CAZyme Information: MGYG000000195_03163

Basic Information

• Species: Faecalibacterium prausnitzii_E
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Faecalibacterium; Faecalibacterium prausnitzii_E
• CAZyme ID: MGYG000000195_03163
• CAZy Family: GH3
• CAZyme Description: Thermostable beta-glucosidase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
805	MGYG000000195_16|CGC2	86361.55	5.6207

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000195	3389403	Isolate	China	Asia

• Gene Location: Start: 52846; End: 55263 Strand: -

Full Sequence      

MKHADIIQTLDLEQKCALLSGGTVFDTRALPGKGIPSITLSDGPNGVRKQAGAADHLGLN
PSVPATCFPTSATVANSWDPALGEAVGEAMGEEAAAQEVSVLLGPGLNIKRSPLCGRNFE
YFSEDPYLAGKMAAGYVRGIQKNGIAACPKHFAVNSQELRRMASDSIVDERTLRELYLTG
FEIVVKEAKPKTIMSSYNLVNGTYANENAHLLQDILRRDWGFDGAVVTDWGGSNDHALGV
KNGSTLEMPFPGDDSVRELMKAVESGRISEHDVDARLDELLELVLDTKAAVEKAPRTFDA
AAHHALARRAAAQSIVLLRNEGALLPLKKGEKIAVLGDFARTPRYQGAGSSAVNSIQVDS
FLDCLTESGLANVGYAQGFDRQGKADEALKKEAVALAQNANVVLLCMGLDEIKESEGLDR
MDMKLAQNQLDLLAAVAAVNPNVVVLLSAGSSLETPWLKDCKALVYGCLGGQAGAGALVD
VLTGTVCPGGKLAETWANAYSDSPVKDHFAGEGRTVQYREGLYVGYRYFETAGRPVAFPF
GYGLSYTTFAYKDLKAAPEGVTLTVTNTGKCAGAEIVQLYVAKPDAKVFRPAQELKGFAK
VWLEAGESKTVSIPLDDKAYRYWNVATDRWEVEGGSYQLRVGASSADIRLTADVVVLGSG
APDPYQSVDLPHYRTGEITSVPDAEFAALLGRPIPEDKIRIDRNMTLGELGHGRSPLGWL
VAAVLGLLLKRSIQRGKPDLNILFQYNMPLRALAKMTNGAISMGMVDGIVMEAQGFWIIG
LLRVIVEAVKNIAQNGAMEKRLKNS

Enzyme Prediction     

EC	3.2.1.21	3.2.1.-	3.2.1.45

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	33	237	5.2e-65	0.9120370370370371

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	1.04e-72	66	649	118	758	beta-glucosidase BglX.
COG1472	BglX	1.72e-54	31	340	56	364	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
PLN03080	PLN03080	4.22e-49	6	616	56	743	Probable beta-xylosidase; Provisional
pfam01915	Glyco_hydro_3_C	3.64e-42	315	526	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
pfam00933	Glyco_hydro_3	1.64e-32	65	276	85	309	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CBL01314.1	0.0	1	805	1	805
AXB27517.1	0.0	1	805	1	805
AXA81990.1	0.0	1	805	1	807
CBK98877.1	0.0	1	805	1	807
QOY61156.1	0.0	1	802	1	802

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MS2_A	2.10e-195	6	741	8	752	ChainA, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2],7MS2_B Chain B, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2]
5WAB_A	5.90e-185	7	664	9	671	CrystalStructure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_B Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_C Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_D Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703]
2X40_A	2.30e-121	2	650	3	707	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with glycerol [Thermotoga neapolitana DSM 4359],2X41_A Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with glucose [Thermotoga neapolitana DSM 4359]
2X42_A	3.46e-120	2	650	3	707	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with alpha-D-glucose [Thermotoga neapolitana DSM 4359]
3AC0_A	4.51e-117	6	648	9	828	Crystalstructure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_B Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_C Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_D Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P14002	1.63e-194	6	656	8	668	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2
E7CY69	4.39e-189	7	711	9	741	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium longum OX=216816 GN=apy PE=1 SV=1
F6C6C1	4.39e-189	7	664	9	680	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium breve (strain ACS-071-V-Sch8b) OX=866777 GN=HMPREF9228_1477 PE=1 SV=1
A7LXS8	1.65e-126	5	651	48	740	Beta-glucosidase BoGH3A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02644 PE=1 SV=1
Q5BFG8	2.50e-122	6	648	14	830	Beta-glucosidase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bglB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.994899	0.005053	0.000055	0.000010	0.000005	0.000009

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000195_03163.