CAZyme Information: MGYG000000196_03359

Basic Information

• Species: Bacteroides thetaiotaomicron
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides thetaiotaomicron
• CAZyme ID: MGYG000000196_03359
• CAZy Family: GH2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
734	MGYG000000196_15|CGC4	83721.38	6.4641

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000196	6397474	Isolate	China	Asia

• Gene Location: Start: 133043; End: 135247 Strand: +

Full Sequence      

MNKKLLIAVFVSAMAHTFACAASPKENRMKTVWADKVTSENVWQSYPRPQLQRSQWMNLN
GLWQYAVTTQDTPKKEVKFEGQILVPFAIESSLSGVQRSFLPTEKLWYQRNFTLDDAWKG
KTVILHFGAVDYECQVWVNNKLAGIHKGGNNPFSFDVTKLLRKGGSQLVEVAVIDPTDTE
SISRGKQQLEPKGIWYSPVSGIWQTVWLEAVNSTHILQVLPTADIHKKTVSLDISVAKAK
GGEKVKVELLDEGKVVGTTEQKLASKIEMEVPEAVLWSPSSPKLYHLNIELLSQGQVIDQ
VKSYFAMREVSIRKDECGYQRICLNGDPIFQYGTLDQGWWPDGLLTPPSEEAMLWDMVQL
KNMGFNTIRKHIKVEPEQYYYYADSLGLMMWQDMVSGFSTERKDEEHIKPNAQTDWNAPA
SHTNQWQQEMFEMIDRLRFYSCITTWVIFNEGWGQHNTVEIVEKVMEYDKSRIIDGVTGW
TDRGVGHMYDVHNYPSASMILPACNGNRISVLGEFGGYGWAIKEHLWNPGMRNWGYKNID
GAMALMDNYGRLVYDLETLIAQGLSAAIYTQTTDVEGEVNGLITYDRKVIKIPEGLLHIM
HDRLYHVNPAKVITLVPDAQNGSAVSRAVSLNGQESKMTSLPFDCPPRSEIVSETSFDVN
DEFSHLSLWVRATGDVKVWLNGVEVFSQEVKQTRQYNQYNISNYCRYLRKGKNELKIEVR
ETKKMSFDFGLRAY

Enzyme Prediction     

No EC number prediction in MGYG000000196_03359.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	39	498	6.6e-101	0.5319148936170213

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK10150	PRK10150	1.30e-31	48	451	3	417	beta-D-glucuronidase; Provisional
COG3250	LacZ	1.37e-31	59	529	15	484	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	6.07e-19	29	313	1	323	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	5.88e-17	59	208	4	165	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
pfam00703	Glyco_hydro_2	1.78e-11	214	308	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QQA08738.1	0.0	1	734	1	734
ALJ39628.1	0.0	1	734	1	734
AAO78198.1	0.0	1	734	1	734
QMW85514.1	0.0	1	734	1	734
QUT73209.1	0.0	1	734	1	734

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7SF2_A	1.54e-168	24	594	7	572	ChainA, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_B Chain B, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_C Chain C, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_D Chain D, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_E Chain E, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838],7SF2_F Chain F, Glycosyl hydrolase family 2, sugar binding domain protein [Bacteroides cellulosilyticus DSM 14838]
4YPJ_A	1.07e-19	90	472	56	437	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
4JKM_A	6.79e-19	55	451	14	415	CrystalStructure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],4JKM_B Crystal Structure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],6CXS_A Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13],6CXS_B Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13]
6ECA_A	9.58e-19	46	475	31	462	Lactobacillusrhamnosus Beta-glucuronidase [Lacticaseibacillus rhamnosus],6ECA_B Lactobacillus rhamnosus Beta-glucuronidase [Lacticaseibacillus rhamnosus]
3FN9_A	2.52e-18	107	488	63	437	Crystalstructure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_B Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_C Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343],3FN9_D Crystal structure of putative beta-galactosidase from bacteroides fragilis [Bacteroides fragilis NCTC 9343]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	7.65e-29	107	477	59	415	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KPJ7	3.12e-18	107	394	106	389	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P23989	1.90e-17	108	472	124	479	Beta-galactosidase OS=Streptococcus thermophilus OX=1308 GN=lacZ PE=3 SV=1
P06864	4.92e-13	78	313	81	323	Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4
Q02603	7.46e-12	108	474	132	489	Beta-galactosidase large subunit OS=Leuconostoc lactis OX=1246 GN=lacL PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000624	0.340243	0.658667	0.000174	0.000150	0.000137

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000196_03359.