CAZyme Information: MGYG000000175_03655

Basic Information

• Species: Muricomes sp000509105
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Muricomes; Muricomes sp000509105
• CAZyme ID: MGYG000000175_03655
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
634	MGYG000000175_25|CGC1	72668.47	5.102

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000175	5055107	Isolate	China	Asia

• Gene Location: Start: 43409; End: 45313 Strand: +

Full Sequence      

MKQTKMEIRDIFWSGQIEKMCKCIIPYQYEVLSDAVPGIPKSHAIENFRIAAKLAEGEYA
GMLFQDSDVAKWIEGAAYSLRIKENPKLESQIDELIEIIGRAQQSDGYLNTYYTCAGKGK
RLTNIAHGHEMYCAGHLAEAAAAYAEATGKERLLQIVERYMDWFMERIGPEEGKLHIYPG
HPELELALYRLYQYTQKEKYLAFMKYLLLERGKQPSFLLNDSGFGEQFKDKWFGLEYHQA
QKPVQEQSEAVGHAVRAMYLYSGLADLAYETGDEKTIRALDALWQDVTKRKMYITGAVGA
DEHGEAFSVPYDLPNDRAYAETCASIGLVFWARRMLRLKLDSQYSDIMELALYNGVLSGM
SDNGKEYFYVNPLMLNPREADQRYDLRHVETSRVTWFGCACCPPNAARTITSLDKYVYDF
NQEENQLAIHLYIGGKIYLEDGGLWEITGDYLGDGRLEIKYFGPKKEMSVRFRKPGWSKS
IGVLDNGEEIQPEEVTGYMEINRAWCDGDVIQLQFDIQSRFVYANPKVSADSGRAAILRG
PIVYCLEEADNGEDLNALIAESICGTKEMGQEREGREEFYPPVYIRGYREYQNPDNHELY
SFEQPARKKVTMKAVPYARWGNRGLGEMLVWVRR

Enzyme Prediction     

EC	3.2.1.185

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	7	545	1.2e-193	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	6.48e-174	24	634	30	587	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	1.26e-143	46	545	30	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG4225	YesR	0.001	148	202	52	102	Rhamnogalacturonyl hydrolase YesR [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGH34297.1	1.26e-215	6	633	13	639
QNF29330.1	1.14e-196	3	632	8	641
AGT32221.1	1.61e-196	4	632	6	637
AGB41651.1	3.93e-195	1	633	1	644
AOL36265.1	6.24e-195	5	632	7	637

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	6.79e-195	4	632	14	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.69e-187	4	632	14	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	4.03e-142	12	634	9	658	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	5.58e-142	12	634	9	658	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	1.57e-141	12	634	9	658	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	2.21e-141	12	634	9	658	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000175_03655.