVFGDNYL
MLTEKKETVVTLKKEDITQGSFADAEDLKKRLVCRSVTDTY

Enzyme Prediction     

No EC number prediction in MGYG000000153_01982.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	721	2.3e-108	0.726063829787234

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	6.87e-106	1	704	10	687	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam17753	Ig_mannosidase	3.65e-16	737	819	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
PRK10340	ebgA	4.77e-14	176	374	195	394	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK09525	lacZ	2.40e-11	177	374	208	410	beta-galactosidase.
pfam00703	Glyco_hydro_2	3.81e-11	190	289	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCU02115.1	0.0	1	812	1	819
CBL20579.1	0.0	1	812	1	833
CBL08662.1	1.01e-302	6	800	9	836
VCV23589.1	1.43e-302	6	800	9	836
CBL13897.1	5.19e-302	6	800	16	843

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6BYE_A	1.42e-166	1	799	3	840	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]
6BYC_A	1.46e-166	1	799	3	840	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYI_A	1.05e-165	1	799	1	838	Crystalstructure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYI_B Crystal structure of the acid-base mutant (E477A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
6BYG_A	1.08e-165	1	799	3	840	Crystalstructure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306],6BYG_B Crystal structure of the nucleophile mutant (E575A) of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri [Xanthomonas citri pv. citri str. 306]
2VJX_A	4.86e-163	2	791	7	817	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q29444	6.85e-120	3	821	22	879	Beta-mannosidase OS=Bos taurus OX=9913 GN=MANBA PE=1 SV=1
Q95327	7.87e-117	3	821	22	879	Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=1 SV=1
Q4FZV0	3.28e-115	5	821	24	881	Beta-mannosidase OS=Rattus norvegicus OX=10116 GN=Manba PE=2 SV=1
A1CGA8	5.45e-115	6	793	9	829	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
Q8K2I4	2.34e-114	5	821	24	879	Beta-mannosidase OS=Mus musculus OX=10090 GN=Manba PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000049	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000153_01982.