CAZyme Information: MGYG000000196_03066

Basic Information

• Species: Bacteroides thetaiotaomicron
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides thetaiotaomicron
• CAZyme ID: MGYG000000196_03066
• CAZy Family: GH31
• CAZyme Description: Oligosaccharide 4-alpha-D-glucosyltransferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
717	MGYG000000196_13|CGC3	79924.22	7.4966

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000196	6397474	Isolate	China	Asia

• Gene Location: Start: 94520; End: 96673 Strand: -

Full Sequence      

MKYTCIKTVAIYLLATLPLLANASTHKVKITHSVMVGDGIAKFIPEGFDAQKIPSFAIEK
EPREQGALPADWILVPEFSLTNGKANASLTVPEGTSIYGGGEVTGSLLRNGKTIKLWNTD
SGAYGVDKGTRLYQSHPWMMGVRKDGTAFGILFDTTWKAELSSTDEKIELKSEGIPFRVF
IIDRESPQAVVRGLSELTGTMPMIPRWALGYQQCRFSYSPDSRVIEIADTFRLKRIPCDV
IWMDIDYMDGYRIFTFNPKSFPNPKAVNRDLHIRGFHSAWMIDPGAKVDPNYFVYKSGTE
NDVWVKTADGKNFHGDAWPGAAAFPDFTSPKVNKWWRNLYKDFLAQGVDGVWNDVNEPQI
NDTPNKTMPEDNLHRGGGKLPAGTHLQYHNVYGFLMVKASREGILDVRPEKRPFILTRSN
FLGGQRYAATWTGDNGSCWDHLKMSVPMSLTLGLSGQPFSGADIGGFLFNADADLFGNWI
GFGAFYPFARGHACAGTNNKEPWVFGQKVEDASRIALERRYMLLPYFYTLLHEASTNGMP
IMRPVFFSDPKDLSLRAEEEAFLVGDNLLIIPAFANQPALPKGIWKELSLVEGDQNDKYQ
AKMKIRGGAIIPTGKIIQNTTENSLDPLTLLVCLDEQGKASGNMYWDAGDGWSYKKGDYS
LLQFVAERNGDKVTVKLTKKTGKYNTENKDMAVIKIITDQGIRQASGNLVEGIEIRL

Enzyme Prediction     

EC	3.2.1.20

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	180	602	1.7e-135	0.9625292740046838

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06604	GH31_glucosidase_II_MalA	0.0	199	538	1	339	Alpha-glucosidase II-like. Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This subgroup also includes the MalA alpha-glucosidase from Sulfolobus solfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source.
pfam01055	Glyco_hydro_31	0.0	180	611	1	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
PLN02763	PLN02763	0.0	55	693	34	677	hydrolase, hydrolyzing O-glycosyl compounds
cd06603	GH31_GANC_GANAB_alpha	2.19e-166	199	650	1	466	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
COG1501	YicI	8.90e-157	69	707	127	767	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT41209.1	0.0	1	717	1	717
AAO78405.1	0.0	35	717	1	683
QMW89162.1	0.0	35	717	1	683
QUT73399.1	0.0	1	717	1	717
QQA08573.1	0.0	1	717	1	717

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5DJW_A	0.0	34	717	1	684	Crystalstructure of Family 31 alpha-glucosidase (BT_3299) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5DJW_B Crystal structure of Family 31 alpha-glucosidase (BT_3299) from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
5F0E_A	2.42e-115	49	663	139	776	Murineendoplasmic reticulum alpha-glucosidase II [Mus musculus],5H9O_A Complex of Murine endoplasmic reticulum alpha-glucosidase II with D-Glucose [Mus musculus],5H9O_C Complex of Murine endoplasmic reticulum alpha-glucosidase II with D-Glucose [Mus musculus],5HJO_A Murine endoplasmic reticulum alpha-glucosidase II with bound substrate analogue [Mus musculus],5HJO_C Murine endoplasmic reticulum alpha-glucosidase II with bound substrate analogue [Mus musculus],5HJR_A Murine endoplasmic reticulum alpha-glucosidase II with bound covalent intermediate [Mus musculus],5HJR_C Murine endoplasmic reticulum alpha-glucosidase II with bound covalent intermediate [Mus musculus]
7KBJ_A	4.62e-112	168	663	6	516	ChainA, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7KBJ_C Chain C, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7KBR_A Chain A, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7KBR_C Chain C, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7L9E_A Chain A, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus],7L9E_C Chain C, Neutral alpha-glucosidase AB Trypsin-cleaved Fragment #3 [Mus musculus]
5IEF_A	1.01e-110	111	663	242	831	Murineendoplasmic reticulum alpha-glucosidase II with N-butyl-1-deoxynojirimycin [Mus musculus]
5IEG_A	1.03e-110	111	663	242	831	Murineendoplasmic reticulum alpha-glucosidase II with N-9'-methoxynonyl-1-deoxynojirimycin [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9F234	1.13e-149	93	708	145	769	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1
Q9FN05	3.42e-117	91	665	229	834	Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1
B9F676	1.08e-112	115	669	256	836	Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1
Q8BHN3	1.05e-109	111	663	274	863	Neutral alpha-glucosidase AB OS=Mus musculus OX=10090 GN=Ganab PE=1 SV=1
P79403	7.75e-109	147	663	333	863	Neutral alpha-glucosidase AB OS=Sus scrofa OX=9823 GN=GANAB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000258	0.998996	0.000233	0.000161	0.000167	0.000150

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000196_03066.