CAZyme Information: MGYG000000169_01978

Basic Information

• Species: Eubacterium_G sp000432355
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Eubacterium_G; Eubacterium_G sp000432355
• CAZyme ID: MGYG000000169_01978
• CAZy Family: GH87
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
753		82876.8	9.9166

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000169	2836880	Isolate	China	Asia

• Gene Location: Start: 101518; End: 103779 Strand: +

Full Sequence      

MRKIVKQIIVMGMAVMLTIQPGYMGNDNKKVYGAESTKADSFYLPSANANGVIGATMPYT
RYDTEKASLAGGAVIAKSDSYSNSSIATQASNQSYARLPKSGSSVEWTMETSGDGVTMRF
TMPDSSDGKGKTGSLDVYVNGKLAEKVNLTSYYMWQYFAAGKGHPSDTNNGGYPLFAFDE
VHFKLNKSLKKGDKIKIQNSGAGNMEYGVDFIEVEQVQGEIKQPANSLSVVDYGAKPNDD
TDDATAINNCLYAAKNMKKDVYFPAGTYKMSKKMKILGENIKVTGAGMWYTNIQFTSDQQ
GGGGVTGKCKNVEISNMYLNSNLRSRYSESANYKCFADVFEGGSVIHDIWEEHFECGFWL
GDYDRDTNYSDGLKIVNSRIRNNLADGVNFCQGTSNAAVYNCSVRNNGDDGLAMWNNDYC
SKDEEKNVFAYNTVEFNWRAGGIAVYGGNGHKIYNNYICDTTMSCGIHLNTVFPGHKFNN
NKDGIKFDNNIIIKSGCSKDSWNGTMAAIDITGGVKNVTFSNTKIYEAQHDGIRVGQDCS
NIIFNNTKIYKSGADGNTSRKGAALYLDTAQNVIFNNIEIAKSAYSNAAGWPIYSHIYGT
VNTNNIKNYVNVSSKEYTIPSYPKAGTFSTGKPSNGGSENPTTKPTVKPTVKPIVKVNVT
KVKSASKKRASYKIKISLKKAKSVTGYQIQIATNKKFKKVLVSKKVKKYKFVLKSSKIKN
KKTLYLRARAYKVVSKKTYVSNWTKAKKVKVTK

Enzyme Prediction     

EC	3.2.1.59

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH87	53	587	3.4e-161	0.8375209380234506

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd14490	CBM6-CBM35-CBM36_like_1	1.20e-45	54	214	1	156	uncharacterized members of the carbohydrate binding module 6 (CBM6) and CBM35_like superfamily. Carbohydrate binding module family 6 (CBM6, family 6 CBM), also known as cellulose binding domain family VI (CBD VI), and related CBMs (CBM35 and CBM36). These are non-catalytic carbohydrate binding domains found in a range of enzymes that display activities against a diverse range of carbohydrate targets, including mannan, xylan, beta-glucans, cellulose, agarose, and arabinans. These domains facilitate the strong binding of the appended catalytic modules to their dedicated, insoluble substrates. Many of these CBMs are associated with glycoside hydrolase (GH) domains. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold. CBM36s are calcium-dependent xylan binding domains. CBM35s display conserved specificity through extensive sequence similarity, but divergent function through their appended catalytic modules.
pfam12708	Pectate_lyase_3	2.99e-08	229	419	3	176	Pectate lyase superfamily protein. This family of proteins possesses a beta helical structure like Pectate lyase. This family is most closely related to glycosyl hydrolase family 28.
pfam13229	Beta_helix	1.81e-07	368	546	6	155	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
pfam13229	Beta_helix	4.24e-07	390	577	4	156	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
pfam13229	Beta_helix	0.002	441	608	1	131	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABX41685.1	3.64e-203	39	636	185	783
ANR73273.1	2.89e-196	34	631	19	631
QUB45559.1	2.97e-196	34	631	20	632
QUB63242.1	1.32e-194	34	631	20	632
ASE16638.2	7.16e-194	34	631	19	631

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5ZRU_A	1.29e-157	54	536	13	505	ChainA, Alpha-1,3-glucanase [Niallia circulans],5ZRU_C Chain C, Alpha-1,3-glucanase [Niallia circulans]
7C7D_A	1.03e-42	54	511	54	476	Crystalstructure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3 [Streptomyces thermodiastaticus],7C7D_B Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3 [Streptomyces thermodiastaticus]
6K0M_A	6.50e-28	54	476	14	422	Catalyticdomain of GH87 alpha-1,3-glucanase from Paenibacillus glycanilyticus FH11 [Paenibacillus glycanilyticus],6K0N_A Catalytic domain of GH87 alpha-1,3-glucanase in complex with nigerose [Paenibacillus glycanilyticus]
6K0P_A	6.45e-27	54	476	14	422	Catalyticdomain of GH87 alpha-1,3-glucanase D1045A in complex with nigerose [Paenibacillus glycanilyticus]
6K0S_A	6.45e-27	54	476	14	422	Catalyticdomain of GH87 alpha-1,3-glucanase D1069A in complex with nigerose [Paenibacillus glycanilyticus],6K0V_A Catalytic domain of GH87 alpha-1,3-glucanase D1069A in complex with tetrasaccharides [Paenibacillus glycanilyticus],6K0V_B Catalytic domain of GH87 alpha-1,3-glucanase D1069A in complex with tetrasaccharides [Paenibacillus glycanilyticus],6K0V_C Catalytic domain of GH87 alpha-1,3-glucanase D1069A in complex with tetrasaccharides [Paenibacillus glycanilyticus],6K0V_D Catalytic domain of GH87 alpha-1,3-glucanase D1069A in complex with tetrasaccharides [Paenibacillus glycanilyticus]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.041665	0.694413	0.263119	0.000293	0.000241	0.000236

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000169_01978.