CAZyme Information: MGYG000000206_00113

Basic Information

• Species: Lachnoclostridium_B sp900066555
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Lachnoclostridium_B; Lachnoclostridium_B sp900066555
• CAZyme ID: MGYG000000206_00113
• CAZy Family: GH73
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
964	MGYG000000206_1|CGC1	107950.08	4.3872

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000206	2952936	Isolate	China	Asia

• Gene Location: Start: 128979; End: 131873 Strand: +

Full Sequence      

MKGLKRMVAVFLAAVMLCLMPVTDMSVMAEEISDTTVTENDSYQIEKSEIEKNVEADSGQ
DESQIEEVESQEVVSNGLLNYVGIDKPYLTSPDTQKIVVSYGDGTEAISEAKIVYQKADG
TIFEDTMSSRQEELFLFEHSFSGEDKGTYTLLRFVYVEDGVEQTIELQDIDVEAMFGVDE
EYPGYNSDSSDVISQEEIDTSVVSIDSDEVATAQGEIADAIGNTEEEEGIATYSSDEQTK
NGNVIVVLDPGHGGTDSGAVGNGLQEKDLTLKIAKYCKEELEKYSGVAVYMTREDDRYLG
NRVGDAASDLANRVNKAKEWGADLLVSIHINSGGGQGVEVYYPNSNYNSAIGQEGKDSAQ
KILDELLKLGLKNRGIKIRSANYDTYPDGSLEDWYGIIRRSKYEGFPGIIVEHAFIDNVN
DAEKLKDESFLKQCGVADATGIANHFGLSKGHWELDGSQWKYSNGDGTYKTSQWLLLNGV
WYYFDAKGIMQTGWTKVGDATYYMDAGGAMRTGWLYLDDTWYYLGTSGARVADCWQWINN
KCYYFDNDGCMAANTWIGDSYVDTSGAWVPGKQKVVSGWKVVSGRWKYMESNGEYVKSRW
LLVGSTWYHFDANGWMQTGWLLTGNTWYYLESSGAMIAGRWQWVNGKCYYFDNNGYMMAN
TWVGDSYVDASGAWIPGKQKPTPGWKVVSGRWKYMESNGEYVKSRWLLVGSTWYHFDANG
WMQTGWLLTGNTWYYLESSGAMIAGRWQWINGKCYYFDNNGCMASDTWIGNWYVDANGAW
IPDKQKLTNIMGTSSVTALQLSNFYKAMNKKYPSYYSQNDKEAKTIEDFCKIYVEECKKE
GVRVEVAFAQAMVETGYLSFGGDVDISQYNFAGMGATGNGVKGNSFSTVRLGIRAQVQHL
KCYASTDKLNGDRVDPRWGEWLRGKAPYVEWLSIPNNPYGTGWAGSATYAEVIKGIMENI
NKYN

Enzyme Prediction     

No EC number prediction in MGYG000000206_00113.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH73	830	961	1.2e-17	0.9765625

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02696	MurNAc-LAA	6.56e-58	245	443	1	172	N-acetylmuramoyl-L-alanine amidase or MurNAc-LAA (also known as peptidoglycan aminohydrolase, NAMLA amidase, NAMLAA, Amidase 3, and peptidoglycan amidase; EC 3.5.1.28) is an autolysin that hydrolyzes the amide bond between N-acetylmuramoyl and L-amino acids in certain cell wall glycopeptides. These proteins are Zn-dependent peptidases with highly conserved residues involved in cation co-ordination. MurNAc-LAA in this family is one of several peptidoglycan hydrolases (PGHs) found in bacterial and bacteriophage or prophage genomes that are involved in the degradation of the peptidoglycan. In Escherichia coli, there are five MurNAc-LAAs present: AmiA, AmiB, AmiC and AmiD that are periplasmic, and AmpD that is cytoplasmic. Three of these (AmiA, AmiB and AmiC) belong to this family, the other two (AmiD and AmpD) do not. E. coli AmiA, AmiB and AmiC play an important role in cleaving the septum to release daughter cells after cell division. In general, bacterial MurNAc-LAAs are members of the bacterial autolytic system and carry a signal peptide in their N-termini that allows their transport across the cytoplasmic membrane. However, the bacteriophage MurNAc-LAAs are endolysins since these phage-encoded enzymes break down bacterial peptidoglycan at the terminal stage of the phage reproduction cycle. As opposed to autolysins, almost all endolysins have no signal peptides and their translocation through the cytoplasmic membrane is thought to proceed with the help of phage-encoded holin proteins. The amidase catalytic module is fused to another functional module (cell wall binding module or CWBM) either at the N- or C-terminus, which is responsible for high affinity binding of the protein to the cell wall.
pfam01520	Amidase_3	3.10e-52	246	442	1	172	N-acetylmuramoyl-L-alanine amidase. This enzyme domain cleaves the amide bond between N-acetylmuramoyl and L-amino acids in bacterial cell walls.
NF033930	pneumo_PspA	1.14e-51	474	781	445	660	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
NF033930	pneumo_PspA	2.68e-48	453	675	465	660	pneumococcal surface protein A. The pneumococcal surface protein proteins, found in Streptococcus pneumoniae, are repetitive, with patterns of localized high sequence identity across pairs of proteins given different specific names that recombination may be presumed. This protein, PspA, has an N-terminal region that lacks a cross-wall-targeting YSIRK type extended signal peptide, in contrast to the closely related choline-binding protein CbpA which has a similar C-terminus but a YSIRK-containing region at the N-terminus.
COG0860	AmiC	7.49e-47	245	448	44	230	N-acetylmuramoyl-L-alanine amidase [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRO35993.1	1.23e-105	53	450	77	478
QBF76227.1	1.23e-105	53	450	77	478
ASM68313.1	3.20e-96	58	958	30	1048
QYX25930.1	9.15e-90	74	963	97	1140
QNM00238.1	3.58e-84	79	953	43	611

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5J72_A	2.02e-21	246	442	454	633	ChainA, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630],5J72_B Chain B, Putative N-acetylmuramoyl-L-alanine amidase,autolysin cwp6 [Clostridioides difficile 630]
3NE8_A	6.36e-17	241	442	2	220	Thecrystal structure of a domain from N-acetylmuramoyl-l-alanine amidase of Bartonella henselae str. Houston-1 [Bartonella henselae]
5EMI_A	3.08e-16	241	445	2	177	ChainA, Cell wall hydrolase/autolysin [Nostoc punctiforme PCC 73102]
4BIN_A	3.51e-15	244	446	174	393	Crystalstructure of the E. coli N-acetylmuramoyl-L-alanine amidase AmiC [Escherichia coli K-12]
4RN7_A	1.47e-14	246	452	6	187	ChainA, N-acetylmuramoyl-L-alanine amidase [Clostridioides difficile 630]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q06320	1.45e-28	244	450	2	179	Sporulation-specific N-acetylmuramoyl-L-alanine amidase OS=Bacillus subtilis (strain 168) OX=224308 GN=cwlC PE=1 SV=1
P37134	8.65e-28	244	450	2	179	N-acetylmuramoyl-L-alanine amidase CwlM OS=Bacillus licheniformis OX=1402 GN=cwlM PE=3 SV=1
O48471	2.79e-26	245	450	4	185	Endolysin OS=Bacillus phage SPP1 OX=10724 GN=25 PE=4 SV=1
O51481	1.01e-22	831	964	73	199	Uncharacterized protein BB_0531 OS=Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31) OX=224326 GN=BB_0531 PE=3 SV=1
P54525	7.85e-21	246	446	32	204	Uncharacterized protein YqiI OS=Bacillus subtilis (strain 168) OX=224308 GN=yqiI PE=3 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000334	0.998807	0.000323	0.000168	0.000169	0.000149

TMHMM  Annotations     

start	end
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