CAZyme Information: MGYG000000174_02175

Basic Information

• Species: Parabacteroides faecis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae; Parabacteroides; Parabacteroides faecis
• CAZyme ID: MGYG000000174_02175
• CAZy Family: GH97
• CAZyme Description: Retaining alpha-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
663	MGYG000000174_5|CGC3	75675.32	5.5068

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000174	6515019	Isolate	China	Asia

• Gene Location: Start: 130430; End: 132421 Strand: -

Full Sequence      

MKVQVLLTFLLLGSMPLLAQKSYQLQSPDRKLQTVVTVGDNVEFSLNHDGTEVLAPSAIS
MTLQTGEVLGGNSPKVSKVTKGSVDKSIPSPFYKKSEVSDIYNEMTIAFRGDYSIVFRLY
NDGMAYRFVTKKKGDMIVKDEEASYNFSTDHKTFAAYVNSTKPTFEEQFSNSFEQPYYNE
PITKLNDKRLMILPFLVDLGNGKKVCITEADLESYPGMFLNNTTDKPSLKSVHAPYPKVK
QQGGHNQLQMLVKERENYIAKTKGNRSFPWRVFVVSDNDKQLADCDMVYRLAAPSRVKDI
SWIKPGKVAWDWWNDWNISGVDFRAGINNETYKYYIDFAADHGIEYVILDEGWAVNLKAD
LLQVIPEINLEEIVSYAKSKNVDIILWAGYYAFDRDMENVVKRYADMGVKGFKVDFMDRD
DQEIIDFLYRAAETCARYNMLVDFHGICKPTGLQRTYPNVINYEGVNGLEQLKWSPESYD
MVTYDVTFPFIRMVAGPVDYTQGAMRNAIRQNYRPVYSEPMSQGTRCRQLATYVIFESPL
NMLCDNPTNYMREKVCTEFIAAIPTVWEETQVLDAKVSEYIAMARKHGDNWYVGALTNWE
PRELELDLSFLGDGDYKLELFKDGINADRAASDYKKEIIPVPADRKLKIKMAPGGGYAAK
ICK

Enzyme Prediction     

EC	3.2.1.22

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	15	661	8.5e-212	0.9873217115689382

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	1.91e-140	299	564	1	278	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	4.11e-71	27	294	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	1.62e-45	567	661	1	96	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT47767.1	0.0	1	663	1	662
QUR50186.1	0.0	1	663	1	662
QIX67464.1	0.0	1	663	1	662
BBK91654.1	0.0	12	663	1	650
QJE27063.1	0.0	1	663	1	663

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3A24_A	4.78e-291	27	661	6	640	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
5E1Q_A	9.16e-290	27	661	20	654	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
5HQC_A	1.51e-66	26	660	4	657	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQ4_A	2.09e-66	26	660	4	657	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQB_A	5.55e-66	26	660	4	657	AGlycoside Hydrolase Family 97 enzyme (E480Q) in complex with Panose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8A6L0	6.30e-294	7	661	4	661	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
D7CFN7	3.22e-49	28	662	46	621	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1
G8JZS4	1.09e-48	94	661	116	723	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000273	0.999005	0.000214	0.000176	0.000158	0.000146

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000174_02175.