CAZyme Information: MGYG000000202_02087

Basic Information

• Species: AF33-28 sp003477885
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; AF33-28; AF33-28 sp003477885
• CAZyme ID: MGYG000000202_02087
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1327	MGYG000000202_6|CGC6	152289.64	5.2055

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000202	5162288	Isolate	China	Asia

• Gene Location: Start: 197362; End: 201345 Strand: +

Full Sequence      

MMDTVKSSRRDCLCLNGEWDFMPYLDREAADELPDPIVYEEEKVCVPSSWRGFDDKFHIP
EYDFAPMDVFAYPKHWAKAEAGLLHRTFRVPEEWKGKKIVLRCDGVFQKSVIWLDRIKAG
TFSEAFLPICLDITPLVRYGEEQELYVQCGPFDTVEIPSGARKTTGLGGNWFGRFARGIW
QDIYLSALPFARVEDVEITTSVRNGIIEGRILVENEADTPLCGTLAIRVSEWKSGRVMWE
AEQSIVLNPLSEKEIYYKRPWKDAVYWDTENPFLYCWETKLTAADGRYDVHTVRFGFREF
WTEGDLFYLNGIPIKLRGDSWHFQGAVQQTKAYAENWFSICRESGVNSVRLHAEPHPAYY
LDAADEMGMLIVDETAIYGSGKAMDASCPVFLDNCRRHVQELVKRDRNHPSVVIWSLENE
MRWVEGRDVYKKHIPGLMALMHAADHAGRPISLDGDNRLISGEHTEIASLHYNIDGTISQ
WDHSTPLTIGEHGGHWYLCPQNASMYEGMGVYTDSEVCCHAVALKEKLFLEYARRMDVTG
ISSFNIAHYFAVSMPDRDIPLPPVDREGRWGVYPKLIRKYSMTIGNGILEKYGYPRYKPN
PAYPLIAEAMRPVTAILREYDQAFYEGRAFIRTFDIYHDVQSRVSLRLEVECVQEKRSVC
RTAFCLDMDPAGHEVRTVEVLPCLCGGDEPETMILSWRLFAGEKMVEKKEKSYKIFPAFR
GTDKRMSTAFLYVGINGSEYRLLERQFGNVTLWEEQEQMPDGALIIIGSHQTDRFEWLEE
RMDGWIRDGHPILCLAQHAFAYGDLELECRMYLGAYEGCRSHPLLMGMDRNAFFYWNGEI
SEAGPVPDFLWAYRKAEAGNIRYPLECGCGDFGDGGDLWSALVEYRFDESSMLLCQLELE
QYYEKVPYAAQLLERMAAYLLMRQEEIRQKERKVLYIGCEEGSSLPGLLDETKAAWKMLF
EDGIQTEKLPERGCLLADAAWIKGRETWLSQWAAGGGHLLIQGLRPGMEESIALLAGADI
EILPETVWQFETLDGDFLRDISIADCYGMDLVHMCPRRVENRKLCEYTVQSGEGSMLARR
TAKTVWEDTFVKKHSAEYCRRSLVEINRRKEETRGGCAWQFDLGRGRILISQIAADGGYG
KSVRLYTGILDREHISFARKSFRTEAAFRAREEMRQAVNEWMVLPCEPWQDLEAMRRYYT
DPEYSLNNLGEGLYGWMKKAEKCRSDGGWVLVDQWNGCPLFMTCFVCTEVKTEVRLCLNA
SRSARVYCNGEPAGDTVWLKPGSNVFCVEAKPGKEPFRMRLRLTDGGGHALAGRLWYRLT
VDEIDPK

Enzyme Prediction     

No EC number prediction in MGYG000000202_02087.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	6	511	3.6e-76	0.550531914893617

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.72e-39	3	420	6	406	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	5.98e-31	15	420	44	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
PRK10150	PRK10150	1.15e-30	1	420	1	417	beta-D-glucuronidase; Provisional
PRK09525	lacZ	1.79e-16	15	428	55	480	beta-galactosidase.
pfam02837	Glyco_hydro_2_N	7.62e-12	15	189	4	169	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CQR58310.1	0.0	10	1327	2	1343
AIQ71089.1	0.0	10	1327	2	1343
AIQ60999.1	0.0	10	1327	2	1389
QUL54273.1	0.0	10	1327	3	1418
AIQ32428.1	0.0	10	1327	2	1408

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5T98_A	4.37e-25	82	421	86	418	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
3BGA_A	5.63e-25	15	420	51	464	Crystalstructure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482],3BGA_B Crystal structure of beta-galactosidase from Bacteroides thetaiotaomicron VPI-5482 [Bacteroides thetaiotaomicron VPI-5482]
3DEC_A	7.33e-25	15	420	47	460	ChainA, Beta-galactosidase [Bacteroides thetaiotaomicron VPI-5482]
6S6Z_A	6.97e-24	13	420	39	440	Structureof beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_B Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_C Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_D Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_E Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_F Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_G Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8],6S6Z_H Structure of beta-Galactosidase from Thermotoga maritima [Thermotoga maritima MSB8]
6SD0_A	6.97e-24	13	420	40	441	Structureof beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_B Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_C Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8],6SD0_D Structure of beta-galactosidase from Thermotoga maritima. [Thermotoga maritima MSB8]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	5.45e-26	10	497	3	459	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
O52847	9.09e-26	3	420	46	481	Beta-galactosidase OS=Priestia megaterium (strain DSM 319 / IMG 1521) OX=592022 GN=bgaM PE=3 SV=1
A8GGN3	3.54e-25	15	428	56	479	Beta-galactosidase OS=Serratia proteamaculans (strain 568) OX=399741 GN=lacZ PE=3 SV=1
A8AKB8	3.64e-23	15	428	55	480	Beta-galactosidase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) OX=290338 GN=lacZ PE=3 SV=1
Q56307	3.82e-23	13	420	40	441	Beta-galactosidase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=lacZ PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000043	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000202_02087.