CAZyme Information: MGYG000000171_00328

Basic Information

• Species: Blautia_A sp900120195
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Blautia_A; Blautia_A sp900120195
• CAZyme ID: MGYG000000171_00328
• CAZy Family: GH25
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
547		60796.72	9.8246

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000171	3528861	Isolate	China	Asia

• Gene Location: Start: 325855; End: 327498 Strand: +

Full Sequence      

MAEIKGIDVSSFQGKPDWTKVKNSGIKFAILRIHQKSGIDTSFEHNYKGCKSNGILIGGY
KYSYALTPAQAIDEAEDVLSVLGGRGLDFPVFYDLEWSQQRSFGKQAIENIAVAFLTRIK
KAGYKVGIYCNLDWYNNVLSDALKQYDCWIARYPANDNGSVQERLRPNVGVGWQYSSKGK
VSGISGNVDMDVFYTDYRTEQKGEVTVAKTKLQKFTELGDYYANNGSNKPYLEKRTNAYL
DDFQKNAGYNNYTKFARDVDNWGQPGCQGQPWCAEYQFWKLVKVLGITKALKIMGGGFYN
CQSITRHAKANGTWHSTPKDGALIIFRNGSHVGSVRSFDFSKVHTNEGNTSSAAGVVANG
GAVRNKSYAISDPAIDGYVWIDWGEEKTVVETWKAIGTATSTVDDLYVRETPNGYVLGQI
NKGNRVEINGEKSGMWTKVKVAGIGIGWAATKYLQVDRAKSKPTTITNKQNKTQRLFVGK
VTATSTDVRTWAGGNYPSIKKWPKLVRGNLVDVMNFTQKATNGASWHYVRIAGKYYGFVA
AKDICKV

Enzyme Prediction     

No EC number prediction in MGYG000000171_00328.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH25	7	184	6.7e-43	0.9943502824858758

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06414	GH25_LytC-like	1.20e-68	4	194	1	191	The LytC lysozyme of Streptococcus pneumoniae is a bacterial cell wall hydrolase that cleaves the beta1-4-glycosydic bond located between the N-acetylmuramoyl-N-glucosaminyl residues of the cell wall polysaccharide chains. LytC is composed of a C-terminal glycosyl hydrolase family 25 (GH25) domain and an N-terminal choline-binding module (CBM) consisting of eleven homologous repeats that specifically recognizes the choline residues of pneumococcal lipoteichoic and teichoic acids. This domain arrangement is the reverse of the major pneumococcal autolysin, LytA, and the CPL-1-like lytic enzymes of the pneumococcal bacteriophages, in which the CBM (consisting of six repeats) is at the C-terminus. This model represents the C-terminal catalytic domain of the LytC-like enzymes.
cd00599	GH25_muramidase	2.27e-35	5	193	1	186	Endo-N-acetylmuramidases (muramidases) are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues. This family of muramidases contains a glycosyl hydrolase family 25 (GH25) catalytic domain and is found in bacteria, fungi, slime molds, round worms, protozoans and bacteriophages. The bacteriophage members are referred to as endolysins which are involved in lysing the host cell at the end of the replication cycle to allow release of mature phage particles. Endolysins are typically modular enzymes consisting of a catalytically active domain that hydrolyzes the peptidoglycan cell wall and a cell wall-binding domain that anchors the protein to the cell wall. Endolysins generally have narrow substrate specificities with either intra-species or intra-genus bacteriolytic activity.
cd06525	GH25_Lyc-like	1.86e-34	5	193	1	184	Lyc muramidase is an autolytic lysozyme (autolysin) from Clostridium acetobutylicum encoded by the lyc gene. Lyc has a glycosyl hydrolase family 25 (GH25) catalytic domain. Endo-N-acetylmuramidases are lysozymes (also referred to as peptidoglycan hydrolases) that degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.
cd06524	GH25_YegX-like	1.49e-29	5	194	1	193	YegX is an uncharacterized bacterial protein with a glycosyl hydrolase family 25 (GH25) catalytic domain that is similar in sequence to the CH-type (Chalaropsis-type) lysozymes of the GH25 family of endolysins.
pfam01183	Glyco_hydro_25	3.49e-29	7	184	1	180	Glycosyl hydrolases family 25.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCN31970.1	7.35e-42	2	202	395	590
QKN24292.1	1.80e-41	5	197	4	191
ARP50082.1	1.80e-41	5	197	4	191
ADU27377.1	6.97e-41	4	199	3	193
AVQ96437.1	6.97e-41	4	199	3	193

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4KRU_A	6.13e-20	4	193	20	207	X-raystructure of catalytic domain of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101]
4KRT_A	4.49e-19	4	193	20	207	X-raystructure of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101],4KRT_B X-ray structure of endolysin from clostridium perfringens phage phiSM101 [Clostridium phage phiSM101]
2WAG_A	4.90e-16	3	194	15	207	TheStructure of a family 25 Glycosyl hydrolase from Bacillus anthracis. [Bacillus anthracis str. Ames]
5A6S_A	4.23e-14	8	204	26	212	Crystalstructure of the CTP1L endolysin reveals how its activity is regulated by a secondary translation product [Clostridium phage phiCTP1]
4JZ5_A	1.27e-10	7	203	26	222	High-resolutionstructure of catalytic domain of endolysin ply40 from bacteriophage P40 of Listeria monocytogenes [Listeria phage P40]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P34020	3.10e-18	4	193	1	180	Autolytic lysozyme OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=lyc PE=1 SV=1
P26836	1.28e-17	4	193	9	196	Probable autolytic lysozyme OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=lyc PE=3 SV=2
P25310	2.13e-09	4	193	82	279	Lysozyme M1 OS=Streptomyces globisporus OX=1908 GN=acm PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000056	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000171_00328.