CAZyme Information: MGYG000000202_03852

Basic Information

• Species: AF33-28 sp003477885
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; AF33-28; AF33-28 sp003477885
• CAZyme ID: MGYG000000202_03852
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
896	MGYG000000202_17|CGC2	100903.68	5.0162

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000202	5162288	Isolate	China	Asia

• Gene Location: Start: 98132; End: 100822 Strand: +

Full Sequence      

MNRIELNGAWQCSFPDGQHLEVMVPGCFDVYRKEKDIADAVTLSRTFCAEIGEGKRALLY
FGGVSYYCDIYVNGRLAGSHEGMWDNFSTDVSGLLKNGENELTLTVWKPGYHKEDRFPLR
EVLSGFIPDVLCTFGGIWDDVYLLSADTYFIGRHCASASLDGAGKLTVDINCADQIPVKI
TGAVYGPDGRKAAAICAQREAGPGENRLEIPFELSEPDCWSPNRPQMYRYDLEITAGGQT
ERVENAFGCRSIQADDSRILLNGEPVYPRGILHWGYYSGLTPRPGAGVIRDEIAKCRQYG
FNMIKHCLYIPREEYLRQADEAGMLLWIELPLWLPDVSEELEPRILREFPRLLNQIKGHP
SVVMASLGCELDDKVGGAVLEEMYRLAKRELNCLVRDNSGSGECYGGLAVDYADFSDYHF
YGELQNMENLMENFTPVWKRNRPWIYGEFCDSDTMRDLAEVRGRMGKEELFWEKADPVEN
PISILKPDFYLGSHEERMKESGIRTDFSLIKELSFNHSMVHRKTTLEETRAFEEISGYNI
TSIRDVPIATSGIFDDFMEPKFDRKLFCSFNSDVMLIPAWDLGRTWIGADRVLPRERYNF
IGGSTLTRHILVSNYSGSALVDPVLSWSLETDGHAWAGGKETGRTVLSGTVRECAYLSFA
LPKAGRPSTAVLKVKLSSGAVECENRWPVFLYPELSCADVRVGLYDPCHIFDRTEPLFQS
MTRIADEQEIALDEIDAVLTSRLTPAVLRYVERGGKAVCVQRGTGPLPTVPVAFWREGMV
RYGSHPALGGIRYENWMDDLRFFGVGTDTSLDLTDPYYSHFTSAEPVILRYDCREWKKSA
YMAELRLGRGRILATTLRLEGGMGKQPLFITNNTLGRHLLMSAVLDLTGRNGGQAF

Enzyme Prediction     

No EC number prediction in MGYG000000202_03852.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	2	414	1.6e-62	0.4867021276595745

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	2.11e-30	2	388	26	421	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10150	PRK10150	2.42e-14	53	378	80	425	beta-D-glucuronidase; Provisional
PRK10340	ebgA	3.07e-14	45	390	115	466	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	3.91e-08	44	145	70	167	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.
pfam02836	Glyco_hydro_2_C	6.03e-07	256	449	5	210	Glycosyl hydrolases family 2, TIM barrel domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTH41860.1	5.04e-174	1	892	1	892
SDS96723.1	4.33e-146	22	880	27	917
ASW55283.1	1.21e-121	55	707	59	741
QNK55911.1	1.45e-74	59	860	409	1198
AIE85725.1	1.17e-68	16	888	18	751

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6D8K_A	2.45e-25	10	333	70	374	Bacteroidesmultiple species beta-glucuronidase [Bacteroides ovatus],6D8K_B Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_C Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus],6D8K_D Bacteroides multiple species beta-glucuronidase [Bacteroides ovatus]
7VQM_A	5.15e-22	21	373	75	412	ChainA, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_B Chain B, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_C Chain C, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_D Chain D, GH2 beta-galacturonate AqGalA [Aquimarina sp.]
6D89_A	7.71e-20	22	333	81	374	Bacteroidesuniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_B Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_C Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis],6D89_D Bacteroides uniformis beta-glucuronidase 1 with N-terminal loop deletion [Bacteroides uniformis]
6D1N_A	7.92e-20	22	333	89	382	Apostructure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D1N_B Apo structure of Bacteroides uniformis Beta-glucuronidase 1 [Bacteroides uniformis],6D41_A Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D41_B Bacteriodes uniformis beta-glucuronidase 1 bound to D-glucaro-1,5-lactone [Bacteroides uniformis],6D6W_A Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_B Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_C Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D6W_D Bacteroides uniformis beta-glucuronidase 1 bound to glucuronate [Bacteroides uniformis],6D7F_A Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_B Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_C Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_D Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_E Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis],6D7F_F Bacteroides uniformis beta-glucuronidase 1 bound to thiophenyl-beta-D-glucuronide [Bacteroides uniformis]
4JKM_A	7.37e-19	4	469	16	528	CrystalStructure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],4JKM_B Crystal Structure of Clostridium perfringens beta-glucuronidase [Clostridium perfringens str. 13],6CXS_A Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13],6CXS_B Crystal Structure of Clostridium perfringens beta-glucuronidase bound with a novel, potent inhibitor 4-(8-(piperazin-1-yl)-1,2,3,4-tetrahydro-[1,2,3]triazino[4',5':4,5]thieno[2,3-c]isoquinolin-5-yl)morpholine [Clostridium perfringens str. 13]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P77989	7.88e-19	5	364	18	381	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KPJ7	1.16e-18	49	371	114	431	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
P06864	1.37e-11	53	388	124	464	Evolved beta-galactosidase subunit alpha OS=Escherichia coli (strain K12) OX=83333 GN=ebgA PE=1 SV=4
P24131	3.43e-10	53	388	136	471	Beta-galactosidase OS=Clostridium acetobutylicum OX=1488 GN=cbgA PE=2 SV=2
P05804	1.40e-09	10	469	35	524	Beta-glucuronidase OS=Escherichia coli (strain K12) OX=83333 GN=uidA PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000047	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000202_03852.