CAZyme Information: MGYG000000196_02066

Basic Information

• Species: Bacteroides thetaiotaomicron
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides thetaiotaomicron
• CAZyme ID: MGYG000000196_02066
• CAZy Family: GH97
• CAZyme Description: Glucan 1,4-alpha-glucosidase SusB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
671	MGYG000000196_8|CGC3	76078.45	5.473

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000196	6397474	Isolate	China	Asia

• Gene Location: Start: 114051; End: 116066 Strand: +

Full Sequence      

MKVKHLVGAFLCVLGCYACSSPKTEVQSPDGHIKMTLTVDENGKPFYNVSVDDSLLIENS
AMGFTAANGIILAEGFQVKNTTFSSEDETWTQPWGENKKNRNHYNEMAVSLTNEDQVQLT
LRFRVFDDGIGFRYEYTVPTVDSLLITDELTTFRFHRDGTSWSIPASAETYELLYQQQPI
SEVETANTPFTFKTADGVYGSIHEAALYDFPEMTLKQIGHYTLKAELAPWPDGIKARKGN
YFTTSWRTIQIAPEAVGLINSSLILNLNDPCVLETTDWIRPLKYVGVWWGMHLGVETWKM
DERHGATTANAKKYIDFAAANHIEAVLFEGWNEGWESWGGMQSFDFTKPYADFDIDEIVR
YAKEKGVEIMGHHETGGNIPNYERQMDHAMQWYTDHGIHLLKTGYAGAFPDGYLHHSQYG
VNHYQRVVETAARHQMTLDAHEPIKDTGIRRTWPNMMTREGARGMEWNAWSEGNPPSHHV
MLPFTRLLSGPMDYTPGTFDILFLKTKDSPRRQKWNDQDKGNSRVNTTLAKQLANWVILY
SPLQMASDMIEHYEGHPAFQFFRDFDPDCDESKALAGEPGEFIAVVRKAKGNYFLGAATN
ESPRTLEVKLDFLEPGKQYKAVIYADGEKADWKTNPTDYKITEQIVTSENTLSIRMAPGG
GQAVSFIKSNR

Enzyme Prediction     

No EC number prediction in MGYG000000196_02066.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	13	666	1.1e-235	0.9920760697305864

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	5.01e-149	276	565	2	277	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	3.03e-87	28	270	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	3.22e-44	569	667	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT71305.1	0.0	1	671	1	671
QMW87725.1	0.0	1	671	1	671
AAO75790.1	0.0	1	671	1	671
BCA50408.1	0.0	1	671	1	671
ALJ41824.1	0.0	1	671	1	671

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HQ4_A	2.23e-192	26	666	3	658	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQC_A	2.23e-192	26	666	3	658	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQB_A	6.31e-192	26	666	3	658	AGlycoside Hydrolase Family 97 enzyme (E480Q) in complex with Panose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
3WFA_A	9.97e-166	25	666	4	703	Catalyticrole of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron],3WFA_B Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron]
2D73_A	1.02e-165	20	666	19	723	CrystalStructure Analysis of SusB [Bacteroides thetaiotaomicron VPI-5482],2D73_B Crystal Structure Analysis of SusB [Bacteroides thetaiotaomicron VPI-5482],2ZQ0_A Crystal structure of SusB complexed with acarbose [Bacteroides thetaiotaomicron],2ZQ0_B Crystal structure of SusB complexed with acarbose [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
G8JZS4	4.96e-166	2	666	5	723	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
Q8A6L0	3.40e-59	3	666	1	661	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
D7CFN7	8.55e-31	13	610	37	574	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000001	0.000045	0.999982	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000196_02066.