CAZyme Information: MGYG000000159_02107

Basic Information

• Species: Sellimonas intestinalis
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Sellimonas; Sellimonas intestinalis
• CAZyme ID: MGYG000000159_02107
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
656	MGYG000000159_6|CGC2	76174.6	5.202

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000159	3206639	Isolate	China	Asia

• Gene Location: Start: 56161; End: 58131 Strand: +

Full Sequence      

MRGTKDFRPMDMKAYLIDDRFWKNKMEVVRTKMIPYQWKALNDLVEDAAPSHCVKNFRIA
AGMEKGDFQGCVFQDSDLAKWLEAVGYTLAWKKDRELEQMADEVIAVIEKAQQPDGYLDT
YYIINGLEKRWTNLRDNHELYCAGHMIEAGIAYYLGTGKHTLLDVVKRLADCIDRTFGPE
EGKIHAYPGHEIIEMALVRLYEATGEIRYLHLAKYFIDERGKEPLYFREEGEKYENPFYW
ENSLFQYQYYQAGKPVREQKTAEGHAVRAVYLYSGMTDVARIMEDDSLMEAVETLWDDVT
KRQMYITGAIGSSEYGESFTFDYDLPNDTVYGETCASIGLVFWAKRMLEKELSGQYADVM
ERALYNGCISGMSIDGTRFFYVNPLEADPEASRKDQRRKHVETSRWRWYDCACCPPNLAR
LIMSIGNYVCSVREDAIAFHLYVGGRVQTPFTDTQIRVESQMPWGGTTKLIIEKAKNSHY
LEKFTVALRIPGWCRKWKAEVNGEEVTDLPRNGYLYLERKWKDQDEIRLECSMQVERNYA
SPMVKYDIGKTAVSRGPFIYCLEEADNGKGLTRISLPSDSLFEMTEGNLETGELDLKAEG
MQMLAEGWEACLYRTKRPTKYEKKHLRFIPYYLWNNRGEGEMTVWVQETACDRAKE

Enzyme Prediction     

EC	3.2.1.185

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	17	561	3.9e-221	0.9942748091603053

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	0.0	1	648	3	587	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	1.60e-173	17	561	4	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
cd04791	LanC_SerThrkinase	0.004	149	214	89	169	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAB1245208.1	5.42e-275	15	648	15	643
AVK51332.1	1.62e-271	1	648	1	648
AIU03254.1	2.30e-271	8	648	8	648
ABR34523.1	2.30e-271	8	648	8	648
QUF76496.1	2.30e-271	8	648	8	648

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	8.27e-243	17	648	18	647	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	2.51e-234	17	648	18	647	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	3.38e-159	17	645	5	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	4.75e-159	17	645	5	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	1.34e-158	17	645	5	655	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.85e-158	17	645	5	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000159_02107.